Abstract Bees are essential pollinators that contribute to maintaining biodiversity and increasing agricultural production. However, by foraging on crops, bees may become contaminated with compounds used for pest control. In this study, we exposed bee ( Apis mellifera L.) colonies the insecticide imidacloprid (IMD) under field conditions assess occurrence of oxidative stress in larvae pupae investigate morphological changes fat body midgut adult bees. The apiary area was divided into three...

The Southern region of Brazil is known for its floral diversity, presenting great potential bee products such as honey, pollen, propolis and royal jelly. In this study, 67 honey samples provided by beekeepers from 14 municipalities western Paraná were evaluated. Physicochemical parameters, bioactive compounds antioxidant activity analyzed. physicochemical parameters the in compliance with specifications national international standards, average values 3.26, 34.54 meq.kg-1, 18.75%, 10.79...

Bee colony health is declining as a result of several factors, including exposure to pesticides. The development and strength honey bee colonies depend on the reproductive success queen bees. Because flowers are sources food for bees, foragers can accidentally collect carry contaminated pollen nectar their hives; this may compromise longevity life span individuals. Thus, present study aimed observe action imidacloprid in midgut ovaries Apis mellifera queens, well effects sperm stored...

A melissopalinologia foi utilizada para determinar a origem botânica do mel, importante sua rastreabilidade. Quarenta amostras de mel dos municípios Santa Helena (20) e Terra Roxa (20), região oeste Paraná, foram analisadas acordo com os tipos pólen comparações catálogos literatura especializada. Em relação à qualidade analítica, identificados 300 grãos por amostra. Nas Helena, encontrados 71 24 famílias, classificados como monofloral Hovenia dulcis (8) o restante multifloral (12). da Roxa,...

Antimicrobial activity of honey has lately drawn researchers’ attention, especially due to the fact that its antimicrobial capacity been proven. The aim this study was analyzing bactericidal and bacteriostatic action in pathogenic microorganisms Staphylococcus aureus, Escherichia coli Candida albicans samples from five regions state Paraná. yeast showed be more resistant than bacteria aureus. minimal inhibitory concentration found for three tested 12.5% 25%. concentration, turn, 25% 50%...

Objetivou-se avaliar o efeito antiparasitário de diferentes dosagens do extrato alcoólico da própolis no controle helmintos gastrointestinais em ovinos Santa Inês. Foram utilizadas 32 ovelhas raça Inês, distribuídas delineamento experimental blocos completos casualizados, com quatro tratamentos e oito repetições. Os foram: 10 mL água; álcool 70%; 5 (EAP) a 30% EAP 30%. foram administrados por via oral, dose única. efeitos dos avaliados pela contagem ovos gramas fezes (OPG), referentes aos 1,...

The stingless bee Tetragonisca angustula is an important pollinator of different agricultural and native crops. This study evaluated changes in the relative activity esterases critical electrolyte concentration brain cells after exposure to pesticides malathion thiamethoxam. Lethal 50% showed greater toxicity thiamethoxam relation malathion. Esterases EST-3 EST-4 (carboxylesterase) were partially inhibited contamination by contact ingestion with thiamethoxam, suggesting participation these...

Tetragonisca angustula is the stingless bee more managed by beekeepers, whose honey has many medicinal properties and distributed throughout Latin America. They have variation in color of mesepisternum can be black, yellow or mixed. might classified into two species (T. T. fiebrigi) subspecies fiebrigi). This study aimed was to analyze genetic variability populations sampled using technique simple repeated sequences interaction molecular markers used other studies verify possible existence a...

Stingless bees are important pollinators for various plant crops. We investigated the susceptibility of Tetragonisca fiebrigi to sublethal concentrations insecticides fipronil, malathion, and thiamethoxam (administered through contact ingestion) by determining LC50 values after 24 hours exposure analyzing changes in activity esterase isoenzymes chromatin brain cells. The showed that all three were highly toxic ingestion. Electrophoretic analysis revealed relative EST-4 (carboxylesterase) T....

