A5062119870- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- S100 Proteins and Annexins
- Insect and Pesticide Research
- Viral Infections and Vectors
- Blood Coagulation and Thrombosis Mechanisms
- Mosquito-borne diseases and control
- Autoimmune and Inflammatory Disorders Research
- Parasites and Host Interactions
- Toxoplasma gondii Research Studies
- Rabies epidemiology and control
- T-cell and B-cell Immunology
- Malaria Research and Control
- Immune Response and Inflammation
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Insect Pest Control Strategies
- Macrophage Migration Inhibitory Factor
- Fossil Insects in Amber
- Human Health and Disease
- Galectins and Cancer Biology
- Paraoxonase enzyme and polymorphisms
- Pancreatitis Pathology and Treatment
- Dermatological diseases and infestations
- Dengue and Mosquito Control Research
The University of Tokyo
2016-2025
University of Niigata Prefecture
2022
Ege University
2016
Visceral leishmaniasis (VL) is the most severe form of and fatal if left untreated in over 95% cases. Leishmaniasis one neglected tropical diseases that tend to thrive developing regions world where inadequate access healthcare makes it difficult for some people even receive a diagnosis. This study examined usefulness oral swabs as specimens VL diagnosis, by detecting Leishmania donovani DNA from both patients L. donovani-infected mice. Eighty swab (OS) blood buffy coat (BC) samples were...
Visceral leishmaniasis (VL) is the most severe form of caused by protozoan parasites genus Leishmania. Hepatomegaly one frequent clinical manifestations VL, whereas immunopathology symptom has not been well investigated. Using our chronic model experimental we examined influence Leishmania donovani infection on liver clinical, histological, and biochemical analyses. The infected mice showed increased weight 24 weeks post-infection. Although an increase in serum ALT inflammatory cell...
Abstract Background In Central Asian foci of zoonotic cutaneous leishmaniases, mixed infections Leishmania turanica and L. major have been found in a reservoir host (the great gerbil, Rhombomys opimus ) as well the sand fly vector Phlebotomus papatasi , but hybrids between these two species never reported. addition, role other than P. circulation is not clear. Methods this work we compared development three belonging to different subgenera. studied experimental co-infections flies by both...
Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated remained elusive due to lack appropriate animal models. Here, we have established mouse model VL hemophagocytosis. At 24 weeks after infection 1 x 107 Leishmania donovani promastigotes, BALB/cA mice...
Human indigenous cutaneous leishmaniasis caused by Leishmania donovani complex is endemic in Sri Lanka. We performed an entomological survey to determine the distribution of probable vector species. Sand flies were collected districts dry zone, wet zone highlands, and coastal belt Lanka using CDC light traps, sticky traps cattle-baited net during July, 2005. The was reconducted February, 2006. Overall, 584 sand belonging Phlebotomus (266 specimens, 2 species) Sergentomyia (318 8 genera...
Visceral leishmaniasis (VL) is caused by parasitic protozoa of the genus Leishmania and characterized clinical manifestations such as fever, hepatosplenomegaly anemia. Hemophagocytosis, phenomenon phagocytosis blood cells macrophages, found in VL patients. In a previous study we established an experimental model VL, reproducing anemia mice for first time, identified hemophagocytosis heavily infected macrophages spleen possible cause However, mechanism parasite-induced or its role parasite...
Canine distemper virus (CDV) vaccination confers long-term protection against CDV reinfection. To investigate the utility of as a polyvalent vaccine vector for Leishmania, we generated recombinant CDVs, based on an avirulent Yanaka strain, that expressed Leishmania antigens: LACK, TSA, or LmSTI1 (rCDV–LACK, rCDV–TSA, and rCDV–LmSTI1, respectively). Dogs immunized with rCDV-LACK were protected challenge lethal doses virulent CDV, in same way parental strain. evaluate protective effects CDVs...
Elevation of serum B-cell activating factor (BAFF) is one the characteristics immunological disorders, including autoimmunity, but levels BAFF in infectious diseases have not been studied well. Here, we showed elevation patients with visceral leishmaniasis (VL). The mean value VL (4.65 ng/mL) was 4.3 times higher than that healthy controls (1.08 ng/mL), and 90% above cutoff calculated as + 3 SDs controls. This report first on during VL.
Leishmaniasis is caused by infection with protozoan parasites of the genus Leishmania. In both clinical and experimental visceral leishmaniasis, macrophage multinucleation observed in parasitized tissues. However, feature mechanism remained unclear. Here, we report that Leishmania donovani, a causative agent induces bone marrow-derived macrophages (BMDMs) vitro. When these infection-induced multinucleated were compared cytokine-induced giant cells, former had higher phagocytic activity on...
