Quercetin is a polyphenol found in food that has numerous health benefits. This study investigated the relationship between quercetin metabolism, gut microbiota composition, and dietary intake elderly Japanese subjects. A frequency questionnaire was used to assess during week prior stool sample collection. Fecal suspensions from 56 subjects were anaerobically incubated with fecal composition analyzed by next-generation sequencing. Inter-individual variations concentration at family level...

This study examined the effects of xylitol on mouse intestinal microbiota and urinary isoflavonoids. Xylitol is classified as a sugar alcohol used food additive. The seems to play an important role in isoflavone metabolism. feeding appears affect gut microbiota. We hypothesized that dietary changes and, therefore, metabolism isoflavonoids mice. Male mice were randomly divided into two groups: those fed 0.05% daidzein with 5% diet (XD group) daidzein-containing control (CD for 28 days. Plasma...

ABSTRACT: Few reports have considered the effects of dietary fiber on plasma quercetin and intestinal flora. We investigated pectin fecal flora mice fed a diet supplemented with glycoside rutin. Male were randomly divided into 2 groups, which pectin–rutin (PR) or cellulose–rutin (CR) for 14 d. Plasma isorhamnetin metabolites measured by high‐performance liquid chromatography. Feces immediately processed bacteriological procedures. The was investigated. concentrations significantly higher in...

Much attention has been focused recently on functional foods. Ume, the Japanese name for apricot of Prunus mume Sieb. et Zucc., is an example a traditional food. There are, however, few reports effects fiber from this fruit bowel function. With objective, we prepared ume to test hypothesis that it can change gut function and intestinal flora in mice. Mice were fed (UF) or cellulose (CF) diet (control) 40 days. The fecal weight, lipids, plasma lipids cecal composition microflora analyzed....

This study examined the effects of L-arabinose on mouse intestinal microbiota and urinary isoflavonoids. Male mice were randomly divided into two groups: those fed a 0.05% daidzein-2.5% diet (AR group) daidzein control (CO for 28 days. The amounts detected in urine significantly lower AR group than CO group. ratio equol/daidzein was higher (p<0.01) composition caecal flora differed between groups. occupation ratios Prevotella Lactobacillales suggests that dietary has potential to affect...

We investigated cell proliferation and apoptosis in 37 normal cycling human ovaries to determine turnover the various stages of follicular luteal development. examined by immunostaining for both Ki67 proliferative nuclear antigen (PCNA) silver staining nucleolar organizer regions (AgNORs). Apoptosis was 3'-hydroxy nick-end labeling an apoptosis-related antigen, Ley. The indexes Ki67, PCNA, AgNORs were significantly increased antral follicles. However, there no significant differences index...

Germfree mice died when they were fed a purified diet of AIN-76 formula sterilized by gamma-irradiation. Vitamin K deficiency was suspected and this study performed to confirm the cause death. diets or AIN-93M formula, which pelleted gamma-irradiation at dose 50 kGy. One half within two weeks surviving animals also in poor health, while 91% survived. No hemorrhage observed grossly any organs animals. Histologically, degeneration with inflammatory cell infiltration as well fibrosis heart...

This study examined the effects of rice bran oil (RBO) on mouse intestinal microbiota and urinary isoflavonoids. Dietary RBO affects cholesterol absorption. Intestinal seem to play an important role in isoflavone metabolism. We hypothesized that dietary changes metabolism isoflavonoids mice. Male mice were randomly divided into two groups: those fed a 0.05% daidzein with 10% diet (RO group) lard control (LO for 30 days. Urinary amounts dihydrodaidzein significantly lower RO group than LO...

A rod-shaped and Gram-positive anaerobic S-equol-producing intestinal bacterium, TM-30, was isolated from the feces of a healthy human. The 16S rRNA partial sequence strain TM-30 exhibited 99% identity to that Slackia sp. NATTS. produced S-equol daidzein. in vitro incubation β-estradiol with yielded estrone, estrone β-estradiol. producing activity higher than activity. results presented herein demonstrate human likely plays role metabolism vitro.

