A5008236482- Melanoma and MAPK Pathways
- Hippo pathway signaling and YAP/TAZ
- Cancer Cells and Metastasis
- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Pancreatic function and diabetes
- Cancer Research and Treatments
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Virus-based gene therapy research
- Digestive system and related health
- Protein Kinase Regulation and GTPase Signaling
- 3D Printing in Biomedical Research
- Immune Response and Inflammation
- Fungal Biology and Applications
- Electrohydrodynamics and Fluid Dynamics
- Photochromic and Fluorescence Chemistry
- PI3K/AKT/mTOR signaling in cancer
- Neurogenesis and neuroplasticity mechanisms
- Lung Cancer Treatments and Mutations
- Ovarian cancer diagnosis and treatment
- Nerve injury and regeneration
- Complement system in diseases
Hokkaido University
2019-2025
Kyoto University
2006-2019
Japan Science and Technology Agency
2010-2015
Many chemical mediators regulate neutrophil recruitment to inflammatory sites. Although the actions of each mediator have been demonstrated with neutrophils in vitro, how such act cooperatively or counteractively vivo remains largely unknown. Here, by two-photon excitation microscopy transgenic mice expressing biosensors based on Förster resonance energy transfer, we time-lapse–imaged activities extracellular signal–regulated kinase (ERK) and protein A (PKA) inflamed intestinal tissue. ERK...
Acting downstream of many growth factors, extracellular signal-regulated kinase (ERK) plays a pivotal role in regulating cell proliferation and tumorigenesis, where its spatiotemporal dynamics, as well strength, determine cellular responses. Here, we uncover the ERK activity dynamics intestinal epithelial cells (IECs) their association with tumour characteristics. Intravital imaging identifies two distinct modes activity, sustained pulse-like IECs. The activities depend on ErbB2 EGFR,...
Neural regeneration is extremely difficult to achieve. In traumatic brain injuries, the loss of parenchyma volume hinders neural regeneration. this study, neuronal tissue engineering was performed by using electrically charged hydrogels composed cationic and anionic monomers in a 1:1 ratio (C1A1 hydrogel), which served as an effective scaffold for attachment stem cells (NSCs). 3D environment porous C1A1 engineered cryogelation technique, NSCs differentiated into neuroglial cells. The...
Colorectal cancer (CRC) represents a major malignancy that poses significant threat to human health worldwide. The establishment of reliable and pathologically relevant orthotopic model CRC is crucial for gaining deeper understanding its molecular mechanisms developing more effective therapies. Nonetheless, the development such models fraught with challenges primarily owing technical complexities associated transplantation cells into intestinal epithelium. luminal surface cecum was...
Based on a known Wnt agonist BML-284 we designed and synthesized photoswitchable azo derivatives that act as agonists for the signaling pathway. The undergo reversible trans –...
Tribbles encode an evolutionarily conserved protein family that regulates cell proliferation, motility, metabolism and oncogenic transformation. Emerging evidence suggests function as adaptor or scaffold proteins to facilitate the degradation of their target control activation various key signaling pathways. In this study, we uncover a novel human homolog 1 (Trib1) regulator retinoic acid receptor (RAR) signaling. We show shRNA‐mediated knockdown Trib1 promotes transcriptional activity RARs,...
Fat accumulation with aging is a serious problem; glucose-dependent insulinotropic polypeptide/gastric inhibitory polypeptide (GIP) an incretin that plays important role in fat accumulation. GIP receptor knockout mice show reduced mass and improved insulin sensitivity associated aging. Therefore, involved resistance However, age-related changes of secretion remain unclear. The present study aimed to elucidate enteroendocrine K cells using reporter [GIP-green fluorescent protein (GFP)...
Deregulated activation of RAS/extracellular signal-regulated kinase (ERK) signaling and defects in retinoic acid receptor (RAR) are both implicated many types cancers. However, interrelationships between these alterations regulating cancer cell fates have not been fully elucidated. Here, we show that RAS/ERK RAR pathways antagonistically interact with each other to regulate colorectal (CRC) fates. We promotes spontaneous differentiation CRC cells, while ERK suppresses it. Our microarray...
Aging-associated alterations of cellular functions have been implicated in various disorders including cancers. Due to difficulties identifying aging cells living tissues, most studies focused on aging-associated changes whole tissues or certain cell pools. Thus, it remains unclear what kinds accumulate each during aging. While analyzing several mouse lines expressing fluorescent proteins (FPs), we found that expression FPs is gradually silenced the intestinal epithelium units single crypt...
Once inflammation occurs in tissues, response to the gradient of chemoattractants such as leuko triene B 4 (LTB ), IL8, and formylmethionyl leucylphenylalanine (fMLP), neutrophils begin emigrate from inside venules flammatory sites (Phillipson Kubes, 2011).Using various vivo microscopy approaches, it has been demonstrated that neutrophil recruitment involves four steps: rolling, adhesion, crawling, transmigration (Borregaard, 2010;
Extracellular signal-regulated kinase (ERK) plays a critical role in tissue homeostasis and tumorigenesis. By utilizing live imaging approaches, we recently uncovered ERK activity dynamics the intestinal epithelium. Notably, showed that are defined by composite regulation from two distinct upstream receptors, alteration of their functional balance underlies tumor cell-specific traits. Here, discuss these findings.
Abstract Intestinal stem cells produce all types of intestinal epithelial including absorptive enterocytes and secretory to maintain the homeostasis tract. Activity various signaling pathways not only in but also stromal must be coordinately regulated intact structure. Among them, Wnt pathway is regarded as one most critical for cell colon cancer development, which often activated due mutations tumor suppressor gene APC. However, it still unclear how activation causes development. To...