A5011061962- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Bacterial Infections and Vaccines
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Poxvirus research and outbreaks
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Viral Infectious Diseases and Gene Expression in Insects
- Liver Disease and Transplantation
- Hepatitis C virus research
- Viral-associated cancers and disorders
- Polyomavirus and related diseases
- Full-Duplex Wireless Communications
- Veterinary medicine and infectious diseases
- Viral Infections and Vectors
National Institute of Infectious Diseases
2015-2024
Toshima Hospital
2020
Murayama Medical Center
2019
The Nakamura Hajime Eastern Institute
2017
Osaka University
2017
Microbial Chemistry Research Foundation
2017
National Institutes of Health
2001-2015
Musashi University
2014
Abstract The spike (S) protein of coronavirus, which binds to cellular receptors and mediates membrane fusion for cell entry, is a candidate vaccine target blocking coronavirus infection. However, some animal studies have suggested that inadequate immunization against severe acute respiratory syndrome (SARS‐CoV) induces lung eosinophilic immunopathology upon present study evaluated two kinds adjuvants use with recombinant S protein: gold nanoparticles (AuNPs), are expected function as both...
Abstract An autopsy of a patient in Japan with coronavirus disease indicated pneumonia lung pathology, manifested as diffuse alveolar damage. We detected severe acute respiratory syndrome 2 antigen epithelial cells and macrophages. Coronavirus is essentially lower tract characterized by direct viral injury cells.
Abstract Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic caused by the SFTS phlebovirus (SFTSV). patients were first reported in China, followed Japan and South Korea. In 2017, cats diagnosed for time, suggesting that these animals are susceptible to SFTSV. To confirm whether or not indeed SFTSV, animal subjects experimentally infected Four of six SPL010 strain SFTSV died, all showing similar more severe symptoms than human patients, such as a fever,...
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic caused by a tick-borne banyangvirus and associated high fatality. Despite increasing incidence of SFTS serious public health concerns in East Asia, the pathogenesis lethal virus (SFTSV) infection humans not fully understood. Numbers postmortem examinations to determine target cells viral have so far been limited. Here we showed that B differentiating into plasmablasts macrophages secondary lymphoid organs were...
One safety concern during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development has been the vaccine-associated enhanced disease, which is characterized by eosinophilic immunopathology and T helper cell type (T
Hepatitis E virus (HEV) is a causative agent of hepatitis E. Recently, novel E-like was isolated from Norway rats in Germany. However, the antigenicity, pathogenicity and epidemiology this are unclear because lack cell-culture system which to grow it. In study, an N-terminally truncated ORF2 protein expressed insect Tn5 cells using recombinant baculovirus expression large amount 53 kDa efficiently released into supernatant. Electron microscopic analyses purified revealed that self-assembled...
Brucella species replicate within host cells in the form of endoplasmic reticulum (ER)-derived vacuoles. The mechanisms by which bacteria are sequestered into such vacuoles and obtain a continuous membrane supply for their replication remain to be elucidated. In present study, we provided several lines evidence that demonstrate mechanism B. abortus acquires ER-derived membrane. First, during infection, IRE1 pathway, but not PERK ATF6 pathways, unfolded protein response (UPR) was activated...
Mpox virus (MPXV) is a zoonotic orthopoxvirus and caused an outbreak in 2022. Although tecovirimat brincidofovir are approved as anti-smallpox drugs, their effects mpox patients have not been well documented. In this study, by drug repurposing approach, we identified potential candidates for treating predicted clinical impacts mathematical modeling.
We report the pathological and virological findings of first autopsy case 2009 pandemic influenza (A/H1N1pdm) virus infection in Japan. A man aged 33 years with chronic heart failure due to dilated cardiomyopathy, mild diabetes mellitus, atopic dermatitis, bronchial asthma, obesity died respiratory multiple organ dysfunction syndrome. Macroscopic examination showed severe pulmonary edema microscopically lung sections very early exudative-stage diffuse alveolar damage (DAD)....
Exosomes are small vesicles released from cells, into which microRNAs (miRNA) specifically sorted and accumulated. Two gamma-herpesviruses, Kaposi sarcoma-associated herpesvirus (KSHV) Epstein-Barr virus (EBV), encode miRNAs in their genomes express virus-encoded cells exosomes. However, there is little information about the detailed distribution of In this study, we thus identified virus- host-encoded exosomes KSHV- or EBV-infected lymphoma cell lines compared them with intracellular using...
Protocols for chemical synthesis and in vitro assembly of the hepatitis B virus full-length core protein (Cp183) were investigated.
