A5012694357- Mosquito-borne diseases and control
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Malaria Research and Control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Leprosy Research and Treatment
- Invertebrate Immune Response Mechanisms
- Viral Infections and Vectors
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Immunodeficiency and Autoimmune Disorders
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- RNA regulation and disease
- Protein Structure and Dynamics
- Infectious Diseases and Tuberculosis
- Neurobiology and Insect Physiology Research
- Microbial Fuel Cells and Bioremediation
- Evolution and Genetic Dynamics
- Iron Metabolism and Disorders
- Vibrio bacteria research studies
- Immune responses and vaccinations
Institut Pasteur
2012-2022
Centre National de la Recherche Scientifique
2011-2022
Modèles Insectes de l'Immunité Innée
2015-2022
Université Paris Cité
2022
European Bioinformatics Institute
2007
Wellcome Trust
2007
Broad Institute
2007
Virginia Tech
2007
Johns Hopkins University
2007
Wellcome Sanger Institute
2001-2003
The distribution of 20 variable regions resulting from insertion-deletion events in the genomes tubercle bacilli has been evaluated a total 100 strains Mycobacterium tuberculosis , africanum canettii microti and bovis . This approach showed that majority these polymorphisms did not occur independently different M. complex but, rather, resulted ancient, irreversible genetic common progenitor strains. Based on presence or absence an specific deletion (TbD1), tuberculosi s can be divided into...
We present a draft sequence of the genome Aedes aegypti, primary vector for yellow fever and dengue fever, which at approximately 1376 million base pairs is about 5 times size malaria Anopheles gambiae. Nearly 50% Ae. aegypti consists transposable elements. These contribute to factor 4 6 increase in average gene length sizes intergenic regions relative An. gambiae Drosophila melanogaster. Nonetheless, chromosomal synteny generally maintained among all three insects, although conservation...
Mycobacterium bovis is the causative agent of tuberculosis in a range animal species and man, with worldwide annual losses to agriculture $3 billion. The human burden caused by bovine tubercle bacillus still largely unknown. M. was also progenitor for Calmette–Guérin vaccine strain, most widely used vaccine. Here we describe 4,345,492-bp genome sequence AF2122/97 its comparison genomes leprae . Strikingly, >99.95% identical that , but deletion genetic information has led reduced size....
To understand the evolution, attenuation, and variable protective efficacy of bacillus Calmette-Guérin (BCG) vaccines, Mycobacterium bovis BCG Pasteur 1173P2 has been subjected to comparative genome transcriptome analysis. The 4,374,522-bp contains 3,954 protein-coding genes, 58 which are present in two copies as a result independent tandem duplications, DU1 DU2. is restricted Pasteur, although four forms DU2 exist; DU2-I confined early like Japan, whereas DU2-III DU2-IV occur late vaccines....
Whole‐genome comparisons of the tubercle bacilli were undertaken using ordered bacterial artificial chromosome (BAC) libraries Mycobacterium tuberculosis and vaccine strain, bovis BCG‐Pasteur, together with complete genome sequence M. H37Rv. Restriction‐digested BAC arrays H37Rv used in hybridization experiments radiolabelled BCG genomic DNA to reveal presence 10 deletions (RD1–RD10) relative . Seven these regions, RD4–RD10, also found be deleted from , three BCG‐specific being identical...
An integrated map of the genome tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra to be positioned on 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs means fingerprinting hybridization mapping. The resultant contig readily correlated with physical via landmarked restriction sites. Over 165 genes markers...
ABSTRACT The bacterial artificial chromosome (BAC) cloning system is capable of stably propagating large, complex DNA inserts in Escherichia coli . As part the Mycobacterium tuberculosis H37Rv genome sequencing project, a BAC library was constructed pBeloBAC11 vector and used for mapping, confirmation sequence assembly, sequencing. contains about 5,000 clones, with ranging size from 25 to 104 kb, representing theoretically 70-fold coverage M. (4.4 Mb). A total 840 sequences T7 SP6 termini...
Collecting mosquito larvae from West African ponds has revealed a previously unknown but highly abundant genotype.
Abstract Background Anopheles stephensi is the key vector of malaria throughout Indian subcontinent and Middle East an emerging model for molecular genetic studies mosquito-parasite interactions. The type form species responsible majority urban transmission across its range. Results Here, we report genome sequence annotation strain An. . 221 Mb assembly represents more than 92% entire was produced using a combination 454, Illumina, PacBio sequencing. Physical mapping assigned 62% onto...
