A5022553135- Helicobacter pylori-related gastroenterology studies
- Cancer Cells and Metastasis
- Inflammatory mediators and NSAID effects
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Immune cells in cancer
- TGF-β signaling in diseases
- Gut microbiota and health
- Diet and metabolism studies
- Cancer-related Molecular Pathways
- Wnt/β-catenin signaling in development and cancer
- Inflammation biomarkers and pathways
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Cancer, Stress, Anesthesia, and Immune Response
- Estrogen and related hormone effects
- Pancreatic and Hepatic Oncology Research
- Colorectal Cancer Treatments and Studies
- Gastrointestinal Tumor Research and Treatment
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
Kanazawa University
2016-2025
Life Science Institute
2017-2025
Juntendo University
2024
Nihon University
2023
Yamaguchi University
2023
Japan Agency for Medical Research and Development
2016-2019
In-Q-Tel
2014
Kyoto University
1990-2011
Vehicle Technologies Office
2009
Merck (Japan)
1995-2004
The inflammatory bowel disease ulcerative colitis (UC) frequently progresses to colon cancer. To understand the mechanisms by which UC patients develop carcinomas, we used a mouse model of whereby administration azoxymethane (AOM) followed repeated dextran sulfate sodium (DSS) ingestion causes severe colonic inflammation and subsequent development multiple tumors. We found that treating WT mice with AOM DSS increased TNF-alpha expression number infiltrating leukocytes expressing its major...
Mutations in the APC (adenomatous polyposis coli) gene appear to be responsible for not only familial adenomatous but also many sporadic cases of gastrointestinal cancers. Using homologous recombination mouse embryonic stem cells, we constructed mice that contained a mutant encoding product truncated at 716 (Apc delta 716). Mendelian transmission caused most homozygous die utero before day 8 gestation. The heterozygotes developed multiple polyps throughout intestinal tract, mostly small...
Obesity increases the risk of cancers, including hepatocellular carcinomas (HCC). However, precise molecular mechanisms through which obesity promotes HCC development are still unclear. Recent studies have shown that gut microbiota may influence liver diseases by transferring its metabolites and components. Here, we show hepatic translocation obesity-induced lipoteichoic acid (LTA), a Gram-positive microbial component, creating tumor-promoting microenvironment. LTA enhances...
The cellular interactions in the tumor microenvironment of colorectal cancer (CRC) are poorly understood, hindering patient treatment. In current study, we investigate whether events occurring at invasion front particular importance for CRC treatment strategies. To this end, analyze tissues by combining spatial transcriptomics from patients with a public single-cell transcriptomic atlas to determine cell-cell front. We show that cells localized specifically These induce human leukocyte...
Liver metastases are associated with poor response to current pharmacological treatments, including immunotherapy. We describe a lentiviral vector (LV) platform selectively engineer liver macrophages, Kupffer cells and tumor-associated macrophages (TAMs), deliver type I interferon (IFNα) metastases. Gene-based IFNα delivery delays the growth of colorectal pancreatic ductal adenocarcinoma in mice. Response is TAM immune activation, enhanced MHC-II-restricted antigen presentation reduced...
Mutations in the human adenomatous polyposis (APC) gene are causative for familial (FAP), a rare condition which numerous colonic polyps arise during puberty and, if left untreated, lead to colon cancer. The APC is tumor suppressor that has been termed "gatekeeper gene" In addition 100% mutation rate FAP patients, mutated >80% of sporadic and intestinal cancers. Apc mice either by chemical carcinogenesis, resulting Min mouse Apcdelta850, or heterologous recombination, Apcdelta716...
Selenoprotein biosynthesis is mediated by tRNASec, which inserts selenocysteine at UGA codons in a complex, context-specific manner. This opal suppressor serves the conversion of serine to as well. The mouse tRNASec gene (Trsp) maps proximal segment chromosome 7. We constructed mice carrying targeted deletion Trsp gene. heterozygous mutants were viable, fertile, and appeared normal. Although level was reduced about 50%-80% wild type most organs, one selenoproteins, glutathione peroxidase,...
Rho-associated kinase (ROCK) I mediates signaling from Rho to the actin cytoskeleton. To investigate in vivo functions of ROCK-I, we generated ROCK-I–deficient mice. Loss ROCK-I resulted failure eyelid closure and ventral body wall, which gave rise eyes open at birth omphalocele phenotypes neonates. Most ROCK-I−/− mice died soon after as a result cannibalization by mother. Actin cables that encircle eye epithelial cells were disorganized accumulation filamentous umbilical ring was impaired,...
Abstract Chemokines and their receptors play key roles in leukocyte trafficking are also implicated cancer metastasis to specific organs. Here we show that mouse B16F10 melanoma cells constitutively express chemokine receptor CXCR3, its ligands CXCL9/Mig, CXCL10/IP-10, CXCL11/I-TAC induce cellular responses vitro, such as actin polymerization, migration, invasion, cell survival. To determine whether CXCR3 could a role lymph nodes (LNs), constructed with reduced expression by antisense RNA...
Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein involved in signaling from Rho to actin cytoskeleton.However, vivo functions of each ROCK isoform remain largely unknown.We generated mice deficient by gene targeting.ROCK-II ؊/؊ embryos were found at expected Mendelian frequency until 13.5 days postcoitum, but approximately 90% died thereafter utero.ROCK-II both genders that survived born runts, subsequently developed without gross abnormality,...