A5053823327- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- Hippo pathway signaling and YAP/TAZ
- Microtubule and mitosis dynamics
- Muscle Physiology and Disorders
- Plant Molecular Biology Research
- Axon Guidance and Neuronal Signaling
- Chromosomal and Genetic Variations
- interferon and immune responses
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Medical and Biological Sciences
- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Protist diversity and phylogeny
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
Institut de Biologie du Développement Marseille
2012-2025
Centre National de la Recherche Scientifique
2014-2025
Aix-Marseille Université
2013-2025
Canadian Nautical Research Society
2013
Centre d’Immunologie de Marseille-Luminy
1995-2011
Université Paris Cité
2010
Epigénétique et Destin Cellulaire
2010
Inserm
2000-2006
University of Rochester
2006
Seattle University
2005
Hox transcription factors are essential for shaping body morphology in development and evolution. The control of protein activity part arises from interaction with the PBC class partners, pre-B cell factor (Pbx) proteins vertebrates Extradenticle (Exd) Drosophila. Characterized interactions occur through a single mode, involving short hexapeptide motif protein. This apparent uniqueness Hox-PBC provides little mechanistic insight how same cofactors endow specific diverse activities. Here, we...
Abstract Background Protein interactions control the regulatory networks underlying developmental processes. The understanding of complexity will, therefore, require characterization protein within their proper environment. bimolecular fluorescence complementation (BiFC) technology offers this possibility as it enables direct visualization in living cells. However, its potential has rarely been applied embryos animal model organisms and was only performed under transient expression levels....
Hox transcription factors control a number of developmental processes with the help PBC class proteins. In vitro analyses have established that formation Hox/PBC complexes relies on short conserved protein motif called hexapeptide (HX). This paradigm is at basis vast majority experimental approaches dedicated to study function. Here we questioned unique and general use HX for recruitment by using Bimolecular Fluorescence Complementation (BiFC) assay. method allows analyzing Hox-PBC...
Hox proteins, a sub-group of the homeodomain (HD) transcription factor family, provide positional information for axial patterning in development and evolution. protein functional specificity is reached, at least part, through interactions with Pbc (Extradenticle (Exd) Drosophila ) Meis/Prep (Homothorax (Hth) proteins. Most our current knowledge stems from study anterior central identifying molecular structural bases Hox/Pbc/Meis-Prep cooperative action. Posterior class Abdominal-B (Abd-B)...
Gene regulation by AP-1 transcription factors in response to Jun N-terminal kinase (JNK) signaling controls essential cellular processes during development and pathological situations. Here, we report genetic molecular evidence that the histone acetyltransferase (HAT) Chameau deacetylase DRpd3 act as antagonistic cofactors of DJun DFos modulate JNK-dependent thorax metamorphosis JNK-induced apoptosis Drosophila . We demonstrate cultured cells phosphorylation mediated JNK plays a central role...
Hox genes in species across the metazoa encode transcription factors (TFs) containing highly-conserved homeodomains that bind target DNA sequences to regulate batteries of developmental genes. DNA-bound proteins, together with other TF partners, induce an appropriate transcriptional response by RNA Polymerase II (PolII) and its associated general factors. How evolutionarily conserved TFs interface this machinery generate finely regulated responses remains obscure. One major component PolII...
ABSTRACT Wnt genes encode putative cell signalling proteins which play crucial roles during development. From a library of DNA fragments associated, in vivo, with Ultrabithorax proteins, we isolated novel Drosophila gene, DWnt-4. Neither paralog nor an ortholog the gene exist current repertoire full-length sequences. DWnt-4 maps close (30 kb) to wingless, suggesting that two derive from duplication occurred early evolution, since they are significantly diverged sequence and structure....
Hox genes encode transcription factors widely used for diversifying animal body plans in development and evolution. To achieve functional specificity, proteins associate with PBC class proteins, Pre-B cell leukemia homeobox (Pbx) vertebrates, Extradenticle (Exd) Drosophila , were thought to use a unique hexapeptide-dependent generic mode of interaction. Recent findings, however, revealed the existence an alternative, UbdA-dependent paralog-specific interaction providing diversity Hox–PBC...
Protein function is encoded within protein sequence and domains. However, how domains cooperate a to modulate overall activity this impacts functional diversification at the molecular organism levels remains largely unaddressed. Focusing on three of central class Drosophila Hox transcription factor AbdominalA (AbdA), we used combinatorial domain mutations most known AbdA developmental functions as biological readouts investigate collectively shape activity. The results uncover redundancy,...
Extradenticle (Exd) and Homothorax (Hth) function as positive transcriptional cofactors of Hox proteins, helping them to bind specifically their direct targets. The posterior protein Abdominal-B (Abd-B) does not require Exd/Hth DNA; and, during embryogenesis, Abd-B represses hth exd transcription. Here we show that this repression is necessary for function, maintained expression results in transformations similar those observed loss-of-function mutants. We characterize the cis regulatory...
Abstract Oxidative metabolism is the predominant energy source for aerobic muscle contraction in adult animals. How cellular and molecular components that support physiology are put place during development through their transcriptional regulation not well understood. Using Drosophila flight model, we show formation of mitochondria cristae harbouring respiratory chain concomitant with a large-scale upregulation genes linked oxidative phosphorylation (OXPHOS) specific stages development. We...
Hox proteins play fundamental roles in generating pattern diversity during development and evolution, acting broad domains but controlling localized cell diversification pattern. Much remains to be learned about how selector generate cell-type diversity. In this study, regulatory specificity was investigated by dissecting the genetic molecular requirements that allow protein Abdominal A activate wingless only a few cells of its expression domain Drosophila visceral mesoderm. We show...