A5000185270- Pharmacology and Obesity Treatment
- Pharmacological Effects of Natural Compounds
- Glycosylation and Glycoproteins Research
- Liver physiology and pathology
- Protein purification and stability
- S100 Proteins and Annexins
- Natural product bioactivities and synthesis
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Chromatography in Natural Products
- Cancer Treatment and Pharmacology
- Mass Spectrometry Techniques and Applications
- Chemical synthesis and alkaloids
- Pain Mechanisms and Treatments
- Immune Response and Inflammation
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- PI3K/AKT/mTOR signaling in cancer
- Advanced Proteomics Techniques and Applications
- Complementary and Alternative Medicine Studies
- TGF-β signaling in diseases
- Neonatal Respiratory Health Research
- Plant biochemistry and biosynthesis
- Berberine and alkaloids research
- Phytochemicals and Antioxidant Activities
National Institute of Health Sciences
2012-2025
National Institutes of Health
2000-2011
Nihon University
2007
National Institute of Health Sciences
2001
Tokyo Institute of Technology
1999
Meiji (Japan)
1991
An anticoagulant protein, factor IX/factor X-binding protein (IX/X-bp), isolated from the venom of Trimeresurus flavoviridis, binds with IX and X in presence Ca2+ a 1 to stoichiometry (Atoda, H., Morita, T. (1989) J. Biochem. (Tokyo) 106, 808-813). Analysis S-pyridylethylated IX/X-bp by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed 16.0-kDa band (designated A chain) 15.5-kDa B chain). These two chains were separated reversed-phase high performance liquid chromatography,...
It is generally accepted that the primary pharmacological activities and adverse effects of Ephedra Herb are caused by ephedrine alkaloids. Interestingly, our research shows also has alkaloid-independent actions, such as c-MET inhibitory activity. This study describes preparation an alkaloids-free extract (EFE) ion-exchange column chromatography, well in vitro vivo evaluation its actions toxicity. We confirmed EFE suppressed hepatocyte growth factor (HGF)-induced cancer cell motility...
Several Ephedra Herb-containing Kampo medicines are common initial treatments for various infections; however, the ephedrine alkaloids in Herb can cause side effects by stimulating adrenergic receptors. Accordingly, an alkaloids-free Extract (EFE) has been developed. This study aimed to evaluate whether EFE be used effectively and safely patients with mild coronavirus disease 2019 (COVID-19). We randomized COVID-19 receive equivalent 6 g of per day or a placebo 14 days. The primary efficacy...
Nine known compounds: trans-cinnamic acid, catechin, syringin, epicatechin, symplocoside, kaempferol 3-O-rhamnoside 7-O-glucoside, isovitexin 2-O-rhamnoside, herbacetin and pollenitin B a new flavonoid glycoside, characterized as 7-O-neohesperidoside (1) on the basis of spectroscopic analysis chemical evidence, were isolated from traditional crude drug, "Ephedra herb extract". Compound 1 had no effects HGF-induced motility, whereas herbacetin, which is an aglycone 1, significantly inhibited it.
Ephedrine alkaloids (EAs) have been considered the main pharmacologically active substances in Ephedra Herb (, Mao; EH) since they were first identified by Prof. N. Nagai, and are known to induce palpitation, hypertension, insomnia, dysuria as side effects. Therefore, administration of drugs containing EH patients with cardiovascular-related diseases is severely contraindicated. While our previous studies suggest that some effects may not be due EAs, considering their would expedient develop...
We previously demonstrated that high-performance liquid chromatography with electrospray ionization mass spectrometry (LC/MS) equipped a graphitized carbon column (GCC) is useful for the structural analysis of carbohydrates in glycoproteins. Using LC/MS GCC, sulfated N-linked oligosaccharides were found erythropoietin (EPO) expressed baby hamster kidney cells. Sulfation occurs part EPO. Sulfated monosaccharide residue oligosaccharide was determined by exoglycosidase digestion followed sugar...
Ephedrae herba suppresses hepatocyte growth factor-induced cancer cell motility by inhibiting tyrosine phosphorylation of the factor receptor, c-Met, and PI3K/Akt pathway. Moreover, directly inhibits tyrosine-kinase activity c-Met. Ephedrine-type alkaloids, which are active component herba, do not affect factor-c-Met-Akt signalling, prompting us to study other molecules in herb. We recently discovered herbacetin glycosides found that their aglycon, herbacetin, signalling. This revealed a...
We report for the first time that ephedrine alkaloids-free Ephedra Herb extract (EFE) directly inhibits replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro and addition EFE to culture medium before viral infection reduces virus titers supernatant SARS-CoV-2, including those variant strains, by more than 99%, 24 h after infection. The macromolecule condensed-tannin, which is main active ingredient responsible anticancer, pain suppression, anti-influenza...
