A5017606167- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Colorectal Cancer Treatments and Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Gastric Cancer Management and Outcomes
- Cancer-related Molecular Pathways
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Circular RNAs in diseases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Kruppel-like factors research
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Genetics and Neurodevelopmental Disorders
- DNA Repair Mechanisms
- Folate and B Vitamins Research
- Lung Cancer Treatments and Mutations
- RNA Research and Splicing
Kyushu University Beppu Hospital
2016-2025
Kyushu University
2010-2023
Molecular Oncology (United States)
2008-2023
National Cancer Center Hospital East
2022
The University of Tokyo
2011-2022
Kyushu University Hospital
2012-2022
Japanese Red Cross Medical Center
2022
Tokyo University of Science
2022
The Cancer Institute Hospital
2022
Kagoshima University
2016
The functional impact of recently discovered long noncoding RNAs (ncRNAs) in human cancer remains to be clarified. One ncRNA which has attracted attention is the Hox transcript antisense intergenic RNA termed HOTAIR, a expressed from developmental HOXC locus located on chromosome 12q13.13. In cooperation with Polycomb complex PRC2, HOTAIR reported reprogram chromatin organization and promote breast metastasis. this study, we examined status function patients stage IV colorectal (CRC) who...
Cancer stem cells (CSCs) are generally dormant or slowly cycling tumor that have the ability to reconstitute tumors. They thought be involved in resistance chemo/radiation therapy and relapse progression. However, neither their existence nor identity within many cancers has been well defined. Here, we demonstrated CD13 is a marker for semiquiescent CSCs human liver cancer cell lines clinical samples targeting these might provide way treat this disease. CD13+ predominated G0 phase of cycle...
The functional roles of SNPs within the 8q24 gene desert in cancer phenotype are not yet well understood. Here, we report that CCAT2 , a novel long noncoding RNA transcript (lncRNA) encompassing rs6983267 SNP, is highly overexpressed microsatellite-stable colorectal and promotes tumor growth, metastasis, chromosomal instability. We demonstrate MYC miR–17–5p, miR–20a up-regulated by through TCF7L2-mediated transcriptional regulation. further identify physical interaction between TCF7L2...
Abstract A subset of stem cells, termed “side population” (SP) has been identified and characterized in several mammalian tissues cell lines. However, SP cells have never or isolated from gastrointestinal cancers. We used flow cytometry the DNA-binding dye Hoechst 33342 to isolate various human system cancer Fifteen sixteen lines contained 0.3%–2.2% cells. Next, we an oligonucleotide microarray analyze differentially expressed genes between non-SP hepatoma HuH7. The expression GATA6, which...
Functional microRNAs (miRNAs) in exosomes have been recognised as potential stable biomarkers cancers. The aim of this study is to identify specific miRNAs exosome serum for the early detection recurrence human colorectal cancer (CRC). Serum samples were sequentially obtained from six patients with and without recurrent CRC. purified exosomes, miRNA microarray analysis was performed. expression profiles copy number aberrations explored using array CGH analyses 124 CRC tissues. Then, we...
Esophageal squamous cell carcinoma (ESCC) is the predominant form of esophageal cancer in Japan. Smoking and drinking alcohol are environmental risk factors for ESCC, whereas single nucleotide polymorphisms ADH1B ALDH2, which increase harmful intermediates produced by alcohol, genetic factors. We conducted a large-scale genomic analysis ESCCs from patients Japan to determine mutational landscape this cancer.
Using multiple genetic markers, including cancer stem-like cells, we evaluated the clinical significance of circulating tumor cells (CTCs) as a prognostic factor for overall survival (OS) and disease-free (DFS) in peripheral blood (PB) patients with colorectal (CRC) who had undergone curative surgery.In multi-institutional study, 735 CRC were assigned to retrospective training set (n = 420) or prospective validation 315). CTCs that expressed carcinoembryonic antigen (CEA), cytokeratin (CK)...
