A5056585486- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Insect Utilization and Effects
- MicroRNA in disease regulation
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
- Cancer-related molecular mechanisms research
- Antioxidant Activity and Oxidative Stress
- Insect symbiosis and bacterial influences
- Circadian rhythm and melatonin
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Animal Behavior and Reproduction
- Extracellular vesicles in disease
- Microbial Community Ecology and Physiology
- Airway Management and Intubation Techniques
- Viral Infections and Vectors
- Medicinal Plant Pharmacodynamics Research
- Bone and Joint Diseases
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
People's Hospital of Cangzhou
2011-2025
Virginia Tech
2013-2024
Affiliated Hospital of Guizhou Medical University
2024
Zunyi Medical University
2024
Hebei University
2018
Affiliated Hospital of Zunyi Medical College
2002-2017
Second Affiliated Hospital of Soochow University
2013-2017
Soochow University
2013-2017
First Affiliated Hospital of Wannan Medical College
2016
Wannan Medical College
2016
We present a draft sequence of the genome Aedes aegypti, primary vector for yellow fever and dengue fever, which at approximately 1376 million base pairs is about 5 times size malaria Anopheles gambiae. Nearly 50% Ae. aegypti consists transposable elements. These contribute to factor 4 6 increase in average gene length sizes intergenic regions relative An. gambiae Drosophila melanogaster. Nonetheless, chromosomal synteny generally maintained among all three insects, although conservation...
Juvenile hormone (JH) plays crucial roles in many aspects of insect life. The Methoprene-tolerant ( Met ) gene product, a member the bHLH-PAS family transcriptional regulators, has been demonstrated to be key component JH signaling pathway. However, molecular function JH-induced signal transduction and regulation remains fully elucidated. Here we show that coactivator ecdysteroid receptor complex, FISC, acts as functional partner mediating expression. FISC appear use their PAS domains form...
Abstract Life experience can leave lasting marks, such as epigenetic changes, in the brain. How life is translated into storable information remains largely unknown. With unbiased data-driven approaches, we predicted that Egr1 , a transcription factor important for memory formation, plays an essential role brain programming. We performed EGR1 ChIP-seq and validated thousands of binding sites with methylation patterns established during postnatal development. More specifically, these become...
Significance Juvenile hormone (JH) controls many key processes during insect life cycles. Some of the effects JH are mediated by a membrane-associated mechanism. In other circumstances, an intracellular receptor, methoprene-tolerant protein (MET), is activated upon binding and directly regulates expression target genes. Here we use adult female mosquitoes as example to demonstrate that both mechanisms interconnected coordinate hormonal responses. Our results indicate phospholipase C pathway...
In anautogenous mosquitoes, vitellogenesis, which includes production of yolk protein precursors, requires blood feeding. Consequently, mosquitoes transmit many diseases. Understanding the molecular mechanisms vitellogenesis regulation will contribute significantly to vector control strategies. Newly emerged Aedes aegypti females require 3 days before becoming competent activate in response a blood-meal-initiated, elevated titer 20-hydroxyecdysone (20E). An orphan nuclear receptor gene β...
Hormones provide generalized signals that are interpreted in a specific spatial and temporal manner by developing or reproducing multicellular organism. The ability to respond hormones is determined the competence of cell tissue. betaFtz-F1 orphan nuclear receptor acts as factor for steroid hormone 20-hydroxyecdysone (20E) Drosophila melanogaster metamorphosis mosquito reproduction. molecular nature action remains unclear. We report protein-protein interaction between p160/SRC coactivator...
Abstract Icaritin, a small molecule currently being investigated in phase III clinical trials China (NCT03236636 and NCT03236649) for treatment of advanced hepatocellular carcinoma (HCC), is prenylflavonoid derivative obtained from the Epimedium genus . Previously, it was found that Icaritin decreased expression PD‐L1, but its direct molecular targets underlying mechanisms have not been identified. In this study, we report identification IKK‐α as protein target by biotin‐based affinity...
Abstract Non‐small cell lung cancer (NSCLC) is a primary contributor to global cancer‐related mortality. Musashi‐2 (MSI2), an RNA‐binding protein (RBP), upregulated in specific NSCLC tumor subgroups. The current investigation evaluated the role and underlying mechanism of MSI2 NSCLC. expression levels ELK4, MSI2, SMAD3, p‐SMAD3 TGF β R1 were assessed via RT–qPCR or Western blot. Chromatin immunoprecipitation (ChIP) dual luciferase reporter assays used confirm interaction between ELK4 MSI2....
The broad ( br ) gene, encoding a family of C2H2 type zinc-finger DNA-binding proteins, has been shown to act as crucial member the 20-hydroxyecdysone (20E) regulatory hierarchy in fruitfly, Drosophila melanogaster and moth, Manduca sexta . In this study, we have that gene is involved 20E-regulatory controlling vitellogenesis mosquito, Aedes aegypti Unlike E74 E75 early genes, expression was activated previtellogenic females, during juvenile hormone (JH)-dependent period. levels Z1, Z2 Z4...
Abstract Krüppel homologue 1 (Kr‐h1) is a zinc finger transcription factor that upregulated in insects by juvenile hormone (JH) metamorphosis and adult reproduction. The molecular function of Kr‐h1 reproduction remains largely unknown. Here we report AaKr‐h1 functions as an important regulator female Aedes aegypti mosquitoes. amount protein increases with rising JH levels after emergence, reaches its peak at 48 h eclosion, then decreases gradually disappears blood feeding. RNA interference...
Significance Many physiological events in insects are controlled by both juvenile hormone (JH) and 20-hydroxyecdysone (20E). The presence of JH sometimes alters the nature 20E action, but underlying mechanisms have not been fully elucidated. In Aedes aegypti mosquitoes, four protein isoforms generated taiman gene, which plays an indispensable role action 20E. Our study indicates that different responses demand distinct Taiman isoforms. Moreover, controls formation specifically required for...
In anautogenous mosquitoes, egg maturation requires a blood meal. As consequence, mosquitoes are vectors of numerous devastating human diseases. Blood feeding triggers 20-hydroxyecdysone (20E) hormonal cascade, which activates yolk protein precursor ( YPP ) genes in the female fat body, an insect metabolic tissue. An important adaptation for anautogeny is previtellogenic arrest preventing activation genes. Equally essential termination their expression, so that another achieved after batch...