A5106631591- Pelvic floor disorders treatments
- Anorectal Disease Treatments and Outcomes
- Diverticular Disease and Complications
- Veterinary Oncology Research
- Colorectal Cancer Surgical Treatments
- Gastrointestinal disorders and treatments
- Congenital gastrointestinal and neural anomalies
- Hernia repair and management
- Pancreatic and Hepatic Oncology Research
- Central Venous Catheters and Hemodialysis
- Helicobacter pylori-related gastroenterology studies
- Nitric Oxide and Endothelin Effects
- Infectious Diseases and Mycology
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Mast cells and histamine
- Telomeres, Telomerase, and Senescence
- Infective Endocarditis Diagnosis and Management
- Hedgehog Signaling Pathway Studies
- Immunotherapy and Immune Responses
- Regulation of Appetite and Obesity
- Neurobiology and Insect Physiology Research
- Pulmonary Hypertension Research and Treatments
- Gastric Cancer Management and Outcomes
- Monoclonal and Polyclonal Antibodies Research
Kameda Medical Center
2015-2024
Kobe University Hospital
2019-2024
Nihon University
2004-2023
Kanagawa Cancer Center
2021-2023
Kanagawa Prefectural Hospital Organization
2021-2023
Kameda College of Health Sciences
2023
Tomishiro Central Hospital
2023
Iwate Medical University
2019-2021
Oita Medical Center
2021
Hokkaido University
2005-2021
Hydrogen sulfide (H(2)S), an endogenous gasotransmitter, modulates various biological events such as inflammation in the mammalian body. The present study investigated possible involvement of H(2)S peripheral nociceptive processing. Intraplantar (i.pl.) administration NaHS, a donor, produced prompt hyperalgesia rats, accompanied by expression Fos spinal dorsal horn. H(2)S-evoked was blocked 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB), oxidizing agent, or ethosuximide and mibefradil, T-type...
Marked sarcomere disorganization is a well-documented characteristic of cardiomyocytes in the failing human myocardium. Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v), which involved development cardiomyopathy, an important structural protein that affects physiologic cardiac formation and heart development. Integrated cDNA expression analysis myocardia uncovered novel kinase, cardiac-specific myosin kinase (cardiac-MLCK), acts on MLC2v. Expression levels...
<h3>Objective:</h3> Hydrogen sulfide (H<sub>2</sub>S) is formed from l-cysteine by multiple enzymes including cystathionine-γ-lyase (CSE) in mammals, and plays various roles health disease. Recently, a pronociceptive role for H<sub>2</sub>S the processing of somatic pain was identified. Here, involvement pancreatic examined. <h3>Methods:</h3> Anaesthetised rats or mice received an injection NaHS, donor H<sub>2</sub>S, capsaicin into duct, expression spinal Fos protein detected...
Abstract Objective —To characterize the clinical features of visceral mast cell tumors (MCT) without associated cutaneous involvement in dogs. Design —Retrospective study. Animals —10 dogs with histologically confirmed MCT lesions. Procedure —Information on signalment, signs, laboratory examinations, and time from first admission to death was obtained medical record each dog. Results —Purebred male miniature breeds appeared have a higher prevalence MCT. Clinical signs included anorexia,...
There are many orphan G-protein-coupled receptors (GPCRs) for which ligands have not yet been identified. One such GPCR is the bombesin receptor subtype 3 (BRS-3). BRS-3 plays a role in onset of diabetes and obesity. GPCRs invertebrates similar to those vertebrates. Two Drosophila (CG30106 CG14593) belong phylogenetic subgroup. Here, we succeeded biochemically purify endogenous CG30106 CG14593 from whole homogenates using functional assays with reverse pharmacological technique, identified...
Journal Article Production of n-octanoyl-modified Ghrelin in Cultured Cells Requires Prohormone Processing Protease and O-acyltransferase, as well n-octanoic Acid Get access Tomoko Takahashi, Takahashi 1Molecular Genetics, Institute Life Science, Kurume University, Kurume, Fukuoka 839-0864; 2Department Biological Science Technology, Faculty Engineering, University Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan Search for other works by this author on: Oxford Academic PubMed Google...
Abstract Pancreatic cancer frequently involves cancer‐associated thromboembolism, which is strongly associated with poor prognosis. Tissue factor, a blood coagulation factor largely produced in patients as component of extracellular vesicles, plays key role the incidence thromboembolism pancreatic cancer. However, no prospective studies have been published on relationship between tissue and or patient clinical characteristics, including recent chemotherapy regimens. Thus, we aimed to address...
Significance Human sapoviruses (HuSaV) are an important cause of diarrhea and foodborne infections worldwide. Despite the discovery HuSaVs over 40 y ago, no in vitro cell culture system has been established, limiting research on this pathogen. In study, we report successful propagation HuSaV a human duodenum line presence bile acids. Bile acids biological components intestinal tract, they recapitulate key physiologic factors present viral infection site intestine. This inexpensive,...
Abstract We investigated whether early circulating tumor DNA (ctDNA) changes, measured using digital PCR (dPCR), can predict later chemotherapy responses in esophageal squamous cell cancer (ESCC). compared the dynamics of ctDNA and volumes during 42 ESCC. The accuracy predictions was evaluated by ratio variant allele frequency (post-/pre-ctDNA) total volume (post-/pre-volume) before after an initial cycle a receiver-operating characteristic curve analysis. Total positive negative objective...
