A5101822595- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Cancer Research and Treatments
- Prostate Cancer Treatment and Research
- RNA Interference and Gene Delivery
- Toxin Mechanisms and Immunotoxins
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- Hepatitis B Virus Studies
- Reproductive System and Pregnancy
- Research on Leishmaniasis Studies
- Cytokine Signaling Pathways and Interactions
- HIV Research and Treatment
- Immune Response and Inflammation
- Trypanosoma species research and implications
- Chemokine receptors and signaling
- HER2/EGFR in Cancer Research
National Institutes of Health
2015-2024
Center for Cancer Research
2013-2023
National Cancer Institute
2014-2023
Cancer Institute (WIA)
2023
Occupational Cancer Research Centre
2020
ORCID
2007-2015
Institut de Recherche Vaccinale
2005-2014
National Cancer Institute
2007-2013
University of Birmingham
2013
National Institute of Allergy and Infectious Diseases
2011
Primary liver tumors and metastasis currently represent the leading cause of cancer-related death. Commensal bacteria are important regulators antitumor immunity, although is exposed to gut bacteria, their role in surveillance poorly understood. We found that altering commensal mice induced a liver-selective effect, with an increase hepatic CXCR6+ natural killer T (NKT) cells heightened interferon-γ production upon antigen stimulation. In vivo functional studies showed NKT mediated tumor...
Our previous work demonstrated that cytotoxic T lymphocyte (CTL)-mediated tumor immunosurveillance of the 15-12RM could be suppressed by a CD1d-restricted lymphocyte, most likely natural killer (NK) cell, which produces interleukin (IL)-13. Here we present evidence for effector elements in this suppressive pathway. cell–reconstituted recombination activating gene (RAG)2 knockout (KO) and RAG2/IL-4 receptor α double KO mice showed inhibition requires IL-13 responsiveness non–T non–B cell....
Abstract Extracellular vesicles (EVs), including exosomes and microvesicles, are 30–800 nm that released by most cell types, as biological packages for intercellular communication. Their importance in cancer inflammation makes EVs their cargo promising biomarkers of disease cell-free therapeutic agents. Emerging high-resolution cytometric methods have created a pressing need efficient fluorescent labeling procedures to visualize detect EVs. Suitable labels must be bright enough one EV...
The importance of immunoregulatory T cells has become increasingly apparent. Both CD4+CD25+ and CD1d-restricted NKT have been reported to down-regulate tumor immunity in mouse models. However, the relative roles both cell populations rarely clearly distinguished same In addition, play a critical role not only down-regulation but also promotion immunity. explanation for these apparently opposite different models remains unclear. We show that four which suppression immunity, depletion did...
Abstract Overexpression of the immunosuppressive cytokine transforming growth factor β (TGF-β) is one strategy that tumors have developed to evade effective immunesurveillance. Using transplantable models breast and colon cancer, we made unexpected finding CD8+ cells in tumor-bearing animals can directly promote tumorigenesis, by a mechanism dependent on TGF-β. We showed splenocytes from mice expressed elevated interleukin (IL)-17 when compared with naive mice, T could be induced make IL-17...
Overexpression of transforming growth factor beta (TGF-beta) is frequently associated with metastasis and poor prognosis, TGF-beta antagonism has been shown to prevent in preclinical models surprisingly little toxicity. Here, we have used the transplantable 4T1 model metastatic breast cancer address underlying mechanisms. We showed that efficacy anti-TGF-beta antibody 1D11 suppressing was dependent on a synergistic combination effects both tumor parenchyma microenvironment. The main outcome...
Abstract Negative immunoregulation is a major barrier to successful cancer immunotherapy. The NKT cell known be one such regulator. In this study we explored the roles of and interaction between classical type I poorly understood II in regulation tumor immunity. Selective stimulation cells suppressed immunosurveillance, whereas protected against growth even when responses were relatively skewed toward Th2 cytokines. When both stimulated simultaneously, appeared suppress activation vitro...
Abstract Although significant advances in radiotherapy have increased its effectiveness many cancer settings, general strategies to widen the therapeutic window between normal tissue toxicity and malignant tumor destruction would still offer great value. CD47 blockade has been found confer radioprotection tissues while enhancing radiosensitivity. Here, we report that directly enhances immunosurveillance by CD8+ T cells. Combining with irradiation did not affect fibrosarcoma growth...
CD4+ helper T cells contribute to the induction and maintenance of antigen-specific CD8+ cells. Their absence results in short-lived defective secondary cell responses because tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. Here, we show that IL-15 codelivered with vaccines can overcome deficiency for promoting longevity avoidance TRAIL-mediated In both priming responses, down-regulates proapoptotic Bax, an intermediate apoptosis, increases anti-apoptotic...
ABSTRACT Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields medicine as biomarkers mediators or treatments for disease. However, exosomes small fall below the detection limits conventional flow cytometers due overlap particle‐associated scattered light signals with background instrument noise from diffusely light. To identify, sort, study distinct subsets EVs other individual particles, we developed nanoscale Fluorescence Analysis...
Abstract Purpose: Transforming growth factor-β (TGF-β) is an immunosuppressive cytokine, having direct suppressive activity against conventional CD4+ and CD8+T cells natural killer cells, thereby inhibiting tumor immunosurveillance. Here, we investigated possible synergy between anti–TGF-β (1D11) a peptide vaccine on induction of antitumor immunity, the mechanisms accounting for synergistic efficacy. Experimental Design: The effect combination treatment with was examined in subcutaneous TC1...