Abstract Bees are essential pollinators that contribute to maintaining biodiversity and increasing agricultural production. However, by foraging on crops, bees may become contaminated with compounds used for pest control. In this study, we exposed bee ( Apis mellifera L.) colonies the insecticide imidacloprid (IMD) under field conditions assess occurrence of oxidative stress in larvae pupae investigate morphological changes fat body midgut adult bees. The apiary area was divided into three...

This experiment was carried out to evaluate the effect of honeybee pollination in production and quality soybean seeds (Glycine max L. Merril). Seed higher (P=0.0001) covered areas with colonies (50.64%) uncovered (57.73%) than without colonies. It could be concluded that honeybees were responsible for 95.5% accomplished by insects. The pod number treatment 61.38% (P=0.0002) honeybees. average weight 100 larger area honeybees, reached 17.8 g. medium content crude protein grains 36.7% oil...

Abstract The neonicotinoid thiamethoxam is widely used in different agricultural crops, and it has a spectrum of action against insects, affecting both pests pollinators, such as bees. In this study, the effects exposure to sublethal concentrations on stingless bees Scaptotrigona bipunctata were evaluated. Foragers exposed insecticide subjected genetic biochemical, histochemical, morphological analyses after 24, 48, 72 h ingestion. Analysis isoenzyme esterases revealed significant...

Bee colony health is declining as a result of several factors, including exposure to pesticides. The development and strength honey bee colonies depend on the reproductive success queen bees. Because flowers are sources food for bees, foragers can accidentally collect carry contaminated pollen nectar their hives; this may compromise longevity life span individuals. Thus, present study aimed observe action imidacloprid in midgut ovaries Apis mellifera queens, well effects sperm stored...

The present study determined the genetic variation at mrjps loci (mrjp3, mrjp5 and mrjp8) evaluated potential use of MRJPs as molecular markers for higher royal jelly production in Africanized honeybee colonies. three analyzed produced a total 17 alleles. This high allelic polymorphism indicated these could serve markers. was by analyses multiple linear regressions with EPD (expected progeny differences) values production. variance that mrjp3 repetitive region influenced value queen's...

Tetragonisca angustula and T. fiebrigi esterases were biochemically characterized by their inhibition pattern thermostability. Workers of both species collected from nests at the State University Maringa. In three observed: EST-1 (β-esterase, cholinesterase I), EST-2 (α-esterase, II) EST-4 (αβ-esterase, carboxylesterase). two detected: EST-3 acetylesterase) e showed highest thermostability, it was not observed above 54°C, while in EST- 1 detected 52°C. Through this characterization, that...

Genetic and phenotypic parameters for hygienic behavior, invasion infestation rates, total effective reproduction of Varroa destructor in Africanized honeybee colonies producing honey (20 hives) or royal jelly (30 mini-hives) were analyzed. The significance monthly fixed effects, type product (honey jelly) their interactions verified through generalized linear model procedures. Software WinBugs (Bayesian Inference Using Gibbs Sampling) with Bayesian inference was employed (co)variance...

This research was carried out to evaluate the pollination by Africanized honeybees Apis mellifera, floral biology and observe hoarding behavior in soybean flowers (Glycine max Merril), var. BRS-133. The treatments were constituted of demarcated areas for free visitation insects, covered cages with a honeybee colony (A. mellifera) also cage without insects visitation. All had 24 m² (4m x 6m). stayed open larger time (82.82 ± 3.48 hours) area honeybees. stigma more receptive (P=0.0021) (87.3...

This research was carried out to evaluate the effect of proteic supplement on royal jelly production in honeybee colonies and its correlation with environmental variables. The treatments were: that had received a diet formulated 35% crude protein, commercial did not receive protein diet. There no difference (p > 0.05) among for parameters production. average each variable was: amount accepted larvae, 8.56 ± 6.76; acceptance percentage, 29.20 22.03%; produced per colony, 1.83 0.82 g; cup,...

In this study, performed in the western part of state Paraná, Brazil, two self-fertile hybrid commercial rapeseed genotypes were evaluated for yield components and physiological quality using three pollination tests spanning sowing dates. The treatments consisted combinations (Hyola 61 Hyola 433), (uncovered area, covered area without insects containing a single colony Africanized Apis mellifera honeybees) dates (May 25th, 2011 June 2011). presence honeybees during flowering time increased...

