A5007136116- Stress Responses and Cortisol
- Dermatology and Skin Diseases
- Food Allergy and Anaphylaxis Research
- Adrenal Hormones and Disorders
- Helicobacter pylori-related gastroenterology studies
- Neurobiology and Insect Physiology Research
- Synthesis and Biological Evaluation
- Advanced biosensing and bioanalysis techniques
- Animal testing and alternatives
- Asthma and respiratory diseases
- Urticaria and Related Conditions
- Antioxidant Activity and Oxidative Stress
- Human Health and Disease
- Fatty Acid Research and Health
- Animal Behavior and Welfare Studies
- Inflammatory Bowel Disease
- Monoclonal and Polyclonal Antibodies Research
The University of Tokyo
2018-2025
Upon immediate allergic inflammation, activated mast cells produce abundant bioactive lipid mediator prostaglandin (PG) D2. PGD2 is metabolised and excreted in urine as tetranor-PGDM. We have previously reported urinary tetranor-PGDM a sensitive biomarker for food allergy (FA) reactions children [1]. However, the production profile of other mediators FA patients remains unknown. Bioactive lipids are produced by enzyme-dependent or independent metabolism polyunsaturated fatty acids (PUFAs)...
Scratching is one of the most important behaviours in experimental animals because it can reflect itching and/or psychological stress. Here, we aimed to establish a novel method detect scratching using deep neural network. was elicited by injecting chemical pruritogen lysophosphatidic acid back mouse, and behaviour recorded standard handy camera. Images showing differences between two consecutive frames each video were generated, frame manually labelled as or not. Next, convolutional...
Although several studies have revealed the role of different lipid mediators in colitis, comprehensive analysis their production across phases colitis remained unclear. Here, we performed following dextran sodium sulfate (DSS)-induced model using LC-MS/MS. Oral administration 2% DSS mice for 4 days resulted severe intestinal inflammation by day 7, which gradually subsided 18. Based on disease scoring index (assigned basis fecal condition and weight loss), defined as induction (days 0-4),...
Grooming is a common behavior for animals to care their fur, maintain hygiene, and regulate body temperature. Since various factors, including stressors genetic mutations, affect grooming quantitatively qualitatively, the assessment of important understand status experimental animals. However, current detection methods are time-consuming, laborious, require specialized equipment. In addition, they generally cannot discriminate microstructures such as face washing licking. this study, we...
Since the scratching assessment is only way to estimate itching sensation in non-verbal experimental animal, it utilized various research fields. However, current methods depend on human observation, which laborious, low-throughput, and includes observer-bias. We here aimed establish an automated detection method of mice using neural network, artificial intelligence technology excels image recognition.
Tetranor-PGDM is a metabolite of PGD2. Urinary tetranor-PGDM levels were reported to be increased in some diseases, including food allergy, Duchenne muscular dystrophy, and aspirin-intolerant asthma. In this study, we developed monoclonal antibody (MAb) competitive enzyme immunoassay (EIA) for measuring tetranor-PGDM. Spleen cells isolated from mice immunized with utilized generate Ab-producing hybridomas. We chose hybridomas purified MAb against develop EIA. The assay evaluated the optimal...