To clarify the mechanism of leukemogenesis in atomic bomb survivors, leukemic cells were investigated using fluorescence situ hybridization (FISH) analysis on basis conventional G-banding patients with a history radiation exposure and also de novo patients. Conventional showed higher incidences (p< 0.005) structural numerical abnormalities without any specific types chromosome aberrations group exposed to dose more than one Gy, compared non-exposed group. FISH revealed significantly (P<...
Introduction Although long‐term crude outcomes of laparoscopic ventral rectopexy for external rectal prolapse (ERP) have been documented, repetitive functional and quality life (QOL) assessments are scarce. This study assessed midterm annual results QOL after ERP. Methods consisted 58 patients was a retrospective analysis prospectively collected data. The Fecal Incontinence Severity Index, the Constipation Scoring System, instruments (ie 36‐item Short‐Form Health Survey Quality Life scale)...
Tissue factor pathway inhibitor‑2 (TFPI‑2) is a promising candidate as serum biomarker of ovarian clear cell carcinoma (OCCC), lethal histological subtype epithelial cancer (EOC). TFPI‑2 secreted serine protease inhibitor that suppresses progression through the inhibition matrix activities. Previous studies have also identified in nucleus, and possible function nuclear transcriptional repressor metalloproteinase‑2 (MMP‑2) was recently demonstrated. We are currently establishing for OCCC...
Dresden G protein-coupled receptor (D-GPCR) is one of orphan receptors (GPCR). Here we report the identification ligands and characterization D-GPCR. We investigated over 5000 compounds to evoke response mediated by D-GPCR identified 3-methyl-valeric acid 4-methyl-valeric as agonists using a cAMP assay. It interest that they dramatically enhanced intracellular accumulation CRE-luciferase activity in CHO-K1 cells HEK293 expressing chimeric protein with rhodopsin-tag at its N-terminus. Our...
This study evaluated continence, constipation and quality of life (QOL) before after laparoscopic ventral rectopexy (LVR) METHOD: Between February 2012 July 2014, patients who underwent LVR for external rectal prolapse (ERP) and/or rectoanal intussusception (RAI) were prospectively included. A standard questionnaire including the Fecal Incontinence Severity Index (FISI), Constipation Scoring System (CSS) QOL instruments (Short-Form 36 Health Survey, scale, Patient Assessment...
PURPOSE: Because of the rarity diagnosis, clinical implication enterocele remains unclear. This study was designed to clarify characteristic symptoms and role surgical treatment for enterocele. METHODS: We reviewed a defecography database prospectively maintained from 1998 2004. Defecography performed with oral barium opacify small bowel in all patients. Enterocele defined as falling down beyond level vaginal vault practically, it <3 cm anorectal angle lateral view. Patients' information...
This study was designed to evaluate the clinical and physiologic outcomes after transvaginal rectocele repair.Between June 2000 January 2003, 30 females (mean age, 62 (range, 45-78) years) with a symptomatic large (>3 cm) underwent repair (anterior levatorplasty). Six months surgery, evaluation performed by using defecography (depth of rectocele) anorectal manometry (maximum resting pressure, maximum squeeze rectal threshold, tolerable volume). Using questionnaire, one year surgery analyze...
We evaluated the usefulness of polymerase chain reaction for antigen receptor gene rearrangement analysis (PARR) endoscopic biopsy specimens diagnosis canine alimentary lymphoma. Two were obtained from each lesion in 78 dogs with gastrointestinal symptoms. One specimen was histopathologically examined by a pathologist, and other analyzed PARR. All samples categorized into three groups [lymphoma (n=4), adenocarcinoma (n=5) enteritis (n=69)] based on histopathological diagnosis. In lymphoma...
Royal jelly (RJ), a creamy substance secreted by honeybees, is the exclusive diet for queen bee differentiation and life maintenance. RJ has been used in cosmetics, beverages, medicines, supplements worldwide. However, allergy concerning issue RJ, especially atopic dermatitis (AD) asthma patients. In some cases, allergic reactions are seen after first intake of suggesting existence allergens cross-reactive with RJ. Information about very important safe application RJ; however, study this...
Cancer tissues generally have molecular oxygen and serum component deficiencies because of poor vascularization. Recently, we revealed that ICAM1 is strongly activated through lipophagy in ovarian clear cell carcinoma (CCC) cells response to starvation long-chain fatty acids confers resistance apoptosis caused by these harsh conditions. CD69 a glycoprotein synthesized immune associated with their activation cellular signaling pathways. However, the expression function nonhematological...
The inhibitory effect of glucocorticoids (GCs) on proliferation canine mast cell tumor (MCT) was studied using two types MCT cells; JuMC cells and LuMC derived from spontaneous cutaneous intestinal MCT, respectively. In in vitro study, growth significantly inhibited with more than 1 nM GCs apoptotic-like death seen, while that never even 10 μM GCs. Growth rate masses nude mice developed by inoculation reduced a dose-dependent manner administration GC, inhibition minimal increasing GC doses....
<i>Background:</i> Recent data suggest that normal tissue mast cells can express functional receptors for IgG under certain conditions. However, little is known about receptor expression and consequences in cell neoplasms. <i>Methods:</i> In this study, neoplastic were obtained from a dog with cutaneous mastocytoma (CM-MC) visceral (VI-MC). Both populations characterized morphologically functionally. <i>Results:</i> Most proliferated constantly suspension...