It is important to select the best honeybees that produce royal jelly identify molecular markers, such as major proteins (MRJPs), and hence contribute development of new breeding strategies improve production this substance.Therefore, study focused on evaluating genetic variability mrjp3, mrjp5, mrjp8 associated allele maintenance during process selective reproduction in Africanized Apis mellifera individuals, which were chosen based production.The three loci analyzed polymorphic, produced a...

Eight sunflower genotypes were analyzed to estimate correlation values for nine agronomic traits. Path analysis was used partition the into direct and indirect effects on seed yield in two pollination tests (open restricted pollination). In both tests, there significant (p<0.05) positive between head diameter, mass, number mass of achenes per first last count germination. There no (p≥0.05) 1000 weight tests. Oil content not correlated with open pollination, but it positively plants. The path...

This research was carried out to evaluate the influence of Africanized honeybees in grain production and seed quality Glycine max (L.) Merrill Roundup Ready™ transgenic soy, as well conventional soy. Transgenic soy interposed with soybean, 18 plots three treatments: covered area honeybees, without uncovered free insect visitation. The herbicide Glyphosate applied on each treatment 30 days after emergence. Grain production, number pods/plant, weight per 100 seeds, germination percentage were...

This study was carried out to evaluate the financial viability of five supplements made with different sources oil and protein, be given Africanized honeybees for royal jelly production. The verified whether investments colony supplementation give returns beekeepers, increasing profits diversification bee products. In order supplements, two experimental assays were performed. each, 20 colonies distributed randomly in four treatments, 15 replications per colony. assay I, treatments were:...

Diatraea saccharalis is the principal pest of sugarcane in Brazil and found throughout crop.Information about its population genetics scarce, but genetic analysis particular importance as a basis for successful control program.Pest requires constant evaluation variability so that appropriate strategies can be employed.In this study, structure D. populations crops was analyzed with PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) markers.Samples were collected from four...

The meliponines can be found in tropical and subtropical regions is observed the majority of Latin America. Scaptotrigona bipunctata are stingless bees that build colonies possess between 2,000 50,000 individuals. This study aimed to estimate toxicity insecticide acephate after oral contact contamination S. bipunctata. Results revealed differences two types indicated tolerant since mortality rate was low. findings there were expression changes isoenzyme esterases with insecticide, which able...

Royal jelly is an essential substance for the development of bees from larval to adult stages. Studies have identified a group key proteins in royal jelly, denominated major (MRJPs). The currently consists nine (MRJP1-MRJP9), with MRJP1 being most abundant and MRJP3 used as microsatellite marker selection queens greater production jelly. diet mostly composed proteins, supplementing this intake encourage higher their primary product important producers. It estimated that, by adding probiotic...

The sugarcane borer Diatraea saccharalis is widely known as the main pest of crop, causing increased damage to entire fields. Measures control this involve use chemicals and biological with Cotesia flavipes wasps. In study, we evaluated insecticides fipronil (Frontline; 0.0025%), malathion (Malatol Bio Carb; 0.4%), cipermetrina (Galgotrin; 10%), neem oil (Natuneem; 100%) herbicide nicosulfuron (Sanson 40 SC; in posterior region silk glands 3rd- 5th-instar D. by studying variation critical...

The migration rate of esterases and their substrate specificity for 4-methylumbelliferyl esters (acetate, propionate, butyrate) alpha- beta-naphthyl were analyzed in Diatraea saccharalis by starch gel electrophoresis. Substrate preference was observed with Est-2 Est-8 isozymes showing Est-4 isozyme butyrate alpha-naphthyl butyrate. Allele variation detected at the Est-3 locus. Two alleles, Est-3F Est-3S, identified pupae fluorogenic ester-naphthyl substrates. Chi-square analysis showed no...

This research was carried out to evaluate the pollination in siratro studying anthesis period, pollen grain viability and seed production. Two treatments were used, one covered another uncovered. The period 2.48 days 3.56 (p = 0.0001) production 8.62 11.15 seeds 0.0023), uncovered treatments, respectively. stigma receptivity test showed that 91.95% of opened flowers receptive. Pollen grains 100%. In Brazil, there are six families bees we have found five them Maringá region visiting this...