Allyl sulfides are characteristic flavor components obtained from garlic. These thought to be responsible for their epidemiologically proven anticancer effect on garlic eaters. This study was aimed at clarifying the molecular basis of this by using human colon cancer cell lines HCT-15 and DLD-1. The growth cells significantly suppressed diallyl trisulfide (DATS, IC50 = 11.5 microM, DLD-1 13.3 microM); however, neither monosulfide nor disulfide showed such an effect. proportion that residing...

This study verifies the instability of garlic (Allium sativum L.)-derived allyl 2-propenylthiosulfinate (allicin) in various aqueous and ethanolic solutions as well vegetable oil through chemical biological analyses performed simultaneously. Crushed fresh cloves generated antibacterial activity chemically detectable allicin, a major principle, both declined on daily basis at room temperature, showing half-lives about 6 11 days, respectively. Allicin was more stable 20% alcohol than water,...

Allicin (allyl 2-propenethiosulfinate), an antibacterial principle of garlic, has drawn much attention, since it potent antimicrobial activity against a range microorganisms, including methicillin-resistant Staphylococcus aureus. There have been many reports on the properties allicin, but no quantitative comparison activities between freshly prepared garlic extract and clinically useful antibiotics performed. To verify substantial effect aqueous extract, we compared with those allicin...

We previously demonstrated that cinnamon extract (CE) ameliorates type 1 diabetes induced by streptozotocin in rats through the up-regulation of glucose transporter 4 (GLUT4) translocation both muscle and adipose tissues. This present study was aimed at clarifying detailed mechanism(s) with which CE increases uptake vivo cell culture systems using 3T3-L1 adipocytes C2C12 myotubes vitro. Specific inhibitors key enzymes insulin signaling AMP-activated protein kinase (AMPK) pathways, as well...

Effects of pre-germinated brown rice (PGBR) on streptozotocin-induced diabetic rats were studied. The feeding a PGBR diet to ameliorated the elevation blood glucose and PAI-1 concentrations significantly, tended decrease plasma lipid peroxide in comparison with fed white diet. These results suggest that intake instead is effective for prevention vascular complications.

It has long been believed that an intake of cinnamon (Cinnamomum zeylanicum) alleviates diabetic pathological conditions. However, it is still controversial whether the beneficial effect insulin-dependent or insulin-mimetic. This study was aimed at determining insulin-independent cinnamon. Streptozotocin-induced rats were divided into four groups and orally administered with aqueous extract (CE) for 22 d. The had taken CE a dose more than 30 mg/kg/d rescued from their hyperglycemia...

The garlic-derived antibacterial principle, alk(en)yl sulfinate compounds, has long been considered as very short-lived substance. However, there are some data showing a rather more stable nature of allicin. We determined here the thermostability allicin by systematic analyses employing chemical quantification and an activity assay. Allicin in aqueous extract garlic was degraded stoichiometrically proportion to temperature; we estimated half-life be about year at 4 °C (from 1.8 mg/ml 0.9...

Journal Article Analysis of Volatile Components in Essential Oil Upland Wasabi and Their Inhibitory Effects on Platelet Aggregation Get access Hitomi Kumagai, Kumagai Department Agricultural Chemistry, College Agriculture Veterinary Medicine, Nihon University, 34–1, Shimouma 3-chome, Setagaya-ku, Tokyo 154, Japan Search for other works by this author on: Oxford Academic Google Scholar Naoki Kashima, Kashima Taiichiro Seki, Seki Hidetoshi Sakurai, Sakurai Kenji Ishii, Ishii Toyohiko Ariga...

The intake of pre-germinated brown rice (PR) instead white (WR) ameliorates the hyperglycemia. To clarify mechanism(s) to decrease post-prandial blood glucose concentration, effect water-soluble/oil-soluble fraction-depleted PR bran (termed as "DB"; which is destarched and defatted bran) on was compared with that full-fat (PB) or WR. test diets, WR diet, PB diet DB are containing identical amount available carbohydrate (1.5 g) were fed Wistar strain rats. Post-prandial concentration...