Ephedrine alkaloids-free Ephedra Herb extract (EFE) has been developed to eliminate the adverse effects caused by ephedrine alkaloid-induced sympathetic hyperactivation. Previously, we reported that EFE possesses analgesic, anti-influenza, and cancer metastatic inhibitory at comparable levels of (EHE). However, it not yet demonstrated is free from known side EHE, such as excitation, insomnia, arrhythmias. In this study, incidence these was compared between mice administered EHE those EFE....
Annexin (Anx) A3 increases and plays important roles in the signalling cascade hepatocyte growth cultured hepatocytes. However, no information is available on its expression role rat liver regeneration. In present study, AnxA3 was investigated to determine whether it also a protein mRNA level both increase after administration of carbon tetrachloride (CCl4) or 70% partial hepatectomy. isolated parenchymal hepatocytes, but not non-parenchymal cells, these regeneration models. hepatocytes CCl4...
The analgesic effect of Ephedra Herb (EH) is believed to be derived from the anti-inflammatory action pseudoephedrine (Pse). We recently reported that ephedrine alkaloids-free EH extract (EFE) attenuates formalin-induced pain same level as achieved by (EHE), which suggests may not due alkaloids (EAs). To examine contribution EAs EH, mice were injected with formalin induce a biphasic reaction (first phase, 0-5 min; second 10-45 min) at various time points after oral administration following...
Previously, we reported that the c-Met inhibitory effect of Ephedra Herb extract (EHE) is derived from ingredients besides ephedrine alkaloids. Moreover, analgesic and anti-influenza activities EHE alkaloids-free (EFE) have been recently. In this study, examined fractions containing kinase activity with EFE, elucidated structural characteristics active fractions. Significant was observed in 30, 40, 50% methanol (MeOH) eluate obtained water using Diaion HP-20 column chromatography. Similarly,...
Kampo medicines containing Ephedra Herb (EH) such as eppikajutsubuto and makyoyokukanto are used to treat myalgia, arthralgia, rheumatism. The analgesic effects of these attributed the anti-inflammatory action EH. However, molecular mechanism effect EH remains be clarified. In this study, extract (EHE) on transient receptor potential vanilloid 1 (TRPV1), a nonselective ligand-gated cation channel, which plays an essential role in nociception sensory neurons, were investigated using...
Ganglioside GD1a, which is highly expressed in poorly metastatic FBJ-S1 cells, has been shown to inhibit the serum-induced migration capability of FBJ-LL cells. In present study, capacity cells adhere vitronectin was found be about half that Pre-treatment with GD1a decreased this by 30% control, whereas GM1-pre-treatment caused only a 10% decrease, indicating specifically inhibits cell adhesion vitronectin. Since contain almost no transfectants capable expressing varying degrees were...
Ephedra Herb is an important crude drug; it used in various Traditional Japanese Medicine (Kampo) formulations. Its significant pharmacological effects have been believed to be attributed ephedrine and pseudoephedrine, which sometimes induce adverse effects. On the other hand, has reported that some of these are not dependent on or pseudoephedrine. Ephedrine alkaloids‐free extract newly developed. It analgesic, anti‐influenza, antimetastatic This clinical trial was aimed at verifying...
Annexin A3 is a member of the lipocortin/annexin family, which binds to phospholipids and membranes in Ca2+-dependent manner. Although annexin has various functions vitro, its cellular significance completely unknown. not found rat liver vivo. In present study, we investigated expression primary cultured parenchymal hepatocytes. protein was detected 48-h, but 2.5-h, hepatocytes using Western blot analysis. The level further increased after an additional 24 h culture. mRNA 2.5-h 22 start...
We previously reported that ganglioside GD1a, which is highly expressed in poorly metastatic FBJ-S1 cells, inhibits the serum-induced motility of FBJ-LL cells and potential completely suppressed by enforced GD1a expression (Hyuga et al., Int J Cancer 1999;83:685–91). recently discovered hepatocyte growth factor (HGF) induces cell motility. In present study, HGF-induced was found to be one-thirtieth cells. This GD1a-expressing transfectants, were produced transfection with GM2/GD2 synthase...
Paclitaxel-resistant HuH-7 (HuH-7/PTX) cells were established by one-week exposure of to paclitaxel analyze the effects Kampo medicines on MDR-1-mediated multidrug resistance. HuH-7/PTX expressed high levels MDR-1 and efficiently exported calcein-acetoxymethylester (calcein-AM), which is a substrate MDR-1, suggesting that resist overexpressing MDR-1. We assessed 26 kinds medicine calcein-AM efflux assay using cells, results revealed takushato goreisan are potential inhibitors drug...