A5086643487- Gut microbiota and health
- Probiotics and Fermented Foods
- Immune Cell Function and Interaction
- Mast cells and histamine
- Digestive system and related health
- Microbial Metabolites in Food Biotechnology
- Asthma and respiratory diseases
- Helicobacter pylori-related gastroenterology studies
- Pediatric health and respiratory diseases
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Food Allergy and Anaphylaxis Research
- Infant Nutrition and Health
- T-cell and B-cell Immunology
- Polyamine Metabolism and Applications
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- IL-33, ST2, and ILC Pathways
- Clinical Nutrition and Gastroenterology
- Escherichia coli research studies
- Polysaccharides and Plant Cell Walls
- Glycosylation and Glycoproteins Research
- Olfactory and Sensory Function Studies
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
Nihon University
2016-2025
The University of Tokyo
1997-2007
Probiotic supplements induce immunological responses in the host, and dietary fructooligosaccharides (FOS) stimulate growth of selected intestinal microflora. In this study we investigated influences orally administrated FOS. BALB/c mice were administered 0-7.5% FOS for 6 weeks, mucosal immune measured. 2.5%-FOS group, fecal IgA was significantly increased. secretion by Peyer's patch (PP) cells upregulated a dose-dependent way response to CD4+ T from PP showed increase production...
Intestinal epithelial cells (IECs) are continuously exposed to large numbers of commensal bacteria but relatively insensitive them, thereby averting an excessive inflammatory reaction. In this study, we show that the low responsiveness human IEC lines LPS was mainly brought about by a down-regulation TLR4 gene transcription. Additionally, presence IEC-specific repressor element in 5' region and binding NF shown. The transcription factor ZNF160, which expressed more abundantly LPS-low...
Intestinal epithelial cells (IECs) are continuously exposed to large numbers of commensal bacteria but relatively insensitive them, thereby averting an excessive inflammatory reaction. We have previously reported that the hyporesponsiveness a human IEC line LPS was primarily result down-regulation TLR4 gene transcription through epigenetic mechanisms. In present study we show DNA methylation in 5' region is significantly higher IECs than splenic vivo. The shown be dependent on...
It has been demonstrated that intestinal commensal bacteria induce immunoglobulin (Ig) A production by promoting the development of gut-associated lymphoid tissues in small intestine. However, precise mechanism whereby these modulate IgA large intestine, which harbors majority commensals, is poorly understood. In addition, it not known intestine and To address issues, we generated gnotobiotic mice mono-associated with different murine inoculating germ-free (GF) Lactobacillus johnsonii or...
The intestinal tract contains many commensal bacteria that modulate various physiological host functions. Dysbiosis of triggers dysfunction the epithelial barrier, leading to induction or aggravation inflammation. To elucidate whether microRNA plays a role in microbiome-dependent barrier regulation, we compared transcripts cells (IECs) from conventional and germ-free mice found induced expression miR-21-5p IECs. increased permeability up-regulated ADP ribosylation factor 4 (ARF4), small...
The soluble and insoluble fractions obtained after sonication centrifugation of Bifidobacterium adolescentis M101-4 cells were examined, both these exhibited mitogenic activity in an assay murine splenocytes Peyer's patch vitro. fraction was further treated by a 6-step procedure involving proteinase K-treatment, ultrafiltration with 50-kDa cut-off molecular-sieving membrane, anion-exchange chromatography, dialysis, through 6-kDa membrane filter, gel-filtration to yield high molecular weight...
Abstract This study explores how the odor of oregano and its active component, carvacrol, influence salt preference in mice. Using a two-bottle choice test (distilled water vs. 0.15 M NaCl), 66 C57BL/6 J mice were exposed to odor. Female showed significant reduction saline intake with or carvacrol exposure, while effect was lower males. Carvacrol identified dried using GC–MS HS–SPME. Neurologically, increased c-Fos immunoreactivity ventral bed nucleus stria terminalis (BNST), region...
The gut mucosal immune system is crucial in host defense against infection by pathogenic microbacteria and viruses via the production of IgA. Previous studies have shown that intestinal commensal bacteria enhance IgA production. However, it poorly understood how these induce which genera effectively. In this study, we compared immunomodulatory effects Bacteroides Lactobacillus on Peyer's patches lymphocytes. lymphocytes co-cultured with was higher than Lactobacillus. addition, expression...
Abstract Inactivated influenza vaccines have two formulations, whole- and split-virion types; however, how differential formulations impact their booster effects remain unknown. In this study, we demonstrate that whole-virion recall waves of Ab responses, early T cell–independent (TI) late cell–dependent whereas elicit the response only. Notably, higher-affinity Abs with improved neutralizing activity are provided from TI response, which emphasizes important contribution...
<i>Background:</i> Although much attention has been focused on the anti-allergic effects of probiotics, their mode action is not fully understood. Mast cells, which play a central role in inducing allergic inflammation, are potential targets probiotics given recent discovery that they express Toll-like receptors (TLRs), pattern recognition for microbial components. In this study, we examined whether reactions mast cells modulated by stimulation through TLR2....
Lactobacillus GG and L. gasseri TMC0356 were examined for their potential to alter the immune responses of murine PP cells in vitro vivo. characteristically stimulated production IL-12, IL-6, IFN-gamma IgA from isolated vitro. Anatomical analysis indicated uptake these bacteria by tissue after giving orally mice. Isolated exposed vivo secreted more IFN-gamma, IL-6 total IgA, whereas those did not exhibit altered terms cytokine production. Therefore, two might different immunodulatory effects...
Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated cellular mechanisms through which FOS modulate antigen-specific CD4+ T cell responses in food allergy, using OVA23-3 mice.OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA for 1 week. Body weight mucosal mast protease serum measured as indicator inflammation. Single-cell suspensions...
Cyclooxygenase-2 is expressed early in colon carcinogenesis and plays crucial role the progress of disease. Recently, we found that α-lactalbumin had anti-inflammatory activity by inhibiting cyclooxygenase-2. In experiment 1, investigated effects on initiated with azoxymethane (AOM) followed promotion dextran sodium sulfate (DSS) mice. Dietary treatment decreased fecal occult blood score at 3 days after DSS intake. α-Lactalbumin also tumor week 9. 2, AOM-treated mice were sacrificed 7 The...
The intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. Epidemiologic studies have identified associations between alterations in composition intestinal microbiota and development allergic disease. However, cellular molecular mechanisms underlying these effects remain be determined. Here, we show heat-killed suppressed degranulation mast cells vitro MyD88-independent manner. In particular, Enterococcus faecalis showed strongest...
CCAAT/enhancer binding protein alpha (C/EBPα) is a transcription factor that influences immune cell fate and differentiation. However, the effect of C/EBPα on mast cells not fully understood. In this study, we showed suppressed granule formation in increased macrophage inflammatory (MIP)‐2 production from upon bacterial stimulation. These results indicate regulates balance between allergic response innate cells. Furthermore, stimulation with Lactobacillus casei JCM1134 T strain during late...
Background and objective The mechanism inducing either inflammation or tolerance to orally administered food allergens remains unclear. To investigate this we analyzed mouse models of allergy (OVA23-3) (DO11.10 [D10]), both which express ovalbumin (OVA)-specific T-cell receptors. Methods OVA23-3, recombination activating gene (RAG)-2-deficient OVA23-3 (R23-3), D10, RAG-2-deficient D10 (RD10) mice consumed a diet containing egg white (EW diet) for 2–28 days. Interleukin (IL)-4 production by...