A5059295432- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Cellular transport and secretion
- Pharmacological Effects of Natural Compounds
- Studies on Chitinases and Chitosanases
- Ubiquitin and proteasome pathways
- Microbial Metabolic Engineering and Bioproduction
- Acute Myeloid Leukemia Research
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Chronic Lymphocytic Leukemia Research
- Traditional Chinese Medicine Studies
- Plant-based Medicinal Research
- Mycobacterium research and diagnosis
- Pathogenesis and Treatment of Hiccups
- Food Quality and Safety Studies
- Genomic variations and chromosomal abnormalities
- Gout, Hyperuricemia, Uric Acid
- Microbial Natural Products and Biosynthesis
- Pharmacy and Medical Practices
- Parkinson's Disease Mechanisms and Treatments
- Peptidase Inhibition and Analysis
Tohoku University
2013-2024
Kobe Tokiwa University
2017-2019
Kobe University
2002-2017
RMIT University
2010-2014
Hiroshima University
1975-2012
Kindai University
2003-2011
University of Michigan
2002-2005
Research Institute for Bioscience and Biotechnology
2005
Chiba University
2004
Tohoku Medical and Pharmaceutical University
1988-2004
Autophagy is a membrane trafficking to vacuole/lysosome induced by nutrient starvation. In Saccharomyces cerevisiae, Tor protein, phosphatidylinositol kinase-related kinase, involved in the repression of autophagy induction largely unknown mechanism. Here, we show that protein kinase activity Apg1 enhanced starvation or rapamycin treatment. addition, have also found Apg13, which binds and activates Apg1, hyperphosphorylated Tor-dependent manner, reducing its affinity Apg1. This Apg1–Apg13...
Selective incorporation of cargo proteins into the forming vesicle is an important aspect protein targeting via vesicular trafficking. Based on current paradigm selection in transport, to be sorted other organelles are condensed at budding site donor organelle, a process that mediated by interaction between and coat proteins, which constitute part machinery. The cytoplasm vacuole (Cvt) pathway unconventional trafficking yeast, topologically mechanistically related autophagy. Aminopeptidase I...
Autophagy is a degradative process conserved among eukaryotic cells. It allows the elimination of cytoplasm including aberrant protein aggregates and damaged organelles. Accordingly, it implicated in normal developmental processes also serves protective role tumor suppression invading pathogens, whereas defects autophagy are associated with various human diseases cancer neurodegeneration. Atg proteins mediate sequestration event that occurs at preautophagosomal structure (PAS) by catalyzing...
Macroautophagy is a catabolic pathway used for the turnover of long-lived proteins and organelles in eukaryotic cells. The morphological hallmark this process formation double-membrane autophagosomes that sequester cytoplasm. Autophagosome most complex part macroautophagy, it dynamic event likely involves vesicle fusion to expand initial sequestering membrane, phagophore; however, essentially nothing known about including molecular components involved tethering fusion. In study, we provide...
Successful treatment of aspergillosis caused by Aspergillus fumigatus is threatened an increasing incidence drug resistance. This situation further complicated the finding that strains resistant to azoles, major antifungal drugs for aspergillosis, have been widely disseminated across globe. To elucidate mechanisms underlying azole resistance, we identified a novel transcription factor required normal resistance in fungi including A. fumigatus, oryzae, and nidulans. fungal-specific Zn2-Cys6...
Autophagy is a catabolic multitask transport route that takes place in all eukaryotic cells. During starvation, cytoplasmic components are randomly sequestered into huge double-membrane vesicles called autophagosomes and delivered the lysosome/vacuole where they destroyed. Cells able to modulate autophagy response their needs, under certain circumstances, cargoes such as aberrant protein aggregates, organelles bacteria can be selectively exclusively incorporated autophagosomes. In yeast...
The Cvt pathway is a biosynthetic transport route for distinct subset of resident yeast vacuolar hydrolases, whereas macroautophagy nonspecific degradative mechanism that allows cell survival during starvation. Yet, these two trafficking pathways share number identical molecular components and are morphologically very similar. For example, one the hallmarks both formation double-membrane cytosolic vesicles sequester cargo before delivery. origin vesicle membrane has been controversial...
Macroautophagy is a catabolic process by which cytosolic components are sequestered double membrane vesicles called autophagosomes and sorted to the lysosomes/vacuoles be degraded. Saccharomyces cerevisiae has adapted this mechanism for constitutive transport of specific vacuolar hydrolases aminopeptidase I (Ape1) α-mannosidase (Ams1); cytoplasm vacuole targeting (Cvt) pathway. The precursor form Ape1 self-assembles into an aggregate-like structure in cytosol that then recognized Atg19...
Autophagy is a degradative process in which cytoplasmic components are non-selectively sequestered by double-membrane structures, termed autophagosomes, and transported to the vacuole. We have identified characterized novel protein Apg2p essential for autophagy yeast. Biochemical fluorescence microscopic analyses indicate that functions at step of autophagosome formation. localizes some membranous structure distinct from any known organelle. Using fluorescent protein-tagged Apg2p, we showed...
Hochu-ekki-to is a traditional herbal (Kampo) medicine that has been shown to be effective for patients with Kikyo (delicate, easily fatigable, or hypersensitive) constitution. Previous case reports have suggested this drug was certain subgroup of atopic dermatitis (AD). We aimed evaluate the efficacy and safety in long-term management AD. In multicenter, double blind, randomized, placebo-controlled study, 91 AD were enrolled. condition evaluated by questionnaire scoring system. All...
Since the atomic bomb explosions in Hiroshima and Nagasaki, high incidences of leukemia, thyroid cancer other tumors have been reported as bomb-induced tumors. We investigated incidence meningioma among survivors. Sixty-eight patients surgically treated for who had within 2.0 km hypocenter explosion were identified. Six hundred seven non-exposed with also studied. Treatment dates from 1975 to 1992.The 68 subjects 607 8.7 3.0 cases per 105 persons year, respectively. The survivors 5-year...
We investigated the effect of codon optimization on expression levels heterologous proteins in Aspergillus oryzae, using mite allergen Der f 7 as a model protein. A codon-optimized gene was synthesized according to frequency usage A. oryzae by recursive PCR. Both native and optimized genes were expressed under control high-level-expression promoter with their own signal peptides or fusion construct glucoamylase (GlaA). Codon markedly increased protein mRNA production both nonfused GlaA-fused...
To clarify the mechanism of leukemogenesis in atomic bomb survivors, leukemic cells were investigated using fluorescence situ hybridization (FISH) analysis on basis conventional G-banding patients with a history radiation exposure and also de novo patients. Conventional showed higher incidences (p< 0.005) structural numerical abnormalities without any specific types chromosome aberrations group exposed to dose more than one Gy, compared non-exposed group. FISH revealed significantly (P<...