Mycobacterium microti is a member of the tuberculosis complex that causes in voles. Most strains M. are harmless for humans, and some have been successfully used as live vaccines. In an attempt to identify putative virulence factors tubercle bacilli, genes absent from avirulent but present human pathogen or bovis were searched for. A minimal set 50 bacterial artificial chromosome (BAC) clones covers almost all genome OV254 was constructed, individual BACs compared corresponding AF2122/97...
In enteric bacteria, the expression of many genes encoding various anaerobic electron transfer functions is controlled by FNR, product autoregulated fnr gene. FNR structurally and functionally homologous to CAP, catabolite gene activator protein, increased production strongly stimulates transcription its target genes. By analysis RNA produced in vivo promoters four FNR-dependent were localized shown display a common arrangement. A 22bp dyad symmetry was found about 30 nucleotides upstream...
In the Greater Mekong Subregion, malaria cases have significantly decreased but little is known about vectors or mechanisms responsible for residual transmission. We analysed a total of 3920 Anopheles mosquitoes collected during rainy and dry seasons from four ecological settings in Cambodia (villages, forested areas near villages, rubber tree plantations forest sites). Using odor-baited traps, 81% samples across all sites were cow baited although 67% attracted by human traps sites. Overall,...
Summary In an attempt to unify the genetic and biological research on Mycobacterium leprae , aetiological agent of leprosy, a cosmid library was constructed then ordered by combination fingerprinting hybridization techniques. The genome M. is represented four contigs overlapping clones which, together, account for nearly 2.B Mb DNA. Several arguments suggest that gaps between are small in size virtually complete coverage chromosome has been obtained. All cloned genes have positioned contig...
The nucleotide sequence of a 4.8-kilobase SacII-PstI fragment encoding the anaerobic glycerol-3-phosphate dehydrogenase operon Escherichia coli has been determined. consists three open reading frames, glpABC, polypeptides molecular weight 62,000, 43,000, and 44,000, respectively. 62,000- 43,000-dalton subunits corresponded to catalytic GlpAB dimer. larger GlpA subunit contained putative flavin adenine dinucleotide-binding site, smaller GlpB possible mononucleotide-binding domain. GlpC two...
Everything that we need to know about Mycobacterium leprae, a close relative of the tubercle bacillus, is encrypted in its genome. Inspection 3.27 Mb genome sequence an armadillo-derived Indian isolate leprosy bacillus identified 1,605 genes encoding proteins and 50 for stable RNA species. Comparison with tuberculosis revealed extreme case reductive evolution, since less than half contains functional while inactivated or pseudogenes are highly abundant. The level gene duplication was...
Effective protection against a virulent challenge with Mycobacterium tuberculosis is induced mainly by previous immunization living attenuated mycobacteria, and it has been hypothesized that secreted proteins serve as major targets in the specific immune response. To identify purify molecules present culture medium filtrate which are dominant antigens during effective vaccination, two-step selection procedure was used to select able interact T lymphocytes and/or antibodies bacteria...
Summary The nucleotide sequences of the glpT gene Escherichia coli and its regulatory region have been elucidated primary structure glycerot‐3‐phosphate transport protein deduced. Extensive amino acid sequence homology was found with two other cytoplasmic membrane proteins: functionally related hexose‐6‐phosphate protein, UHPC involved in regulating uptake. Although no significant proteins, such as arabinose, citrate, glucose, melibiose, lactose or xylose transporters, all these proteins...
Members of the Anopheles gambiae species complex are primary vectors human malaria in Africa. It is known that a large haplotype shared between An. and coluzzii by introgression carries point mutations voltage-gated sodium channel gene para, including L1014F kdr mutation associated with insensitivity to pyrethroid insecticides. Carriage also correlated higher susceptibility infection Plasmodium falciparum. However, genetic mechanism causative gene(s) underlying parasite phenotype not known....
Abstract Background The Anopheles gambiae sensu lato (s.l.) species complex in Burkina Faso consists of arabiensis, and molecular forms M S stricto (s.s.). Previous studies comparing the for level infection with Plasmodium falciparum have yielded conflicting results. Methods Mosquito larvae were sampled from natural pools, reared to adulthood under controlled conditions, challenged P. by experimental feeding blood gametocyte carriers. Oocyst prevalence intensity was determined one week after...
Mosquitoes of the Anopheles gambiae complex display strong preference for human bloodmeals and are major malaria vectors in Africa. However, their interaction with viruses or role arbovirus transmission during epidemics has been little examined, exception O'nyong-nyong virus, closely related to Chikungunya virus. Deep-sequencing revealed different RNA natural insect viromes, but none have previously described species complex. Here, we describe two novel viruses, a Dicistrovirus Cypovirus,...