A5056605822- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Developmental Biology and Gene Regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect Resistance and Genetics
- Genetics, Aging, and Longevity in Model Organisms
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Neuropeptides and Animal Physiology
- Diabetes and associated disorders
- Signaling Pathways in Disease
- Diabetes Management and Research
- RNA Research and Splicing
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Vestibular and auditory disorders
- Diabetes Treatment and Management
- Molecular Biology Techniques and Applications
Centre for DNA Fingerprinting and Diagnostics
2015-2025
Columbia University Irving Medical Center
2006-2010
Columbia University
2007-2010
Tata Institute of Fundamental Research
2001-2006
National Centre for Biological Sciences
2001-2006
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2006
Harvard University
2006
Hox proteins frequently select and regulate their specific target genes with the help of cofactors like Extradenticle (Exd) Homothorax (Hth). For Drosophila protein Sex combs reduced (Scr), Exd has been shown to position a normally unstructured portion Scr so that two basic amino acid side chains can insert into minor groove an Scr-specific DNA-binding site. Here we provide evidence another protein, Deformed (Dfd), uses very similar mechanism achieve specificity in vivo, thus generalizing...
Signaling by the second messenger inositol 1,4,5-trisphosphate is thought to affect several developmental and physiological processes. Mutants in receptor (itpr) gene of Drosophila exhibit delays molting while stronger alleles are also larval lethal. In a freshly generated set EMS for itpr locus we have sequenced identified single point mutations seven mutant chromosomes. The predicted allelic strength these mutants matches observed levels lethality. They range from weak hypomorphs complete...
Larval molting in Drosophila, as other insects, is initiated by the coordinated release of steroid hormone ecdysone, response to neural signals, at precise stages during development. In this study we have analyzed, using genetic and molecular methods, roles played two major signaling pathways regulation larval Drosophila. Previous studies shown that mutants for inositol 1,4,5-trisphosphate receptor gene (itpr) are lethals. addition they exhibit delays can be rescued exogenous feeding...
Ionic Ca 2+ functions as a second messenger to control several intracellular processes. It also influences intercellular communication. The release of from stores through the inositol 1,4,5-trisphosphate receptor (InsP 3 R) occurs in both excitable and nonexcitable cells. In Drosophila , InsP R activity is required aminergic interneurons during pupal development for normal flight behavior. By altering levels genetic means, we now show that signaling at multiple steps generating neural...
Hox mediated neuroblast apoptosis is a prevalent way to pattern larval central nervous system (CNS) by different genes, but the mechanism of this not understood. Our studies with Abdominal-A (Abd-A) (pNB) suggests that AbdA, its cofactor Extradenticle (Exd), helix-loop-helix transcription factor Grainyhead (Grh), and Notch signaling transcriptionally contribute expression RHG family apoptotic genes. We find Grh, Exd function together at multiple motifs on enhancer. In vivo mutagenesis these...
Highly conserved DM domain containing transcription factors (Doublesex/MAB-3/DMRT1) are responsible for generating sexually dimorphic features. In Drosophila CNS a set of Doublesex (Dsx) expressing neuroblasts undergo apoptosis in females while their male counterparts proliferate and give rise to serotonergic neurons crucial adult mating behaviour. Our study demonstrates that female specific isoform collaborates with Hox gene Abdominal-B (AbdB) bring about this apoptosis. Biochemical results...
Neural circuitry for mating and reproduction resides within the terminal segments of central nervous system (CNS) which express Hox paralogous group 9–13 (in vertebrates) or Abdominal-B (Abd-B) in Drosophila. Terminal neuroblasts (NBs) A8-A10 Drosophila larval CNS are subdivided into two groups based on expression transcription factor Doublesex (Dsx). While sex specific fate Dsx-positive NBs is well investigated, Dsx-negative not known so far. Our studies with suggests that these cells, like...
The expression and regulation of Hox genes in developing central nervous system (CNS) lack important details like specific cell types where are expressed the transcriptional regulatory players involved these cells. In this study we have investigated Drosophila gene Deformed (Dfd) embryonic CNS. Using Dfd neural autoregulatory enhancer find that autoregulates itself cells mandibular neuromere. We also role a cofactor Homothorax (Hth) for its regulating Hth exhibits region controlling Dfd, but...
Type 2 Diabetes Mellitus (T2DM) is a prevalent metabolic disorder characterized by elevated blood glucose levels. Ayurveda identifies similar condition known as Madhumeha, type of Prameha associated with an imbalance in Kapha and Vata Doshas. This study explores the efficacy Ayurvedic treatment managing T2DM. Methods: A 50-year-old male patient T2DM no significant history major illness was treated using holistic approach. The regimen included formulations such Madhumehari Churna, Ojaswani...
Abstract Hox genes code for a family of homeodomain containing transcription factors that use TALE-HD Pbx/Exd and Meis/Homothorax to specify the development anterior–posterior axis an organism. However, absence from specific tissues emphasizes need identify validate new cofactors. In Drosophila central nervous system, executes segment-specific apoptosis neural stem cells (neuroblasts) neurons. abdominal segments larval gene Abdominal-A mediates neuroblast with help Extradenticle bHLH factor...