A5020705305- Carbohydrate Chemistry and Synthesis
- Vibrio bacteria research studies
- Immune Response and Inflammation
- Effects and risks of endocrine disrupting chemicals
- Orthopaedic implants and arthroplasty
- Polymer Science and PVC
- Glycosylation and Glycoproteins Research
- Aquaculture disease management and microbiota
- Animal testing and alternatives
- Biomedical Ethics and Regulation
- Total Knee Arthroplasty Outcomes
- 3D Printing in Biomedical Research
- Contact Dermatitis and Allergies
- Escherichia coli research studies
- Lipid Membrane Structure and Behavior
- Pharmaceutical Quality and Counterfeiting
- Safe Handling of Antineoplastic Drugs
- Probiotics and Fermented Foods
- Metabolomics and Mass Spectrometry Studies
- Food Allergy and Anaphylaxis Research
- Orthopedic Infections and Treatments
- Analytical Chemistry and Chromatography
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Engineering Technology and Methodologies
National Institute of Health Sciences
2010-2024
Smith College
2018
Syracuse University
2018
University of Memphis
2018
Texas A&M University
2018
Louisiana State University Health Sciences Center New Orleans
2018
John Wiley & Sons (United States)
2018
Pharmaceuticals and Medical Devices Agency
2016
National Institute of Infectious Diseases
1998-2002
Kanagawa Dental University
1995
The chemical structure of lipid A isolated from Porphyromonas gingivalis lipopolysaccharide was elucidated by compositional analysis, mass spectrometry, and nuclear magnetic resonance spectroscopy. hydrophilic backbone free found to consisted beta(1,6)-linked D-glucosamine disaccharide 1-phosphate. (R)-3-Hydroxy-15-methylhexadecanoic acid (R)-3-hydroxyhexadecanoic are attached at positions 2 3 the reducing terminal residue, respectively, 2' 3' nonreducing unit acylated with...
Assessment of skin irritation is an essential component the safety evaluation medical devices. OECD Test Guideline 439 describes use reconstructed human epidermis (RhE) as in vitro test system for classification by neat chemicals. An international round robin study was conducted to evaluate RhE method determination irritant potential device extracts. Four polymers and three non-irritant controls were obtained or developed that had demonstrated their suitability act positive negative samples....
We previously demonstrated that high-performance liquid chromatography with electrospray ionization mass spectrometry (LC/MS) equipped a graphitized carbon column (GCC) is useful for the structural analysis of carbohydrates in glycoproteins. Using LC/MS GCC, sulfated N-linked oligosaccharides were found erythropoietin (EPO) expressed baby hamster kidney cells. Sulfation occurs part EPO. Sulfated monosaccharide residue oligosaccharide was determined by exoglycosidase digestion followed sugar...
This study deals with bisphenol-A (BPA) analysis of the BPA-derived polymer pellets, polycarbonate (PC) and polysulfone (PS), in hemodialyzer casings made PC, leaching BPA from commercially available hemodialyzers into water bovine serum, using HPLC, GC-MS, LC-MS analyses, NMR spectroscopy. Total contents pellets each resin were 4.0 7.2 μg/g 34.5 (PS). Amounts released PC lacking PS hollow-fiber 11.7 13.7 ng/casing by extraction, 296 345 methanol extraction. On other hand, 3.78 to 141.8...
Abstract Contamination by endotoxin of nine kinds wound dressings made natural biomaterials (calcium alginate, collagen, chitin, and poly‐ L ‐leucine) was examined with the use water extracts. By applying Limulus amoebocyte lysate (LAL) test, high concentrations were detected in extracts from three products calcium alginate. These evoked fever rabbits induced release a proinflammatory (pyrogenic) cytokine, interleukin‐6 (IL‐6), human monocytic cells (MM6‐CA8). The effects disappeared when...
The chemical structure of the core oligosaccharide lipopolysaccharide isolated from Escherichia coli rough mutant F653, representing enterobacterial R3 type, was investigated by quantitative and methylation analyses, nuclear magnetic resonance spectroscopy, gas‐liquid chromatography/mass spectrometry, determined as image All sugars are present α‐pyranosides but anomeric configurations 3‐deoxy‐D‐ manno ‐octulopyranosonic acid (Kdo) residues could not be determined. third Kdo heptose‐linked...
A new sildenafil analogue, cyclopentynafil (1) and a tadalafil N-octylnortadalafil (2) were isolated from dietary supplement illegally marketed for erectile dysfunction. The structures of the analogues elucidated by using HPLC-photodiode array (PDA), LC-MS, high-resolution MS, NMR circular dichroism (CD). These compounds determined to be 5-[2-ethoxy-5-(4-cyclopentylpiperazin-1-ylsulfonyl)phenyl]-1-methyl-3-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one...
