A5102915416- Phytochemicals and Antioxidant Activities
- Free Radicals and Antioxidants
- Protein Hydrolysis and Bioactive Peptides
- Phytochemistry and Biological Activities
- Antioxidant Activity and Oxidative Stress
- Biochemical effects in animals
- Carcinogens and Genotoxicity Assessment
- Natural product bioactivities and synthesis
- Sesame and Sesamin Research
- Peptidase Inhibition and Analysis
- Toxic Organic Pollutants Impact
- Dye analysis and toxicity
- Fungal Plant Pathogen Control
- Food Allergy and Anaphylaxis Research
- Flavonoids in Medical Research
- Metabolomics and Mass Spectrometry Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Dyeing and Modifying Textile Fibers
- Garlic and Onion Studies
- Animal Genetics and Reproduction
- Effects and risks of endocrine disrupting chemicals
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
- Atherosclerosis and Cardiovascular Diseases
- Biochemical and biochemical processes
Nihon University
2012-2024
Fujikura (United States)
2009
University of California, Davis
2004-2005
Kyushu University
1991-1996
Takara (Japan)
1992
Japan Food Research Laboratories
1991
Journal Article Angiotensin I-converting Enzyme Inhibitory Peptides in an Alkaline Protease Hydrolyzate Derived from Sardine Muscle Get access Hiroshi Matsufuji, Matsufuji Department of Food Science and Technology, Faculty Agriculture, Kyushu University, 6–10–1 Hakozaki, Higashi-ku, Fukuoka 812, Japan Search for other works by this author on: Oxford Academic Google Scholar Toshiro Matsui, Matsui Eiji Seki, Seki JapanResearch Laboratories, Senmi Ekisu Co., Hirano, Ozu 795, Katsuhiro Osajima,...
Hydrolyzates which inhibit the angiotensin I-converting enzyme (ACE) were prepared from sardine muscle by Bacillus licheniformis alkaline protease. Considering practical application of preparations as a functional food material, best proteolytic conditions with respect to taste, solubility and ACE inhibitory activity 0.3 wt% addition 17-h proteolysis at 50°C pH 9.0. The under these had potent (IC50=0.26 mg protein/ml). Fractionation on an ODS column ethanol resulted in production more...
Allicin (allyl 2-propenethiosulfinate), an antibacterial principle of garlic, has drawn much attention, since it potent antimicrobial activity against a range microorganisms, including methicillin-resistant Staphylococcus aureus. There have been many reports on the properties allicin, but no quantitative comparison activities between freshly prepared garlic extract and clinically useful antibiotics performed. To verify substantial effect aqueous extract, we compared with those allicin...
The antioxidant ability of capsanthin and the fatty acid esters was examined by measuring free radical-oxidation methyl linoleate. To assess radical scavenging effect, production linoleate hydroperoxides decomposition capsanthins in reaction solution were measured HPLC. Capsanthin suppressed hydroperoxide formation as well β-carotene, lutein, zeaxanthin. Interestingly, decomposed more slowly than other carotenoids, effect found to last longer. Also, esterified partially and/or totally with...
The stability of red radish extract to light, heat, and hydrogen peroxide at different pH values (3, 5, 7) was examined, in which major anthocyanins were pelargonidin glycosides acylated with a combination p-coumaric, ferulic, or caffeic acids. light irradiation (fluorescence 5000 lx; 25 °C) indicated that the more stable lower than higher pH. HPLC analyses revealed diacylated 3 monoacylated anthocyanins. No significant difference degradation rates 5 observed, whereas p-coumaric ferulic...
Summary Five different coloured sweet peppers ( Capsicum annuum cv. Signal ), white, green, yellow, orange and red were analysed for total carotenoids, α ‐tocopherol, sugars (glucose, fructose sucrose), organic acids (citric ascorbic acids) anti‐oxidant properties. The mature fruits, ‘Signal Red’, Orange’ Yellow’ contained higher than the immature Green’ White’. Among Red’ was highest in carotenoids [9.15 mg (100 g) −1 of fresh weight] while ‐tocopherol [5.40 weight]. most acids. suppression...
Three iridoids (lamalbid (I1), sesamoside (I2) and shanzhiside methyl ester (I3)) seven polyphenols (cistanoside F (P1), chlorogenic acid (P2), pedalitin-6-O-laminaribioside (P3), pedaliin (P4), isoacteoside (P6), pedalitin (P7) martynoside (P8)) were identified in young sesame leaves (Sesamum indicum L.) other than the acteoside (P5) reported previously. P3 was a new compound, I1, I3, P2 P8 found species of Sesamum for first time. HPLC analyses revealed that compounds I1 (0.29–1.75% dry...