The objective of this work was to evaluate the physicochemical characteristics, bioactive compounds and antioxidant potential honey produced by Africanized bees (Apis mellifera L.) from municipalities in western Paraná state. Sixty-six samples were analyzed October 2018 August 2019. parameters evaluated were: color, moisture, hydroxymethylfurfural, pH free acidity, with mean values ​​of 0.36±0.24nm, 18 .80±1.01%, 10.34±3.24mg.kg-1, 4.01±0.18 17.36±4.11 meq.kg-1, respectively. (phenolic...

This research was carried out to evaluate the foraging behaviour of Apis mellifera and Scaptotrigona bipunctata its relationship with environmental variables in Dombeya wallichii considering following aspects: 1) Type resource harvested, 2) Visitation rate, 3) Length visit, 4) sugar concentration stored nectar honey crop A. secreted by flower 5) floral constancy. Both species collected mainly nectar, a visitation rate 4.2 flowers for 1.1 S. visit length 9.2 34.2 seconds, respectively. The...

Abstract Honey from the western region of Paraná receives seal origin and in-process denomination because it is mostly produced in permanent preservation areas, adherence to hygiene sanitary standards, proper management techniques agro-industrial management. The objective this study was verify microbiological quality honey by beekeepers associated with Cooperative. Sixty-seven samples were evaluated for quality, mean values observed total aerobic mesophiles 2.52 log CFU/g; Clostridium spp....

Stingless bees Tetragonisca angustula (Latreille) (Hymenoptera: Meliponinae) are pollinators of native and cultivated plants, therefore in contact with areas contaminated by pesticides. These were evaluated for changes gene expression esterase isoenzymes peptides after contamination insecticides growth regulators Gallaxy ® EC 100, Natuneem Azamax 48, 120, 168 hours, 30 60 days. EST-4 presented an increase relative activity 100 at 6.2 × 10-2 g a.i/mL, 7.5 10-5 a.i/mL 1.2 10-3 days, 48 h...

This study evaluated silages made with varying proportions of viticulture by-products (VC) and starch extraction from cassava (CSE). It attempted to determine the effects these on microbial population, fermentative losses, chemical composition. The treatments were specified as VC in silage (0 g/kg, 250 500 750 1000 g/kg). Silages before (0) after 1, 3, 7, 15, 30, 60 days ensiling. experimental design was completely randomized five treatments, six storage times four replications. increased...

The potential of royal jelly production in Africanized honeybee colonies was evaluated using techniques involving two queens per colony horizontal and vertical systems during the spring 2019. were tested for their effect on cell acceptance (%); (g), harvest position bar (g) compared with standard (five ten-frame hives) southern Brazil. Results showed statistical significance between methods. Vertical [...]

The meliponinae are important pollinators of plant species. T. weyrauchi has restricted distribution , is found only in northern Brazil . This study aimed to perform the electrophoretic characterization esterases from and check expression pattern total proteins. Adult individuals were collected at entrance nests located Alto Paraiso, state Rondonia, stored -20 °C. Individual extracts head thorax abdomen workers subjected electrophoresis on 10% polyacrylamide gel 5% stacking gel. Extracts...

La suplementación alimentaria de las colonias abejas melíferas Apis mellifera es, junto con el manejo y la cría genética, una bases para alta productividad en apicultura. Sin embargo, equilibrio adecuado nutrientes que componen dieta es esencial tenga beneficios productivos y, consecuencia, ganancias del apicultor. Los juegan un papel clave variedad funciones fisiológicas los individuos colonia, incluyendo reproducción, crecimiento, desarrollo, producción supervivencia durante invierno....

Abstract The objective was to evaluate the behavior of Apis mellifera and Tetragonisca angustula bees in pollination tests Brassica napus at different times day, temperature relative humidity. experimental design completely with eight treatments two tests, repeated five randomized blocks during seven days observations for years, totaling 560 records. During visits, following parameters were recorded: collected resources, nectar collection site, time spent on flower, number flowers visited...