Predictive biomarkers for the recurrence of hepatocellular carcinoma (HCC) have great benefit in selection treatment options, including liver transplantation (LT), HCC. The purpose this study was to identify specific microRNAs (miRs) exosomes from serum patients with recurrent HCC and validate these molecules as novel recurrence.We employed microarray-based expression profiling miRs derived a biomarker that distinguishes between without after LT. This followed by validation separate cohort...
Although cancer is a disease with genetic and epigenetic origins, the possible effects of reprogramming by defined factors remain to be fully understood. We studied induction or inhibition cancer-related genes immature status-related whose alterations have been reported in gastrointestinal cells. Retroviral-mediated introduction induced pluripotent stem (iPS) cell was necessary for inducing expression proteins, including Nanog, Ssea4, Tra-1-60, Tra-1-80 esophageal, stomach, colorectal,...
MicroRNA 125a-5p (miR-125a-5p) has been reported to be a tumor suppressor in malignancies of the breast, ovary, lung, and central nervous system. However, clinical significance miR-125a-5p human gastrointestinal cancer not explored. We investigated inhibitory effect gastric cancer, focusing particular on miR-125a-ERBB2 (HER2, HER-2/neu) pathway.Quantitative RT-PCR was used evaluate expression 87 cases determine clinicopathologic expression. The regulation ERBB2 by examined with precursor...
Circulating tumor cells (CTC) in blood have attracted attention both as potential seeds for metastasis and biomarkers. However, most CTC detection systems might miss epithelial-mesenchymal transition (EMT)-induced metastatic because is based on epithelial markers. First, to discover novel markers capable of detecting CTCs which EMT has not been repressed, microarray analysis 132 colorectal cancers (CRC) from Japanese patients was conducted, 2,969 genes were detected that overexpressed...
Pyruvate kinase M2 (PKM2) is an alternatively spliced variant of the pyruvate gene that preferentially expressed during embryonic development and in cancer cells. PKM2 alters final rate-limiting step glycolysis, resulting cancer-specific Warburg effect (also referred to as aerobic glycolysis). Although previous reports suggest functions nonmetabolic transcriptional regulation, its significance biology remains elusive. Here we report stimulation epithelial-mesenchymal transition (EMT) results...
Abstract The biological significance of micro (mi)RNAs has traditionally been evaluated according to their RNA expression levels based on the assumption that miRNAs recognize and regulate targets in an unvarying fashion. Here we show a fraction mature including miR-17-5p, -21-5p, -200c-3p let-7a-5p harbor methyl marks potentially alter stability target recognition. Importantly, methylation these was significantly increased cancer tissues as compared paired normal tissues. Furthermore,...
The cellular interactions in the tumor microenvironment of colorectal cancer (CRC) are poorly understood, hindering patient treatment. In current study, we investigate whether events occurring at invasion front particular importance for CRC treatment strategies. To this end, analyze tissues by combining spatial transcriptomics from patients with a public single-cell transcriptomic atlas to determine cell-cell front. We show that cells localized specifically These induce human leukocyte...
Enhancer of zeste homologue 2 (EZH2), a member the polycomb group protein family, plays crucial role in regulation embryonic development and has been associated with cell cycle. Recently, several studies have shown that EZH2 is highly expressed aggressive tumours, including human breast cancer, prostate lymphomas. We thus analysed expression using real-time reverse transcription–polymerase chain reaction, correlated its status various clinicopathological parameters 66 patients hepatocellular...
Detection of the mRNA selected genes by reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive and powerful tool for detecting cancer cells in bone-marrow or peripheral-blood samples. In this study, we determined whether carcinoembryonic antigen (CEA) detectable peripheral blood patients with gastrointestinal breast cancer. addition, studied undergoing surgical procedures to assess tumor manipulation during operation enhances cancer-cell dissemination. Peripheral from 55 22...
Abstract Purpose and Experimental Design: To identify cancer-related genes, the expression profiles of colorectal cancer cells normal epithelial were examined compared using laser microdissection cDNA microarray analysis. From these combined techniques, several including TROP2, identified. TROP2 is known as a calcium signal transducer highly expressed in types tumors. However, no studies have investigated significance cancer. Thus, status was 74 samples by quantitative real-time reverse...