A5005274253- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Insect and Pesticide Research
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Insect Utilization and Effects
- Calpain Protease Function and Regulation
- Trypanosoma species research and implications
- Neurobiology and Insect Physiology Research
- Study of Mite Species
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- Insect and Arachnid Ecology and Behavior
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- Physiological and biochemical adaptations
- Connexins and lens biology
- Insect-Plant Interactions and Control
- Plant and animal studies
- Invertebrate Immune Response Mechanisms
- Venomous Animal Envenomation and Studies
- Chromosomal and Genetic Variations
Universidade Federal do Rio de Janeiro
2018-2024
Universidade Estadual de Maringá
2016-2019
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2018
Abstract A recurring target-site mutation identified in various pests and disease vectors alters the voltage gated sodium channel ( vgsc ) gene (often referred to as knockdown resistance or kdr confer commonly used insecticides, pyrethroids DDT. The ubiquity of mutations poses a major global threat continued use insecticides means for vector control. In this study, we generate common isogenic laboratory Drosophila strains using CRISPR/Cas9 editing. We identify differential sensitivities...
is currently the model vector of choice for studying Chagas disease transmission, a debilitating caused by
The piRNA pathway is a surveillance system that guarantees oogenesis and adult fertility in range of animal species. centered on PIWI clade Argonaute proteins the associated small non-coding RNAs termed piRNAs. In this study, we set to investigate evolutionary conservation hemimetabolous insect Rhodnius prolixus. Our transcriptome profiling reveals core components are expressed during previtellogenic stages oogenesis. Rhodnius' genome harbors four putative piwi orthologs. We show Rp-piwi2,...
Abstract The neonicotinoid thiamethoxam is widely used in different agricultural crops, and it has a spectrum of action against insects, affecting both pests pollinators, such as bees. In this study, the effects exposure to sublethal concentrations on stingless bees Scaptotrigona bipunctata were evaluated. Foragers exposed insecticide subjected genetic biochemical, histochemical, morphological analyses after 24, 48, 72 h ingestion. Analysis isoenzyme esterases revealed significant...
Repair of double-strand breaks (DSBs) in somatic cells is primarily accomplished by error-prone nonhomologous end joining and less frequently precise homology-directed repair preferentially using the sister chromatid as a template. Here, Drosophila system performs efficient both DSBs single-strand (SSBs) intact sequences from homologous chromosome process we refer to chromosome-templated (HTR). Unexpectedly, HTR-mediated allelic conversion at white locus was more (40 65%) response SSBs...
Pigmentation in insects has been linked to mate selection and predator evasion, thus representing an important aspect for natural selection. Insect body color is classically associated the activity of tyrosine pathway enzymes, eye pigment synthesis through tryptophan guanine pathways, their transport by ATP-binding cassette proteins. Among hemiptera, genetic basis pigmentation kissing bugs such as Rhodnius prolixus, that transmit Chagas disease humans, not addressed. Here, we report...
Abstract Tribolium castaneum Herbst 1797 (Coleoptera: Tenebrionidae), an important pest of stored grains and byproducts, is naturally infected by Gregarina cuneata Stein 1848 (Apicomplexa: Gregarinidae). Changes in the life cycle insects caused parasite development midgut were studied. Trophozoites, gamonts (solitary associated), gametocysts present insects. In young trophozoites, apical region differentiated into epimerite that firmly attached to host epithelial cells. With maturation,...
Abstract Rhodnius prolixus is currently the model vector of choice for studying Chagas disease transmission, a debilitating caused by Trypanosoma cruzi parasites. Despite broad use double-stranded RNA interference knockdown gene function in R. , transgenesis and editing protocols are still lacking. Here we tested Receptor-Mediated Ovary Transduction Cargo (ReMOT Control) direct parental injection CRISPR (DIPA-CRISPR) maternal delivery CRISPR/Cas9 elements to developing oocytes, strategies...
The calcium dependent Calpain proteases are modulatory enzymes with important roles in cell cycle control, development and immunity. In the fly model Drosophila melanogaster A cleaves Cactus/IkappaB consequently modifies Toll signals during embryonic dorsal-ventral (DV) patterning. Here we explore role of Calpains hemiptera Rhodnius prolixus, an intermediate germband insect where Bone Morphogenetic Protein (BMP) instead pathway plays a major DV Phylogenetic analysis species ranging from...
The calcium dependent Calpain proteases are modulatory enzymes with important roles in cell cycle control, development and immunity. In the fly model
Cotesia flavipes parasitizes Diatraea saccharalis, a pest that causes major losses to sugarcane production. The mass production of C. and its subsequent release onto is one form biological control D. saccharalis. An essential factor for successfully fighting this the development host diets can increase efficiency Palm oil contains saturated fatty acids, unsaturated polyunsaturated linoleic acid. However, little known about effects palm on in laboratory. Different concentrations were added...
O manejo das principais pragas de grãos armazenados tem sido feito, preferencialmente, com o uso inseticida que representa um enorme risco no desenvolvimento resistência. Há muitos estudos Sitophilus zeamais visando seu controle, porém poucos analisando a variabilidade genética desta espécie. Este trabalho teve como objetivo estimar S. em 10 amostras diferentes localidades, destas, oito são do Brasil: Tunápolis (SC), Iguatemi (PR), Piracicaba (SP), Coimbra (MG), Maracaju (MS), Canarana (MT),...