Alk(en)yl trisulfides (R-SSS-R′) are organosulfur compounds produced by crushed garlic and other Allium vegetables. We found that these exhibit potent anticancer effects through the reaction with microtubules, causing cell cycle arrest. Nine alk(en)yl including dimethyl trisulfide, diethyl dipropyl trisulfide (DPTS), dibutyl dipentyl diallyl (DATS), dibutenyl dipentenyl allyl methyl were synthesized added to cultures of HT-29 human colon cancer cells at a concentration 10 μM. The alkenyl...

Cyclooxygenase-2 is expressed early in colon carcinogenesis and plays crucial role the progress of disease. Recently, we found that α-lactalbumin had anti-inflammatory activity by inhibiting cyclooxygenase-2. In experiment 1, investigated effects on initiated with azoxymethane (AOM) followed promotion dextran sodium sulfate (DSS) mice. Dietary treatment decreased fecal occult blood score at 3 days after DSS intake. α-Lactalbumin also tumor week 9. 2, AOM-treated mice were sacrificed 7 The...

Garlic (Allium sativum L.) has long been used as a medicinal food. Indeed, garlic and its constituents have shown to possess potent regulatory activities in bodily functions, including blood coagulation, lipid metabolism, immunity xenobiotic metabolism. In this study, we aimed examine the anti-obesity effects of oil elucidate possible underlying mechanisms. For purpose, (GO; 80 mg/kg body weight, p.o.) or corn alone vehicle-control were administered male Sprague-Dawley rats every other day...

Scope Diallyl trisulfide (DATS), an organosulfur compound generates in crushed garlic, has various beneficial health effects. A growing body of evidence indicates that miRNAs are involved the pathology lifestyle diseases including obesity. The anti‐obesogenic effect garlic is previously reported; however, effects DATS on obesity, and relationship between compounds involvement miRNA remains unclear. Here, activity potential role a diet‐induced obesity rat model investigated. Methods Results...

Annexin (Anx) A3 increases and plays important roles in the signalling cascade hepatocyte growth cultured hepatocytes. However, no information is available on its expression role rat liver regeneration. In present study, AnxA3 was investigated to determine whether it also a protein mRNA level both increase after administration of carbon tetrachloride (CCl4) or 70% partial hepatectomy. isolated parenchymal hepatocytes, but not non-parenchymal cells, these regeneration models. hepatocytes CCl4...

Pre-germinated brown rice (PR) has been developed industrially in order to enhance the nutritional functions of its source material, (BR). The present study was aimed at clarifying effect PR on type 2 diabetes mellitus. We employed Otsuka Long-Evans Tokushima Fatty (OLETF) rats as a model type-2 OLETF were fed either or white (WR) from age 4 35 wk. Age-matched male (LETO) non-diabetic control also WR. HbA(1c) level significantly higher than that LETO rats. However, lower PR-fed WR-fed plasma...

We previously demonstrated that cinnamon extract (CE) alleviates streptozotocin-induced type 1 diabetes in rats. The present study aimed to elucidate the detailed molecular target of cultured adipocytes and epididymal adipose tissue 2 model mice. Two-dimensional gel electrophoresis was employed determine adipocytes. function Acyl-CoA synthetase long-chain family-1 (ACSL1), a identified this study, further investigated 3T3-L1 using specific inhibitors. Type mice (KK-Ay/TaJcl) were used...

Abstract Although the constitute activation of Src family kinases (Src) has been established as a poor prognostic factor in several types cancer, role renal cell carcinoma (RCC) not defined. This study aimed to determine whether could contribute appearance malignant phenotypes RCC. The RCC was examined two human cancer lines, Caki‐1 from metastatic and ACHN primary activity cells higher than that cells, this difference corresponded PP1 (a inhibitor)‐induced cytotoxicity on cells. between did...