One of the most characteristic clinical features in cutaneous leishmaniasis is development nodules followed by ulcerations at site infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In present study, roles T cells ulceration were examined using SCID cell reconstitution experiments. After nodules, inoculated with splenocytes from either or...
Visceral leishmaniasis (VL) is an infectious disease caused by parasitic protozoa of the genus Leishmania and manifests clinical symptoms such as splenomegaly, hepatomegaly, anemia, fever. It has previously been shown that B-cell-activating factor (BAFF) involved in splenomegaly during VL. Although BAFF known to be expressed a variety cells, mechanism elevated expression VL not clear. In this study, we aimed identify BAFF-producing cells spleens mice infected with donovani. Splenocytes L....
IL-25, IL-33 and TSLP, which are produced predominantly by epithelial cells, can induce production of Th2-type cytokines such as IL-4, IL-5 and/or IL-13 various types suggesting their involvement in induction cytokine-associated immune responses. It is known that contribute to host defense against malaria parasite infection mice. However, the roles TSLP remain unclear. Thus, elucidate this, we infected wild-type, IL-25-/-, IL-33-/- receptor (TSLPR)-/- mice with Plasmodium berghei (P....
The shedding of cell surface receptors may bring synergistic outcomes through the loss receptor-mediated signaling and competitive binding shed soluble receptor to its ligand. Thus, have both biological importance diagnostic as biomarkers in immunological disorders. Signal regulatory protein α (SIRPα), one responsible for ‘don’t-eat-me’ signal, is expressed by myeloid cells where expression function are part regulated proteolytic cleavage. However, reports on SIRPα a biomarker limited. We...
Hepatic dysfunction is one of the clinical features in severe malaria. However, mechanism hepatic injury during malaria still unknown. Myeloid-related protein (MRP) 14 abundantly expressed by myeloid cells and involved various inflammatory diseases. We previously reported that serum MRP14 elevated mice infected with Plasmodium berghei ANKA. In order to verify whether extracellular pathology rodent malaria, we intravenously administrated recombinant (rMRP14) P. The administration rMRP14 did...
Abstract Background Leishmaniasis is not endemic in Japan, and imported cases are rare. However, there increasing concerns regarding of cutaneous leishmaniasis from countries to Japan. This report describes a case that was diagnosed treated Case presentation A 53-year-old Pakistani man presented with skin lesions on both malleoli his right ankle the dorsum left foot. The manifested as erythematous nodules surrounding an ulcer center lesion. each measured 3 × cm, lesion top foot 5 4 cm. He...
Visceral leishmaniasis (VL) is a major problem worldwide and causes significant morbidity mortality. Existing drugs against VL have limitations, including their invasive means of administration long duration treatment regimens. There are also concerns regarding increasing relapses as well the identification resistant clinical strains with use miltefosine, sole oral drug for VL. is, therefore, an urgent need new alternative In present study, we show leishmanicidal effect novel, antimalarial...
Phlebotomus argentipes is a predominant vector of Leishmania donovani, the protozoan parasite causing visceral leishmaniasis in Indian subcontinent. In hosts bitten by P. argentipes, sand fly saliva elicits production specific anti-salivary protein antibodies. Here, we have utilised these antibodies as markers human exposure to endemic area Pabna district, Bangladesh. The use whole salivary gland homogenate an antigen detect has several limitations, therefore it being superseded recombinant...
The Amami rabbit, Pentalagus furnessi (Mammalia: Lagomorpha: Leporidae), is a relict and endangered species endemic to the Amami-Oshima Tokunoshima Islands, located in southwestern Japan. Here, we described three new of Eimeria (Apicomplexa: Eimeriidae) parasites detected from fecal samples wild rabbits. n. sp., recorded 21 (58.3%) samples, has ellipsoidal oocysts with two walls micropyle, 26.0 × 16.6 μm, elongate-ovoidal sporocysts, 13.1 6.3 Stieda body. hilleri 9 (25.0%) 34.7 21.4...
The prevalence of Toxoplasma gondii infection in free-ranging cats on Tokunoshima Island was assessed by testing 125 serum samples using anti-T. IgG indirect enzyme-linked immunosorbent assay. overall seropositivity rate 47.2% (59/125). Seropositivity rates with body weight >2.0 kg (57.4%) were significantly higher than those ≤2.0 (12.5%, P<0.01). Analysis the number seropositive settlement revealed presence possibly-infected 17 23 settlements, indicating widespread T. island. This is first...