A5061901447- Sleep and Wakefulness Research
- Sleep and related disorders
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Regulation of Appetite and Obesity
- Advanced Biosensing Techniques and Applications
- Acoustic Wave Resonator Technologies
- Adenosine and Purinergic Signaling
- Monoclonal and Polyclonal Antibodies Research
- Neural dynamics and brain function
- Ferroelectric and Piezoelectric Materials
- Laser-induced spectroscopy and plasma
- Neuropeptides and Animal Physiology
- Solid State Laser Technologies
- Neurogenesis and neuroplasticity mechanisms
- Bacterial Infections and Vaccines
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Systemic Lupus Erythematosus Research
- Mast cells and histamine
- Tryptophan and brain disorders
- Advanced Fiber Laser Technologies
- Obstructive Sleep Apnea Research
- Renal Diseases and Glomerulopathies
Tsukuba International University
2014-2024
University of Tsukuba
2014-2024
Beth Israel Deaconess Medical Center
2013-2014
Harvard University
2013
Osaka University
1996-2008
Keio University
2006
The University of Tokyo
2006
Nara Institute of Science and Technology
2006
Stanley Electric (Japan)
2005
Abstract Sleep control is ascribed to a two-process model, widely accepted concept that posits homoeostatic drive and circadian process as the major sleep-regulating factors. Cognitive emotional factors also influence sleep–wake behaviour; however, precise circuit mechanisms underlying their effects on sleep are unknown. Previous studies suggest adenosine has role affecting behavioural arousal in nucleus accumbens (NAc), brain area critical for reinforcement reward. Here, we show...
Adenosine has been proposed to promote sleep through A 1 receptors (A R's) and/or 2A in the brain. We previously reported that mediate sleep-promoting effect of prostaglandin D 2 , an endogenous sleep-inducing substance, and activation these induces blockade them by caffeine results wakefulness. On other hand, R suggested increase inhibition cholinergic region basal forebrain. However, role target sites sleep–wake regulation remained controversial. In this study, immunohistochemistry...
Narcolepsy is characterized by excessive sleepiness and cataplexy, sudden episodes of muscle weakness during waking that are thought to be an intrusion rapid eye movement sleep atonia into wakefulness. One the most striking aspects cataplexy it often triggered strong, generally positive emotions, but little known about neural pathways through which emotions trigger atonia. We hypothesized amygdala functionally important for because has a role in processing emotional stimuli contains neurons...
Narcolepsy is characterized by chronic sleepiness and cataplexy, episodes of profound muscle weakness that are often triggered strong, positive emotions. with cataplexy caused a loss orexin (also known as hypocretin) signaling, but almost nothing about the neural mechanisms through which emotions trigger cataplexy. Using knock-out mice model narcolepsy, we found palatable foods, especially chocolate, markedly increased activated neurons in medial prefrontal cortex (mPFC). Reversible...
Sleep–wake behavior is controlled by a wide range of neuronal populations in the mammalian brain. Although ventral midbrain/pons (VMP) area suggested to participate sleep–wake regulation, mechanisms have remained unclear. Here, we found that nonspecific cell ablation or selective GABAergic neurons expressing diphtheria toxin fragment A VMP male mice induced large increase wakefulness lasted at least 4 weeks. In contrast, dopaminergic had little effect on wakefulness. Chemogenetic inhibition...
Recording of the epidural electroencephalogram (EEG) and electromyogram (EMG) in small animals, like mice rats, has been pivotal to study homeodynamics circuitry sleep-wake regulation. In many laboratories, a cable-based sleep recording system is used monitor EEG EMG freely behaving combination with computer software for automatic scoring vigilance states on basis power spectrum analysis data. A description this detailed herein. Steel screws are implanted over frontal cortical area parietal...