In this study, a titanium surface was chemically modified with calcium ions and assessed for its influence on osteogenic differentiation molecular responses of human mesenchymal stem cells (hMSCs). Titanium disks were treated NaOH (NaOH treatment), + CaCl2 (CaCl2 or Ca(OH)2 (Ca(OH)2 treatment). treatment caused significantly greater incorporation onto the apatite formation than treatment. The morphology hMSCs differed CaCl2- Ca(OH)2-treated disks. osteopontin (OPN) expression in cultured...
<i>Background:</i> Extensive analysis of allergenic proteins is generally time-consuming and labor-intensive. Accordingly, a rapid easy procedure for allergen identification required. As sequence information on genes accumulated in databases, it becoming easier to identify candidate protein using proteomic strategies, i.e. two-dimensional gel electrophoresis, site-specific fragmentation, mass spectrometry then database search. In this study, we evaluated the usefulness strategy...
A tadalafil analogue was detected in a dietary supplement marketed for tonic effect, along with hydroxyhomosildenafil and aminotadalafil. The isolated by preparative thin layer chromatography (TLC) its structure elucidated using high-performance liquid (HPLC), electrospray ionization-mass spectrometry (LC-ESI-MS), Fourier transform-ion cyclotron resonance-mass (FT-ICR-MS) nuclear magnetic resonance (NMR) spectroscopy. compound determined to be...
Introduction and methods: A pentaacyl diphosphoryl lipid molecule found in the isolated from Porphyromonas gingivalis lipopolysaccharide (LPS) was chemically synthesized, its characteristics were evaluated to reconfirm interesting bioactivities including low endotoxicity activity against LPS‐unresponsive C3H/HeJ mouse cells. Results: The synthesized P. (synthetic Pg‐LA) exhibited strong activities almost equivalent those of Escherichia coli ‐type synthetic (compound 506) all assays on...
Multipotent mesenchymal stem cells (MSCs) are widespread in adult organisms and implicated tissue maintenance repair, regulation of hematopoiesis, immunologic responses. Human (h)MSCs have applications engineering, cell-based therapy, medical devices but it is unclear how they respond to unfavorable conditions such as hypoxia or inflammation after vivo transplantation. Although endotoxin testing a requirement for evaluating the quality safety transplanted MSCs, there been no reports on dose...
<i>Background:</i> One of the latex allergens, Hev b 2, has β-1,3-glucanase activity. The entire sequence this allergen is already known. There one potential N-glycosylation site in molecule (<sup>27</sup>Asn). Heterogeneous glycosylation Asn residue could be a source multiplicity natural 2. Possible participation carbohydrate epitopes isoenzymes their IgE-binding capacity and cross-reactivity was investigated study. <i>Methods: </i>β-1,3-Glucanase were...
A new type of phosphodiesterase-5 (PDE-5) inhibitor, thioquinapiperifil (1), was found in dietary supplements. LC-MS analysis indicated that the supplements contain two major compounds. One identified as thiodenafil (synonym: thiosildenafil) by direct comparison with authentic compound. The other showed a molecular weight 448, and accurate mass measurement its elemental composition to be C24H28N6O1S1. Together, NMR spectrometric data revealed compound is an imidazoquinazoline derivative:...
This study examines the chemical, physicochemical, and biological properties of PVC sheets treated with UV irradiation on their surfaces to suppress elution a plasticizer, di-(2-ethylhexyl) phthalate (DEHP), for developing novel polyvinyl chloride (PVC) medical devices. The irradiated under conditions 1 (52.5 μW/cm(2), 136 J/cm(2)) 2 (0.45 mW/cm(2), 972 exhibited considerable toxicity in cytotoxicity tests chromosome aberration due generation DEHP oxidants, but no was detected sheet...
The relationship between chemical structure and biological activity of the lipid A from Porphyromonas gingivalis , which we recently isolated whose complete was determined [Kumada et al. (1995). J Bacteriol 177, 2098--2106], studied. exhibited endotoxic in all assay systems tested: Limulus gelation activity, lethal toxicity galactosamine-sensitized mice, mitogenicity mouse spleen cells induction nitric oxide (NO) tumour necrosis factor alpha (TNF) release both peritoneal macrophages J774-1...
Periodate-thiobarbituric acid reaction-positive substances were found in the strong hydrolysates of lipopolysaccharide (LPS) from Bacteroides gingivalis 381. They not identical to 2-keto-3-deoxyoctonate (KDO) high-voltage paper electrophoresis (HVPE), their electrophoretic mobilities relative KDO being 1.54 and 1.80, respectively. Alkaline phosphatase treatment HVPE demonstrated that they are some kind O-phosphorylated derivatives KDO; particular, slow-moving component is identical, at least...
Abstract This study deals with the development and performance evaluation of a positive reference material for hemolysis testing, which is used evaluating biological safety medical devices. Genapol X‐080, nonionic detergent, was selected as candidate hemolytic substance in survey 23 chemical compounds; it showed significant activity against rabbit defibrinated blood at concentrations more than 20 µg/mL. A polyvinyl chloride (PVC) sheet spiked 0.6% (w/w) compound exhibited weak direct contact...