Pancreatic ductal adenocarcinoma (PDAC) is associated with a high incidence of hepatic metastases, as well occasional pulmonary metastases. To delineate the potential role cancer stem cells (CSCs) in PDAC metastasis, human were injected into spleen mice. The characteristics and expression markers CSC epithelial-mesenchymal transition (EMT) metastatic that developed liver lung then compared parental cells. polygonal, larger than Metastatic also exhibited decreased proliferation increased...

Abstract Pancreatic cancer, composed of heterogeneous cancer cells, alters epithelial to mesenchymal features during growth and metastasis. In this study, we aimed characterize pancreatic ductal adenocarcinoma (PDAC) cells showing or in 3D culture. culture, PK-1 had high E-cadherin low vimentin expression exhibited a round-like appearance encircled by flat cells. PANC-1 formed grape-like spheres. secretary granules many microvilli, desmosomes, adherens junctions, while few junction, no...

The Ryukyu long-furred rat, Diplothrix legata (Rodentia: Muridae), is an endangered species endemic to the Tokunoshima, Amami-Oshima, and Okinawajima Islands, which are part of Japan's southwestern islands. During pathological examination a deceased wild individual from Tokunoshima Island, lymphoma infection with three nonnative parasites, Angiostrongylus cantonensis (Nematoda: Angiostrongylidae), Trichosomoides crassicauda Trichosomoididae), Hydatigera taeniaeformis (Cestoda: Taeniidae),...

Elaeophora (Nematoda: Onchocercidae), a filarial nematode infecting the blood vessels of ruminants and horses, is transmitted by tabanid flies. elaphi was previously detected in wild sika deer Wakayama Prefecture, Japan 2009; however, detailed information on this species scarce. In 2023, 26 worms were collected from hepatic vasculature eight Nara, Mie, Kyoto Gifu Prefectures analyzed. Species identification performed morphological genetic analyses. Additionally, multi-gene analysis seven...

The alternative splicing of the extracellular domain fibroblast growth factor receptor (FGFR)-2 generates IIIb and IIIc isoforms. Expression FGFR-2 correlates with vascular endothelial factor-A (VEGF-A) expression venous invasion pancreatic ductal adenocarcinoma (PDAC). By contrast, faster development liver metastasis after surgery, increased proliferation rates cancer. In this study, we analyzed roles total (both isoforms) to determine effectiveness FGFR-2-targeting therapy for PDAC....

Signaling pathways involving signal transducer and activator of transcription 3 (STAT3) play key roles in the aggressiveness pancreatic ductal adenocarcinoma (PDAC), including their tumorigenesis, invasion, metastasis. Cancer stem cells (CSCs) have been correlated with PDAC aggressiveness, activation STAT3 is involved regulation CSC properties. Here, we investigated involvement interleukin-6 (IL-6) or leukemia inhibitory factor (LIF)/glycoprotein 130 (gp130)/STAT3 pathway role CSCs. In...

Fibroblast growth factor receptor 4 (FGFR4), one of four tyrosine kinase receptors for FGFs, is involved in diverse cellular processes. Activation FGF19/FGFR4 signaling closely associated with cancer development and progression. In this study, we examined the expression roles human pancreatic ductal adenocarcinoma (PDAC). PDAC cases, FGFR4 positively correlated larger primary tumors more advanced stages. Among eight cell lines, was expressed at highest levels PK-1 cells, which...

Routinely diagnosed simple solid carcinoma (SSC) of the canine mammary gland comprises a heterogeneous group tumors. Seventy-two cases that had been as SSC based on hematoxylin and eosin–stained tissue sections were reclassified immunohistochemically basis myoepithelial markers p63 α-smooth muscle actin, well luminal epithelial marker cytokeratin 8. Only 23 (32%) true SSC, composed only cells, whereas 11 (15%) malignant myoepithelioma (MM), predominantly 38 (53%) biphasic (BC), characterized...

