A5053052186- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Metastasis and carcinoma case studies
- Gastrointestinal Tumor Research and Treatment
- Helicobacter pylori-related gastroenterology studies
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Esophageal Cancer Research and Treatment
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- Wnt/β-catenin signaling in development and cancer
- Genetic factors in colorectal cancer
- Coenzyme Q10 studies and effects
- Histone Deacetylase Inhibitors Research
- Colorectal Cancer Treatments and Studies
- T-cell and Retrovirus Studies
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Colorectal Cancer Screening and Detection
- HER2/EGFR in Cancer Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pluripotent Stem Cells Research
Maebashi Institute of Technology
2022-2024
Toranomon Hospital
2012-2024
Bayer (Japan)
2016-2024
Tokyo Gas (Japan)
2024
Hamamatsu University School of Medicine
2024
Jefferson Hospital for Neuroscience
2023-2024
University of Miyazaki
2023
Children's National
2020-2023
University of Toyama
2023
Nagoya University Hospital
2022
Introducing a point mutation is fundamental method used to demonstrate the roles of particular nucleotides or amino acids in genetic elements proteins and widely vitro experiments based on cultured cells exogenously provided DNA. However, vivo application this approach by modifying genomic loci uncommon, partly due its technical temporal demands. This leaves many findings un-validated under conditions. We herein applied CRISPR/Cas9 system generate mice with mutations their genomes, which led...
To identify novel methylation‐silenced genes in gastric cancers, we carried out a chemical genomic screening, genome‐wide search for upregulated by treatment with demethylating agent, 5‐aza‐2′‐deoxycytidine (5‐aza‐dC). After 5‐aza‐dC of cancer cell line (AGS) 579 were 16‐fold or more, using an oligonucleotide microarray 39 000 genes. From these genes, selected 44 known on autosomes whose silencing has not been reported. Thirty‐two had CpG islands (CGI) their putative promoter regions, and...
Instructive mechanisms are present for induction of DNA methylation, as shown by methylation specific CpG islands (CGIs) inducers and in cancers. However, instructive factors involved poorly understood, except involvement low transcription trimethylation histone H3 lysine 27 (H3K27me3). Here, we used methylated immunoprecipitation (MeDIP) combined with a CGI oligonucleotide microarray analysis, identified 5510 521 genes promoter CGIs resistant susceptible, respectively, to prostate cancer...
Abstract BACKGROUND: Esophageal squamous cell carcinomas (ESCCs) tend to have multiple primary lesions, and it is believed that they arise from background mucosae with accumulation of genetic/epigenetic alterations. In this study, the objective was elucidate effects smoking drinking on epigenetic alterations in mucosae. METHODS: Genes are silenced human ESCCs were searched for by treating 3 ESCC lines demethylating agent, 5‐aza‐2′‐deoxycytidine performing oligonucleotide microarrays....
The molecular mechanisms underlying the serrated pathway of colorectal tumourigenesis, particularly those related to traditional adenomas (TSAs), are still poorly understood. In this study, we analysed genetic alterations in 188 polyps, including hyperplastic sessile adenomas/polyps (SSA/Ps), TSAs, tubular adenomas, and tubulovillous by using targeted next-generation sequencing reverse transcription-PCR. Our analyses showed that most TSAs (71%) contained WNT components. particular,...
Abstract Background Percutaneous ablation is a common treatment for colorectal liver metastasis (CLM). However, the effect of rat sarcoma viral oncogene homologue (RAS) mutation on outcome after CLMs unclear. Methods Patients who underwent image-guided percutaneous from 2004 to 2015 and had known RAS status were analysed. evaluated local tumour progression as observed imaging treated with ablation. Multivariable Cox regression analysis was performed determine factors associated...
Extensive molecular analyses of ependymal tumors have revealed that supratentorial and posterior fossa ependymomas distinct profiles are likely to be different diseases. The presence C11orf95-RELA fusion genes in a subset (ST-EPN) indicated the existence subgroups. However, pathogenesis RELA fusion-negative remains elusive. To investigate these validate classification tumors, we conducted thorough 113 locally diagnosed from 107 patients Japan Pediatric Molecular Neuro-Oncology Group. All...
