A5080649614- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Gene expression and cancer classification
- Radio Frequency Integrated Circuit Design
- DNA and Nucleic Acid Chemistry
- Genetically Modified Organisms Research
- Wireless Power Transfer Systems
- Advanced Power Amplifier Design
- Nitric Oxide and Endothelin Effects
- Advanced DC-DC Converters
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Plant Genetic and Mutation Studies
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Silicon Carbide Semiconductor Technologies
- NF-κB Signaling Pathways
- Energy Harvesting in Wireless Networks
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Engineering Applied Research
- Renin-Angiotensin System Studies
- Toxic Organic Pollutants Impact
National Institute of Health Sciences
2015-2025
Suzuki (Japan)
2009-2024
Tokai University
2008-2023
Hokkaido University
2002-2023
Osaka University
2022
Osaka Research Institute of Industrial Science and Technology
2020
Kyoto Prefectural University of Medicine
2019
Nagoya City University
1997-2014
Kowa (Japan)
2002-2013
Kyorin University
2009
To find effective antiprion compounds, we synthesized and evaluated various pyrazolone derivatives. Seven of 19 compounds showed inhibition PrP-res accumulation the remarkably active compound 13 an IC50 value 3 nM in both ScN2a F3 cell lines. Findings from studies on physicochemical biochemical properties suggest that action mechanism these does not correlate with any antioxidant activities, hydroxyl radical scavenging or SOD-like activities.
Nitroxyl (HNO) is a one-electron reduced and protonated derivative of nitric oxide (NO) has characteristic biological pharmacological effects distinct from those NO. However, studies its biosynthesis activities are restricted by the lack versatile HNO detection methods applicable to living cells. Here, we report first metal-free reductant-resistant imaging probe available for use in cells, P-Rhod. It consists rhodol moiety as fluorophore, linked via an ester diphenylphosphinobenzoyl group,...
The mouse has traditionally been used for the micronucleus test, with bone marrow usual target organ. aim of 9th collaborative study by CSGMT was to evaluate suitability rat and peripheral blood as Since spleen eliminates circulating micronucleated erythrocytes, a assay might not be feasible. Thirty-four Japanese laboratories six overseas participated in this collaboration, 40 chemicals were studied. As rule, analyzed using acridine orange staining. Among 36 micronucleus-positive...
A new type of photoinduced nitric oxide (NO) donors was designed from nitrobenzene derivatives. Visible-light irradiation 2,6-dimethylnitrobenzenes bearing extended pi-electron systems at the 4-position revealed efficient NO release using ESR analysis and Griess assay. Computational study ultraviolet spectrum suggested that NO-releasing activity closely related to conformation nitro group, absorption intensity, length conjugated system. Employing photodependent cytotoxicity compound 14...
Local hyperthermia has been widely used as physical therapy for a number of diseases such inflammatory osteoarticular disorders, tendinitis, and muscle injury. is clinically applied to improve blood lymphatic flow decrease swelling tissues (e.g., skeletal muscle). As repair following injury, the mechanisms underlying beneficial effects hyperthermia-induced are unknown. In this study, we investigated direct continuous heat stress on differentiation cultured mammalian myoblasts. Compared with...
The success of viral and nonviral gene delivery relies on the ability DNA-based vectors to traverse cytoplasm reach nucleus. We, as well other researchers, have shown that plasmids utilize microtubule network its associated motor proteins traffic toward While disruption microtubules with nocodazole was greatly inhibit cytoplasmic plasmid trafficking, it did not abolish it. It has been demonstrated a pool stabilized post-translationally acetylated exists in cells, this acetylation may play...
We designed and synthesized a photocontrollable peroxynitrite (ONOO–) generator, P-NAP, which has N-methyl-N-nitrosoaminophenol structure with four methyl groups introduced onto the benzene ring to block reaction of photodecomposition product ONOO– lower semiquinoneimine's redox potential. The semiquinoneimine intermediate generated by photoinduced release nitric oxide (NO) reduces dissolved molecular oxygen generate superoxide radical anion (O2•–), reacts NO afford under diffusion control...
Abstract 3‐nitrobenzanthrone (3‐NBA) is an extremely potent mutagen in the Salmonella reversion assay and a suspected human carcinogen identified diesel exhaust ambient airborne particulate matter. To evaluate vivo mutagenicity of 3‐NBA, we analyzed mutant frequency (MF) cII gene various organs (lung, liver, kidney, bladder, colon, spleen, testis) lambda/ lacZ transgenic mice (Muta Mouse) after intraperitoneal treatment with 3‐NBA (25 mg/kg body weight injected once week for 4 weeks)....
Journal Article Gene Expression Profiling of Human Mesenchymal Stem Cells for Identification Novel Markers in Early- and Late-Stage Cell Culture Get access Shihori Tanabe, Tanabe * Division Cellular Therapy Products, National Institute Health Sciences, Tokyo 158-8501, Japan *To whom correspondence should be addressed. Tel: +81-3-3700-1141, Fax: +81-3-3700-9217, E-mail: stanabe@nihs.go.jp Search other works by this author on: Oxford Academic PubMed Google Scholar Yoji Sato, Sato Takayoshi...
We synthesized hetero-Diels–Alder cycloadducts from acyl nitroso derivatives and 9,10-dimethylanthracene, to be photo-inducible HNO-releasing agents found that introduction of conjugated nitroaromatic groups effectively enhanced the responsiveness HNO release UV-A irradiation; we confirmed photoinduced formation by EPR GCMS analysis.