A5088145657- Spider Taxonomy and Behavior Studies
- Pluripotent Stem Cells Research
- Venomous Animal Envenomation and Studies
- Renal and related cancers
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Beetle Biology and Toxicology Studies
- Identification and Quantification in Food
- Neurobiology and Insect Physiology Research
- Single-cell and spatial transcriptomics
- Insect symbiosis and bacterial influences
- Chromosomal and Genetic Variations
- Antimicrobial Peptides and Activities
- Environmental Science and Water Management
- Epigenetics and DNA Methylation
- Neurogenesis and neuroplasticity mechanisms
- Pleistocene-Era Hominins and Archaeology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Retinal Development and Disorders
- Forensic Anthropology and Bioarchaeology Studies
- Archaeology and ancient environmental studies
- Mosquito-borne diseases and control
- Zebrafish Biomedical Research Applications
University of California, San Francisco
2020-2024
Gladstone Institutes
2020-2024
University of California, San Diego
2017-2020
Abstract Cas9/gRNA-mediated gene-drive systems have advanced development of genetic technologies for controlling vector-borne pathogen transmission. These include population suppression approaches, analogs insecticidal techniques that reduce the number insect vectors, and modification (replacement/alteration) which interfere with competence to transmit pathogens. Here, we develop a recoded rescue system malaria vector, Anopheles stephensi , relieves load in females caused by integration...
Axial elongation of the neural tube is crucial during mammalian embryogenesis for anterior-posterior body axis establishment and subsequent spinal cord development, but these processes cannot be interrogated directly in humans as they occur post-implantation. Here, we report an organoid model extension derived from human pluripotent stem cell (hPSC) aggregates that have been caudalized with Wnt agonism, enabling them to recapitulate aspects morphological temporal gene expression patterns...
In the nascent mesoderm, TBXT expression must be precisely regulated to ensure that cells exit primitive streak and pattern anterior-posterior axis, but how varying dosage informs morphogenesis is not well understood. this study, we define transcriptional consequences of reduction during early human gastrulation using induced pluripotent stem cell models mesoderm differentiation. Multi-omic single-nucleus RNA ATAC sequencing 2D gastruloids comprising wild-type, heterozygous or null reveal...
ABSTRACT Loss of Cdx2 in vivo leads to stunted development the allantois, an extraembryonic mesoderm-derived structure critical for nutrient delivery and waste removal early embryo. Here, we investigate how CDX2 dose-dependently influences gene regulatory network underlying mesoderm development. By engineering human induced pluripotent stem cells (hiPSCs) consisting wild-type (WT), heterozygous (CDX2-Het), homozygous null (CDX2-KO) genotypes, differentiating these a 2D gastruloid model,...
Venom has been associated with the ecological success of many groups organisms, most notably reptiles, gastropods, and arachnids. In some cases, diversification directly linked to tailoring venoms for dietary specialization. Spiders in particular are known their diverse wide range predatory behaviors, although there is much learn about scales variation venom composition function. The current study focuses on characteristics different sexes within a species spider. We chose genus Tetragnatha...
ABSTRACT The development of Cas9/gRNA-mediated gene-drive systems has bolstered the advancement genetic technologies for controlling vector-borne pathogen transmission. These include population suppression approaches, analogs insecticidal techniques that reduce number vector insects, and modification (replacement/alteration) which interfere with competence to transmit pathogens. We developed a recoded rescue system in malaria vector, Anopheles stephensi , relieves load females caused by...
In the nascent mesoderm, levels of Brachyury (TBXT) expression must be precisely regulated to ensure cells exit primitive streak and pattern anterior-posterior axis, but how this varying dosage informs morphogenesis is not well understood. study, we define transcriptional consequences TBXT dose reduction during early human gastrulation using induced pluripotent stem cell (hiPSC)-based models mesoderm differentiation. Multiomic single-nucleus RNA ATAC sequencing 2D gastruloids comprised WT,...
ABSTRACT First Person is a series of interviews with the first authors selection papers published in Biology Open, helping researchers promote themselves alongside their papers. Emily Bulger author on ‘ CDX2 dose-dependently influences gene regulatory network underlying human extraembryonic mesoderm development’, BiO. conducted research described this article while PhD student Benoit Bruneau's lab at Gladstone Institutes, San Francisco, USA. She now postdoctoral scholar investigating how...
During development, early regionalization segregates lineages and directs diverse cell fates. Sometimes, however, distinct progenitors produce analogous types. For example, V2a neurons, are excitatory interneurons that emerge from different anteroposterior progenitors. neurons demonstrate remarkable plasticity after spinal cord injury improve motor function, showing potential for therapy. To examine how lineage origins shape their properties, we differentiated hPSC-derived with identities....
ABSTRACT Proper regulation of gene dosage is critical for the development early embryo and extraembryonic tissues that support it. Specifically, loss Cdx2 in vivo leads to stunted allantois, an mesoderm-derived structure nutrient delivery waste removal embryo. In this study, we investigate how CDX2 dose-dependently influences regulatory network underlying mesoderm development. We generate allelic series human induced pluripotent stem cells (hiPSCs) consisting WT, heterozygous, homozygous...
genes determine positional codes along the head-to-tail axis. Here, we replaced entire
Venom has been associated with the ecological success of many groups organisms, most notably reptiles, gastropods, and arachnids. In some cases, diversification directly linked to tailoring venoms for dietary specialization. Spiders in particular are known their diverse wide range predatory behaviors, although there is much learn about scales variation venom composition function. The current study focuses on characteristics different sexes within a species spider. We chose genus Tetragnatha...
Venom has been associated with the ecological success of many groups organisms, most notably reptiles, gastropods, and arachnids. In some cases, diversification directly linked to tailoring venoms for dietary specialization. Spiders in particular are known their diverse wide range predatory behaviors, although there is much learn about scales variation venom composition function. The current study focuses on characteristics different sexes within a species spider. We chose genus Tetragnatha...