A fusion protein expressed on the surface of enveloped viruses mediates viral and cellular membranes to facilitate virus infection. Pre- postfusion structures proteins have been characterized, but conformational changes between them remain poorly understood. Here, we examined intermediate conformation murine coronavirus protein, called spike which must be cleaved by a protease following receptor binding. Western blot analysis digestion products revealed that two subunits (67 69 kDa) are...
Significance Human sapoviruses (HuSaV) are an important cause of diarrhea and foodborne infections worldwide. Despite the discovery HuSaVs over 40 y ago, no in vitro cell culture system has been established, limiting research on this pathogen. In study, we report successful propagation HuSaV a human duodenum line presence bile acids. Bile acids biological components intestinal tract, they recapitulate key physiologic factors present viral infection site intestine. This inexpensive,...
The development of effective antiviral drugs targeting the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is urgently needed to combat disease 2019 (COVID-19). We have previously studied use semi-synthetic derivatives oxysterols, oxidized cholesterol as drug candidates for inhibition cancer, fibrosis, and bone regeneration. In this study, we screened a panel naturally occurring oxysterols anti-SARS-CoV-2 activity using cell culture infection assay. show that natural...
The GP129, GP131 and GP133 genes of guinea pig cytomegalovirus (GPCMV) are homologues human UL128, UL130 UL131A, respectively, which essential for infection endothelial epithelial cells, viral transmission to leukocytes. Our previous study demonstrated that a GPCMV strain lacking the 1.6 kb locus contains had growth defect in animals. Here, we WT strain, but not kb-deleted formed capsids macrophages prepared from peritoneal fluid. To understand mechanism, strains defective each GP133, found...
Mpox virus (formerly monkeypox [MPXV]) is a neglected zoonotic pathogen that caused worldwide outbreak in May 2022. Given the lack of an established therapy, development anti-MPXV strategy vital importance. To identify drug targets for agents, we screened chemical library using MPXV infection cell assay and found gemcitabine, trifluridine, mycophenolic acid (MPA) inhibited propagation. These compounds showed broad-spectrum anti-orthopoxvirus activities presented lower 90% inhibitory...
Porcine sapelovirus (PSV) is a causative agent of neurological disorders, fertility disorders and dermal lesions swine. In this study, we isolated two PSV strains, Jpsv477 Jpsv1315, from swine faecal specimens using PLC/PRF/5 cell culture system. The infection cells induced cytopathic effect (CPE). Two types virus particles with identical diameter (~35 nm) but different densities (1.300 1.285 g/cm3 ) were observed in the supernatants. Analysis entire genome sequence Jpsv1315 revealed that...
ABSTRACT Cell culture systems reproducing virus replication can serve as unique models for the discovery of novel bioactive molecules. Here, using a hepatitis C (HCV) cell system, we identified neoechinulin B (NeoB), fungus-derived compound, an inhibitor liver X receptor (LXR). NeoB was initially by chemical screening compound that impeded production infectious HCV. Genome-wide transcriptome analysis and reporter assays revealed specifically inhibits LXR-mediated transcription. also shown to...
Infection of Epstein–Barr virus (EBV), a ubiquitous human gamma herpesvirus, is associated with various malignancies in B lymphocytes and epithelial cells. EBV encodes 49 microRNAs two separated regions, termed the BART BHRF1 loci. Although accumulating evidence demonstrates that infection regulates profile cells, little known about exosomes released from infected Here, we characterized expression intracellular exosomal EBV-negative, related EBV-infected Burkitt lymphoma cell lines having...
We devised a reverse genetics system to generate an infectious human sapovirus (HuSaV) GII.3 virus. Capped/uncapped full-length RNAs derived from HuSaV AK11 strain generated by in vitro transcription were used transfect HuTu80 duodenum carcinoma cells; viruses recovered the capped RNA-transfected cells and passaged cells. Genome-wide analyses indicated no nucleotide sequence change virus genomes cell-culture supernatants transfection or those subsequent infection. No growth was detected...
Abstract Monkeypox virus (MPXV) is a zoonotic orthopoxvirus that causes smallpox-like symptoms in humans and caused an outbreak May 2022 led the WHO to declare global health emergency. In this study, from screening of approved-drug libraries using MPXV infection cell system, atovaquone, mefloquine, molnupiravir exhibited anti-MPXV activity, with 50% inhibitory concentrations 0.51-5.2 μM, which more potent than cidofovir. Whereas mefloquine was suggested inhibit viral entry, atovaquone...
Obesity has been recognized as one of the most significant risk factors for deterioration and mortality associated with COVID-19, but significance obesity itself differs among ethnicity. Multifactored analysis our single institute–based retrospective cohort revealed that high visceral adipose tissue (VAT) burden, not other obesity-associated markers, was related to accelerated inflammatory responses Japanese COVID-19 patients. To elucidate mechanisms how VAT-dominant induces severe...