Abstract Sesame (Sesamum indicum L.) plants contain large amounts of acteoside, a typical phenylethanoid glycoside (PhG) that exhibits various pharmacological activities. Although there is increasing interest in the biosynthesis PhGs for improved production, pathway remains to be clarified. In this study, we established sesame-cultured cells and performed transcriptome analysis methyl jasmonate (MeJA)–treated cultured identify enzyme genes responsible glucosylation acylation acteoside...
Steroidal estrogens are potent endocrine-disrupting chemicals that enter natural waters through the discharge of treated and raw sewage. Because detrimental to aquatic organisms at sub-nanogram per liter concentrations, many studies have measured so-called "free" estrogen concentrations in wastewater effluents, rivers, lakes. Other forms also potential concern because conjugated can be easily converted free by bacteria treatment plants receiving halogenated likely produced during...
加熱処理を行ったイワシすり身をペプシンで加水分解し,その分解液(P-1)に含まれるACE阻害ペプチドの単離と配列決定を行って,以下の結果を得た. (1) P-1をODS充填カラムに通し,25%エタノールで溶出した画分F-3にはSP-Sephadexによるイオン交換クロマトグラフィーの結果,4つの主要な活性画分が含まれていた. (2) SP-Sephadexから0.1M蟻酸アンモニウムで二番目に溶出したB画分を逆相HPLCで分離した結果,多くのACE阻害活性を有するペプチドが含まれていた. (3) 逆相HPLCで高い活性を有していた画分をB-1, B-2, B-3としてサイズ排除クロマトグラフィーと逆相HPLCのリクロマトを行い,それぞれを単離し,以下のアミノ酸配列とIC50を有することを明らかにした.これらのペプチドは新規なACE阻害ペプチドであった. B-1: Val-Lys-Ala-Gly-Phe (IC50=83μM) B-2: Lys-Val-Leu-Ala-Gly-Met (IC50=30μM) B-3: Leu-Lys-Leu (IC50=188μM) (4)...
Red radish anthocyanin extract, which consists of 12 known acylated anthocyanins, was reacted with 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) to generate peroxyl radicals under acidic pH conditions at 37 °C. The reaction products were isolated using preparative HPLC, and their chemical structures determined be p-hydroxybenzoic acid (1), 6-O-(E)-p-coumaroyl-2-O-β-d- glucopyranosyl-α-d-glucopyranoside (3), p-coumaric (4), 6-O-(E)-feruloyl-2-O-β-d-glucopyranosyl-α-d-glucopyranoside...
Liver X receptors (LXRs) belong to the nuclear hormone receptor superfamily and function as ligand-dependent transcription factors that regulate cholesterol homeostasis, lipid immune responses. LXR antagonists are promising treatments for hypercholesterolemia diabetes. However, effective inhibitors yet be developed. Thus, we aimed develop degraders (proteolysis targeting chimeras PROTACs against LXR) a complementary strategy provide similar effect inhibition. In this study, report...
The NH2-terminal residue of a dipeptide is an important determinant the resistance to peptidases porcine small mucosa. Val or Ile, and COOH-terminal Trp Tyr dipeptides had higher angiotensin I converting enzyme(ACE)inhibitory activity digestive than other dipeptides. We defined Val-Tyr as main inhibitor in alkaline protease hydrolyzates from sardines. Attempts isolate measurement were done short chain peptides that reduced blood pressure. content was 51 mg per 100 g peptides, represented...
既存添加物のうち，酸化防止剤に分類される品目の抗酸化活性に基づく新たな品質規格策定を最終目的とし，その基礎となる抗酸化活性評価法の公定化に対する適用性の検討を行った．本研究では，代表的な分光学的抗酸化活性測定法であるDPPH法，ABTS法，WST-1法による単一化合物酸化防止剤（9種類）の抗酸化活性測定を3カ所の研究室で共同試験し，得られた結果の室間再現精度の検証を行った．その結果，DPPH法，ならびにABTS法は単一化合物酸化防止剤の抗酸化活性測定において，比較的高い測定精度を示すことが判明した．その一方で，WST-1法は先の2法と比較すると測定精度に劣るという結果が得られた．DPPH法，ならびにABTS法は更に研究室数を増やして試験室間共同試験を行い，分析法の妥当性確認を行うことにより，公定法化を進めることが可能であると考えられた．
We examined the possibility that expression of adhesion molecules is regulated differently in cultured astrocytes from stroke-prone spontaneously hypertensive rats (SHRSP/IZM) than those Wistar Kyoto (WKY/IZM) by tumor necrosis factor-alpha (TNF-alpha) or hypoxia and reoxygenation (H/R) inhibitory effects apigenin. It was found vascular cell molecule-1 (VCAM-1) TNF-alpha isolated SHRSP/IZM increased compared with WKY/IZM. The monocyte chemotactic protein-1 (MCP-1) mRNA induced H/R normal...