A5001067668- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- RNA modifications and cancer
- Insect Resistance and Genetics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Developmental Biology and Gene Regulation
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Viral Infections and Outbreaks Research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
University of California, San Diego
2020-2024
Centre National de la Recherche Scientifique
2017
Aix-Marseille Université
2017
Centre for DNA Fingerprinting and Diagnostics
2015
Abstract A recurring target-site mutation identified in various pests and disease vectors alters the voltage gated sodium channel ( vgsc ) gene (often referred to as knockdown resistance or kdr confer commonly used insecticides, pyrethroids DDT. The ubiquity of mutations poses a major global threat continued use insecticides means for vector control. In this study, we generate common isogenic laboratory Drosophila strains using CRISPR/Cas9 editing. We identify differential sensitivities...
Repair of double-strand breaks (DSBs) in somatic cells is primarily accomplished by error-prone nonhomologous end joining and less frequently precise homology-directed repair preferentially using the sister chromatid as a template. Here, Drosophila system performs efficient both DSBs single-strand (SSBs) intact sequences from homologous chromosome process we refer to chromosome-templated (HTR). Unexpectedly, HTR-mediated allelic conversion at white locus was more (40 65%) response SSBs...
Development of effective therapies against SARS-CoV-2 infections relies on mechanistic knowledge virus-host interface. Abundant physical interactions between viral and host proteins have been identified, but few functionally characterized. Harnessing the power fly genetics, we develop a comprehensive Drosophila COVID-19 resource (DCR) consisting publicly available strains for conditional tissue-specific expression all encoded proteins, UAS-human cDNA transgenic lines encoding established...
Abstract CRISPR-interference (CRISPRi), a highly effective method for silencing genes in mammalian cells, employs an enzymatically dead form of Cas9 (dCas9) complexed with one or more guide RNAs (gRNAs) 20 nucleotides (nt) complementarity to transcription initiation sites target genes. Such gRNA/dCas9 complexes bind DNA, impeding the targeted locus. Here, we present alternative gene-suppression strategy using active truncated gRNAs (tgRNAs). Cas9/tgRNA specific without triggering DNA...
CRISPR-based active genetic elements, or gene-drives, copied via homology-directed repair (HDR) in the germline, are transmitted to progeny at super-Mendelian frequencies. Active elements also can generate widespread somatic mutations, but basis for such phenotypes remains uncertain. It is generally assumed that mutations generated by non-homologous end-joining (NHEJ), predominant double stranded break pathway cells. Here, we develop CopyCatcher systems Drosophila detect and quantify gene...
The expression and regulation of Hox genes in developing central nervous system (CNS) lack important details like specific cell types where are expressed the transcriptional regulatory players involved these cells. In this study we have investigated Drosophila gene Deformed (Dfd) embryonic CNS. Using Dfd neural autoregulatory enhancer find that autoregulates itself cells mandibular neuromere. We also role a cofactor Homothorax (Hth) for its regulating Hth exhibits region controlling Dfd, but...
Abstract Insecticide resistance (IR) poses a significant global challenge to public health and welfare. Here, we develop locally-acting unitary self-eliminating allelic-drive system, inserted into the Drosophila melanogaster yellow ( y ) locus. The drive cassette encodes both Cas9 single gRNA bias inheritance of favored wild-type (1014 L) allele over IR F) variant voltage-gated sodium ion channel vgsc target When enduring fitness cost, this transiently-acting can increase frequency 100%,...
genes determine positional codes along the head-to-tail axis. Here, we replaced entire