A5020249536- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Carcinogens and Genotoxicity Assessment
- Animal Genetics and Reproduction
- DNA Repair Mechanisms
- Sperm and Testicular Function
- Molecular Biology Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Radioactivity and Radon Measurements
- Radiation Dose and Imaging
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Effects of Radiation Exposure
- Radioactive contamination and transfer
- Genetic and phenotypic traits in livestock
- Advanced biosensing and bioanalysis techniques
- Reproductive Biology and Fertility
- RNA and protein synthesis mechanisms
- Radiation Therapy and Dosimetry
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Plant Genetic and Mutation Studies
- MicroRNA in disease regulation
- Sexual Differentiation and Disorders
- Pleistocene-Era Hominins and Archaeology
Shujitsu University
2014-2024
National Institute of Advanced Industrial Science and Technology
2001-2006
Kanagawa Cancer Center
2004
Kanagawa Prefectural Hospital Organization
2004
Itoham Foods (Japan)
1990-2001
Central Research Institute
1993-1996
Sapporo Medical University
1995-1996
Life Science Institute
1988
City Of Hope National Medical Center
1982
Nomura Research Institute
1975-1981
An expert working group on the in vivo micronucleus assay, formed as part of International Workshop Genotoxicity Test Procedures (IWGTP), discussed protocols for conduct established and proposed assays at a meeting held March 25–26, 1999 Washington, DC, conjunction with annual Environmental Mutagen Society. The reached consensus number issues, including: (1) using repeated dosing mice rats; (2) integration (rodent erythrocyte) assay into general toxicology studies; (3) possible omission...
A 2.5-kilobase fragment of a sex-specific satellite DNA from the Colubrid snake species Elaphe radiata has been cloned, and its sequence determined. It contains 26 12 copies, respectively, two base quadruplets, G-A-T-A G-A-C-A, as sole highly repetitious elements. Southern hybridization experiments with genomic chicken, mouse, man indicated male conservation at least parts this cloned DNA. In situ metaphase chromosomes mouse showed that elements can cross-hybridize are concentrated in...
Short interfering RNAs (siRNAs) are valuable reagents for sequence-specific inhibition of gene expression via the RNA interference (RNAi) pathway. Although it has been proposed that relative thermodynamic stability at 5′-ends siRNAs plays a crucial role in siRNA strand selection, we demonstrate here character 2-nt 3′-overhang is predominant determinant which participates RNAi We show with unilateral on antisense more effective than 3′-overhangs both ends, due to preferential loading into...
Ribosomes translating mRNA without an in-frame stop codon (non-stop mRNA) stall at its 3' end. In eubacteria, such ribosomes are rescued by SsrA-mediated trans-translation. Recently, we have shown that Escherichia coli ArfA (formerly YhdL) also rescues stalled a mechanism distinct from of Synthetic lethality phenotype ssrA arfA double mutants suggests accumulation is deleterious to E. cells. this report, show the expression tightly regulated system involving Both premature transcription...
The US National Academy of Sciences (NAS) presented the linear no-threshold hypothesis (LNT) in 1956, which indicates that lowest doses ionizing radiation are hazardous proportion to dose. This spurious was not based on solid data. NAS put forward BEIR VII report 2006 as evidence supporting LNT. study described used data Life Span Study (LSS) A-bomb survivors. Estimation exposure initial (5%) and neglected residual (10%), leading underestimation doses. Residual mainly consisted fallout...
<title>Abstract</title> Cells and organisms respond dynamically to environmental factors like radiation chemicals. These responses vary based on detection systems, leading terms such as adaptive response, biphasic hormesis. In micronucleus tests using cultured cells, obtaining a typical J-shaped dose-response curve, hallmark of hormesis, was challenging due low background frequency. We conducted challenge cross-challenge tests. tests, cells were pre-treated with priming doses then...
Ryukyu spiny rats (genus <i>Tokudaia</i>), which are endemic to the central part of Nansei Shoto archipelago in Japan, have unique karyotypes with odd numbers chromosomes and no cytologically recognizable Y chromosome. The chromosome <i>Tokudaia osimensis</i> from Amamioshima <i>Tokudaia</i> sp. Tokunoshima 2n = 25 45, respectively, a putative single X mouse probe hybridized unpaired chromosome, except for distal short arm female specimen <i>T. one...
Three major characteristics distinguish humans from other primates: bipedality, practical nakedness, and the family as a social unit. A hairless mutation introduced into chimpanzee/human last common ancestor (CLCA) 6 million years ago (Mya) diverged human hairy chimpanzee lineages. All primates except can carry their babies without using hands. mother would be forced to stand walk upright. Her activities markedly limited. The male partner have collect food it her by hand keep baby starving;...
ABSTRACT The A-bomb blast released a huge amount of energy: thermal radiation (35%), energy (50%), and nuclear (15%). Of the 15%, 5% was initial within 30 s 10% residual radiation, majority which fallout. Exposure doses hibakusha (A-bomb survivors) were estimated solely on basis radiation. effects have been considered controversial; some groups assert that negligible, but others refute assertion. I recently discovered six-decade-old article written in Japanese by medical doctor, Gensaku Obo,...
Abstract Ionizing radiation is regulated by the linear no-threshold model (LNT), which asserts that lowest doses of ionizing are hazardous in proportion to dose and rate. LNT based on data Life Span Study (LSS) A-bomb survivors Hiroshima Nagasaki. Radiation were estimated using initial (5% blast energy) residual (10%) was neglected. The major component fallout, most must be brought down ground black rain. rain highly radioactive. There three maps reporting covered wide areas Hiroshima-City....
The linear no-threshold model (LNT) has been the basis for radiation protection policies worldwide 60 years. LNT was fabricated without correct data. lifespan study of Atomic bomb survivors (LSS) provided fundamental data to support NLT. In LSS, exposure doses were underestimated and cancer risk overestimated; LSS do not anymore. light these findings, levels in Fukushima are reexamined. Soon after accident, International Commission on Radiological Protection issued an emergency...
We have been conducting a collaborative study on the thresholds of mutagens. In our previous examinations cell activity and proliferation as endpoints, both displayed hormesis. This time, we conducted experiments to determine using micronucleus test an endpoint.
Gene silencing with short interfering RNA (siRNA) expression vectors is a powerful method for the analysis of gene functions. For siRNA in mammalian cells, U6 small nuclear (snRNA) promoters are widely used. However, promoter might not function well other species. In this study, we cloned four putative chicken by PCR and analyzed their First, screened genomic database using human snRNA identified candidate sequences. The sequences contained some control elements regions, but as could rule...