// Norihiko Sasaki 1 , Masashi Toyoda Hisashi Yoshimura 2 Yoko Matsuda 3 Tomio Arai Kaiyo Takubo 4 Junko Aida and Toshiyuki Ishiwata Research Team for Geriatric Medicine (Vascular Medicine), Tokyo Metropolitan Institute of Gerontology, 173-0015, Japan Division Physiological Pathology, Department Applied Science, School Veterinary Nursing Technology, Nippon Life Science University, 180-8602, Hospital Aging Carcinogenesis, Correspondence to: Ishiwata, email: tishiwat@tmig.or.jp Keywords: H19;...

Nestin, a progenitor/stem cell marker, is expressed in human pancreatic cancer, where its expression correlates positively with invasiveness and metastasis. Here, we investigated the inhibition of nestin regulation expression.We analyzed effects small interfering RNA (siRNA) targeting using cancer lines.Nestin siRNA inhibited growth, migration, invasion, sphere-forming ability lines. Pancreatic cells cotreated gemcitabine exhibited lower viability than treated control siRNA, alone, or alone....

The aims of this study were to determine whether the appearance stromal myofibroblasts and expression tenascin-C (Tn-C) correlate with grade malignancy in canine mammary tumors main cellular source Tn-C these tumors. Single or double immunostaining using antibodies against α-smooth muscle actin (α-SMA) was performed on serial sections normal glands as well those lobular hyperplasia, simple adenoma, carcinoma. Thirty-nine 42 carcinomas (93%) exhibited α-SMA–positive expression. Only 6 11...

Tenascin-C (Tn-C) is an extracellular matrix glycoprotein implicated in the progression of several human cancers. In canine mammary carcinomas, accumulation Tn-C has been recognized 3 different areas: regions proliferating myoepithelial cells complex carcinoma, basement membrane zone low-grade simple and reactive stroma high-grade carcinoma. To identify synthesizing these areas, we utilized double-labeling immunohistochemistry, branched DNA situ hybridization,...

Objective The aim of the study was to investigate clinicopathological features pancreatic cancer at different stages using autopsy results. Methods We retrospectively evaluated 8399 consecutive cases performed between 1972 and 2013 our geriatric hospital. Results Macroscopic lesions were detected in 6.13% cases. Primary secondary tumors observed 2.88% 2.10% cases, respectively. Most primary invasive ductal adenocarcinomas (193 [2.31%]; mean patient age, 78.09 years) with a peak incidence 50...

A 7-year-old female domestic rabbit suffered from labored respiration, poor appetite, mild anemia and thrombocytopenia. Radioscopic examination revealed masses in multiple locations including the intrapleural cavity spleen. Forty-three days after first visit to a private veterinary clinic, died of severe respiratory distress. Microscopically, all were composed round polygonal neoplastic cells with distinct cell borders that arranged sheet pattern. Multinucleated giant often observed. Some...

The Amami spiny rat (Tokudaia osimensis) is an endangered rodent species that endemic to the forests of Amami-Oshima Island, Kagoshima, Japan. In July 2018, a deceased adult male was found on Yuwandake Mountain Trail south-central coast Island. Histopathological observations revealed protozoan infections in liver, lungs, and heart. Nested or semi-nested PCRs targeting B1, SAG3, GRA6, ROP18 genes successfully detected genomic DNA Toxoplasma gondii formalin-fixed paraffin-embedded specimen....

Mammary tumors that spontaneously occurred in domestic Djungarian hamsters (Phodopus sungorus) were histologically examined. Forty-five mammary included 14 adenomas, 18 adenocarcinomas, 1 lipid-rich carcinoma, 2 adenoacanthomas, malignant adenomyoepitheliomas, benign mixed tumor, and 7 "balloon cell" carcinosarcomas. The latter 4 types newly recognized neoplasms hamsters. relatively high incidence of spontaneous carcinosarcomas is intriguing. Carcinosarcomas exhibited anomalous histological...

A 35-mo-old spayed female mixed-breed cat with continuous vomiting, emaciation, and abdominal distention for 2 wk was presented to a private veterinary clinic evaluation. At 71 d after the initial visit, died anemia, jaundice, hypoalbuminemia, subjected autopsy. Grossly, numerous firm masses, 0.5–2.5 cm diameter, were randomly located in left lobe of pancreas. Histologic examination revealed that pancreatic mass consisted tumor cell types: mostly small round cells minority epithelial cells....