The effects of Lactobacillus gasseri JCM 1131(T) on isoflavonoid levels within the caecum and plasma were assessed in adult mice. Male 5-week-old mice fed an AIN 93M diet for 30 d. Two groups administered either L. (the LGI group) or physiological saline solution control (CI) daily 5 d before dissection. daidzein concentration was significantly higher group, however, their equol less than CI group. total amount present as aglycone greater but there no significant difference caecal aglycone....

Japanese apricot (Prunus mume Siebold & zucc.) fruits of 'Nanko' have softer flesh than those 'Gojiro'. Therefore, there are differences in the processability these cultivars. We investigated characteristics cell-wall polysaccharide degradation that significantly affects fruit firmness 2 cultivars at unripe, ripe, and drop stages. Fruit 'Gojiro' was maintained during unripe ripe stages decreased after stage, while constantly lower Amount pectin hemicellulose substances commonly process...

Natto is a major functional food in Japan. γ-Polyglutamic acid (γ-PGA) one of the main components Natto, and beneficial effects γ-PGA have been reported. We tested hypothesis that alleviates metabolic syndrome KK-Ay/TaJcl male mice. These mice were fed diet containing 0.5% or control for 28 days. Visceral fat was significantly smaller PGA group than CON group. The amounts lipids feces (dry weight) sampled on final day experiment cecal Lactobacillus counts greater Cecal Prevotella tended to...

Natto is a traditional Japanese fermented soy product high in γ-polyglutamic acid (γ-PGA), whose beneficial effects have been reported. We prepared high-γ-PGA natto and compared the dietary influence on liver lipids cecal microbiota mice fed diet containing it or standard diet. The were served 30% (PGA group) (Con for 28 days. Liver lipids, fecal bile acids quantified. Cecal analyzed by PCR amplification of V3 V4 regions 16S rRNA genes sequenced using MiSeq System. Additionally, short-chain...

Protective effect of quercetin on high-fat diet-induced non-alcoholic fatty liver disease in mice has been reported. Recent research revealed that several intestinal bacteria metabolize quercetin. We hypothesize the difference composition microbiota affects absorption from intestine. Germ-free BALB/cA female (18 weeks old) were randomly divided into four groups and orally administered with fecal suspension human individuals (HF1, HF2, HF3, HF4) to produce microbiota-associated mice. All fed...

Equol is a metabolite of daidzein that produced by intestinal microbiota. The oestrogenic activity equol stronger than daidzein. Equol-producing bacteria are believed to play an important role in the gut. rod-shaped and Gram-positive anaerobic equol-producing bacterium Slackia TM-30 was isolated from healthy human faeces its effects on urinary phyto-oestrogen, plasma faecal lipids were assessed adult mice.The amounts urine significantly higher mice receiving (BAC) group control (CO) (P <...

Much attention has focused on the health benefits of isoflavone. Investigating absorption and metabolism isoflavonoids is essential for understanding their biological activity. We explored effect a high fiber diet containing rice bran hemicellulose plasma cecal in mice. Mice were fed 5% cellulose-0.1% daidzein (RBI diet) or (CI 30 days. After 30-day feeding period, mice sacrificed blood contents collected. The total cholesterol concentrations measured. significantly lower RBI group compared...

Much attention has been focused on the biological effects of equol, a metabolite daidzein produced by intestinal microbiota. However, little is known about role isoflavone metabolizing bacteria in Recently, we isolated dihydrodaidzein (DHD)-producing Clostridium-like bacterium, strain TM-40, from human feces. We investigated TM-40 vitro metabolism fecal microbiota male equol producer and two non-producers. In suspension non-producer DHD producer, was detected incubation after addition TM-40....

Much attention has been focused on the potential health-promoting effects of quercetin, a flavonoid that is metabolized by intestinal microbiota. However, there are few reports bacteria degrade quercetin. We isolated quercetin-degrading bacterium strain 19 – 20 (Accession no: LC175305) from human feces. Based comparative 16S rRNA gene sequence analysis, exhibits 100% similarity to Clostridium orbiscindens Y18187). Quercetin degradation was observed increase in time-dependent manner when...