Genetic and epigenetic alterations are both involved in carcinogenesis, their low-level accumulation normal tissues constitutes cancer risk. However, relative importance has never been examined, as measurement of mutations difficult. Here, we measured accumulations genetic with low, intermediate, high risk analyzed effects on the esophagus stomach. Accumulation alterations, estimated a frequency rare base substitution mutations, significantly increased according to esophageal mucosae, but...
Objective Cancer-associated fibroblasts (CAFs), a major component of cancer stroma, can confer aggressive properties to cells by secreting multiple factors. Their phenotypes are stably maintained, but the mechanisms not fully understood. We aimed show critical role epigenetic changes in CAFs maintaining their tumour-promoting capacity and validity epigenomic approach identifying therapeutic targets from starve cells. Design Twelve pairs primary gastric corresponding non-CAFs (NCAFs) were...
Aberrant DNA methylation is deeply involved in human cancers, but its inducers and targets are still mostly unclear. Helicobacter pylori infection was recently shown to induce aberrant gastric mucosae, produce a predisposed field for cancerization. Here, we analyzed the presence of target genes induction by H. mechanism gene specificity. Noncancerous mucosae were collected from 4 groups individuals (with without cancer, with current infection; N = 11 each group), promoter CpG islands 48 that...
Residual or locally recurrent lesions may occur after endoscopic therapy for epithelial colorectal tumors. Additional mucosal resection is difficult large lesions. Endoscopic submucosal dissection be useful such lesions, but more technically residual/locally than primary This study evaluated the efficacy of in comparison with lesions.This retrospective case-control investigated 34 and 384 treated using dissection. Tumor size, resected specimen procedure duration, en bloc rate, curative...
Significance To extract critical information from Alzheimer’s disease (AD) postmortem brains that may otherwise be lost, we chose to screen epigenetic signatures. Epigenome analysis is a robust methodology in terms of its cell type and gene specificity, suitability for high-throughput analysis, resistance degradation. Analysis the neuron-specific methylome revealed variety differentially methylated genes, including BRCA1 . We demonstrate pathogenic relevance compromised genomic integrity by...
Down-regulation of the liver-specific MAT1A gene, encoding S-adenosylmethionine (SAM) synthesizing isozymes MATI/III, and up-regulation widely expressed MAT2A , MATII isozyme, known as MAT1A:MAT2A switch, occurs in hepatocellular carcinoma (HCC). Here we found Mat1A:Mat2A switch low SAM levels, associated with CpG hypermethylation histone H4 deacetylation Mat1A promoter, prevalent hypomethylation acetylation Mat2A promoter fast-growing HCC F344 rats, genetically susceptible to...
<b>Background and study aims:</b> The clinical benefit of endoscopic submucosal dissection (ESD) for superficial duodenal neoplasias has not yet been verified. aims this were to validate the feasibility long-term outcomes ESD nonampullary neoplasias, larger than 20 mm. <b>Patients methods:</b> 41 patients, with large who underwent between April 2005 March 2013 included in study. short- retrospectively evaluated, related tumor size, resection histological type, invasion depth, complete rate,...
There is a gradient of β-catenin expression along the colonic crypt axis with highest levels at bottom. In addition, colorectal cancers show heterogeneous subcellular pattern accumulation. However, it remains unclear whether different Wnt signalling exert distinct roles in epithelium. Here, we investigated dose-dependent effect canonical activation on epithelial differentiation by controlling stabilised using doxycycline-inducible transgenic system mice. We that elevated induce amplification...
Alterations of epigenetic modifications are promising targets for cancer therapy, and several drugs now being clinically utilized. At the same time, individual have physiological functions in normal cells, cell specificity is considered difficult to achieve using a drug against single modification. To overcome this limitation, combination specifically or preferentially present cells candidate target. In study, we aimed demonstrate (i) presence cell-specific by focusing on DNA methylation...
Helicobacter pylori (H. pylori) infection induces methylation silencing of tumor suppressor genes causing gastric carcinogenesis. Impairment autophagy DNA damage leading to genetic instability and We aimed identify whether H. induced host autophagy-related (Atg) genes, impairing enhancing Gastric mucosae were obtained from 41 cancer patients 11 healthy volunteers (8 pylori-uninfected 3 pylori-infected). Methylation status Atg was analyzed by a microarray quantitative methylation-specific PCR...