Scope We found that rubiscolin‐6, a δ opioid agonist peptide derived from d ‐ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco), major protein of green leaves, stimulates food intake after oral administration in mice. therefore investigated its mechanism. Methods and results Orexigenic activity rubiscolin‐6 was blocked by central naltrindole, an antagonist for receptor, suggesting orally administered via receptor activation. The orexigenic inhibited celecoxib, cyclooxygenase ( COX )‐2...
Background: Insomnia is associated with psychiatric illnesses such as bipolar disorder or schizophrenia. Treating insomnia improves psychotic symptoms severity, quality of life, and functional outcomes. Patients disorders are often dissatisfied the available therapeutic options for their insomnia. In contrast, positive allosteric modulation adenosine A 2A receptors (A Rs) leads to slow-wave sleep without cardiovascular side effects in contrast R agonists. Methods: We investigated hypnotic...
Abstract Histamine H 1 receptor (H R) in the central nervous system plays an important role various functions, including learning and memory, aggression, feeding behaviors, wakefulness, as evidenced by studies utilizing R knockout mice pharmacological interventions. Although previous have reported widespread distribution of brains rats, guinea pigs, monkeys, humans, detailed mouse brain remains unclear. This study provides a comprehensive description mRNA using two recently developed...
Background The role of the diffusible messenger nitric oxide (NO) in regulation pain transmission is still a debate matter, pro-nociceptive and/or anti-nociceptive. S-Nitrosylation, reversible post-translational modification selective cysteine residues proteins, has emerged as an important mechanism by which NO acts signaling molecule. occurrence S-nitrosylation spinal cord and its targets that may modulate remain unclarified. “biotin-switch” method matrix-assisted laser...
Injection of nanomolar amounts prostaglandin D2 (PGD2) into the rat brain has dose and time-dependent somnogenic effects, PGD2-induced sleep is indistinguishable from physiologic sleep. Sleep-inducing PGD2 produced in by lipocalin-type synthase (LPGDS). Three potential intracranial sources LPGDS to produce have been identified: oligodendrocytes (OD), choroid plexus (CP), leptomeninges (LM). We aimed at identification site synthesis therefore, generated a transgenic mouse line with gene...
Abstract Lipocalin-type prostaglandin (PG) D synthase (L-PGDS) is responsible for the production of PGD 2 in adipocytes and selectively induced by a high-fat diet (HFD) adipose tissue. In this study, we investigated effects HFD on obesity insulin resistance two distinct types adipose-specific L-PGDS gene knockout (KO) mice: fatty acid binding protein 4 ( fabp4 , aP2 ) -Cre/L-PGDS flox/flox adiponectin AdipoQ mice. The was deleted premature stage former strain after maturation latter strain....
Individuals with the neuropsychiatric disorder mania exhibit hyperactivity, elevated mood, and a decreased need for sleep. The brain areas neuronal populations involved in mania-like behaviors, however, have not been elucidated. In this study, we found that ablating ventral medial midbrain/pons (VMP) GABAergic neurons induced behaviors mice, including anti-depressive reduced anxiety, increased risk-taking distractibility, an extremely shortened sleep time. Strikingly, these mice also showed...
Although recent advances in fluorescence-based technologies, such as protein microarrays, have made it possible to analyse more than 10,000 proteins at once, there is a bottleneck the step of preparation large numbers fluorescently labelled for comprehensive analysis protein-protein interactions. Here we describe two independent methods high-throughput fluorescence-labelling full-length cDNA products their C-termini using reconstituted translation system containing fluorescent puromycin. For...
Abstract Bismuth-layer-structured ferroelectric thin films, SrBi2Ta2O9 and Bi4Ti3O12, have been prepared by laser ablation method on both Pt sheets Si wafers at low temperatures of 400 ∼ 500°C. These films characterized XRD, XPS, AFM, C-V, D-E hysteresis J-V measurement. a good (105) preferential orientation, Bi4Ti3O12 (117) c-axis orientation these substrates. Ferroelectric film-SiO2-Si structures show C-V curve owing to surface potential controlled the hysteresis. is obtained in film...