A5100639759- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Insect Utilization and Effects
- Entomopathogenic Microorganisms in Pest Control
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Silkworms and Sericulture Research
- Insect and Arachnid Ecology and Behavior
- African Botany and Ecology Studies
- Otolaryngology and Infectious Diseases
- Antimicrobial Peptides and Activities
- Head and Neck Anomalies
- Viral Infectious Diseases and Gene Expression in Insects
- Agricultural pest management studies
- Insect Pest Control Strategies
- Remote Sensing and Land Use
- Silk-based biomaterials and applications
- Remote Sensing in Agriculture
- Viral Infections and Vectors
- Leaf Properties and Growth Measurement
- CRISPR and Genetic Engineering
- interferon and immune responses
- Plant pathogens and resistance mechanisms
- Aquaculture disease management and microbiota
- Mosquito-borne diseases and control
Center for Excellence in Molecular Plant Sciences
2016-2024
Chinese Academy of Sciences
2016-2024
Shanghai Institutes for Biological Sciences
2016-2017
Environment and Plant Protection Research Institute
2016
Chinese Academy of Tropical Agricultural Sciences
2016
Skin immunity protects animals from airborne pathogen infection. Unlike mammals, arthropods, including insects, undergo periodic ecdysis to grow and develop. Newly-molted insects emerge with un-sclerotized thin cuticles but successfully escape pathogenic infections during the post-molt period. Here we show that prophenoloxidases in molting fluids remain bioactive on integument impede fungal infection after ecdysis. We found purified plasma or recombinant could effectively bind spores...
Insects can be models for understanding human intestinal infection and pathology. Molting, a special period during which the old insect cuticle is shed new one produced, crucial development. Holometabolous insects may experience several larva-to-larva moltings to become larger, pupal molt adult eclosion adults. During larval molts, they stop feeding quiescent. Although molting larvae quiescent, it not known if changes in microbiome, physiology, development immunity of midguts...
Insects depend on the innate immune response for defense against a wide array of pathogens. Central to Drosophila immunity are antimicrobial peptides (AMPs), released into circulation when pathogens trigger either two widely studied signal pathways, Toll or IMD. The pathway responds infection by Gram-positive bacteria and fungi while IMD is activated Gram-negative bacteria. During activation pathway, NF-κB-like transcription factor Relish phosphorylated then cleaved, which crucial...
Abstract Invertebrate animals have the capacity of repairing wounds in skin and gut via different mechanisms. Gastrointestinal perforation, a hole human gastrointestinal system, is serious condition surgery necessary to repair perforation prevent an abdominal abscess or sepsis. Here we report made by needle-puncture wound silkworm larval midgut. Following insect only weak immune response was observed because growth Escherichia coli alone partially inhibited plasma collected at 6 h after...
The insect cuticle works as the first line of defence to protect insects from pathogenic infections and water evaporation. However, old must be shed in order enter next developmental stage. During each ecdysis, moulting fluids are produced secreted into area among new cuticles. In a previous study, protein Bombyx mori single domain von Willebrand factor type C (BmSVWC; BGIBMGA011399) was identified Bo. demonstrated regulate ecdysis. this study we show that larvae, BmSVWC primarily locates...
Insect prophenoloxidase (PPO) induces melanization around pathogens. Before melanization, PPO is cleaved into phenoloxidase (PO) by serine proteases. can also be activated exogenous proteases secreted pathogens as well other compounds, such ethanol and cetylpyridinium chloride (CPC). However, the effect of these activators on activity PO unclear. In this study, insect endogenous protease AMM1, α-chymotrypsin, were used to activate recombinant Drosophila PPO1 (rPPO1), differed depending...
Entomopathogenic fungi Beauveria bassiana can infect many species of insects and is used as a biological pesticide world-wide. Before reaching the hemocoel, B. has to penetrate integument which composed thick chitin layer epidermal cells. Some chitinase, protease lipase secreted by are probably involved in fungal penetration integument. While microscopic proof needed, it difficult locate precise infection sites following traditional method immersion infection. Consequently, we developed new...
Abstract Signal peptidase complexes (SPCs) are conserved from bacteria to human beings, and typically composed of four five subunits. There genes encoding SPC proteins in the red flour beetle, Tribolium castaneum . To understand their importance insect development, double‐stranded RNA for each gene was injected into beetles at early larval adult stages. Knockdown all signal lethal larvae. Moreover, larvae had difficulty with old cuticle ecdysis. TcSPC12 alone did not affect pupal or...
Integuments are the first line to protect insects from physical damage and pathogenic infection. In lepidopteran insects, they undergo distinct morphology changes such as scale formation during metamorphosis. However, we know little about integument development this stage. Here, use silkworm, Bombyx mori, a model show that stem cells in of each segment, but not intersegmental membrane, divide into two precursor spinning young pupae, cell divides again. One daughter becomes mature...
Maintaining a definite and stable pool of dividing stem cells plays an important role in organ development. This process requires appropriate progression mitosis for proper spindle orientation polarity to ensure the ability proliferate differentiate correctly. Polo-like kinases (Plks)/Polo are highly conserved serine/threonine involved initiation as well cell cycle. Although numerous studies have investigated mitotic defects upon loss Plks/Polo cells, little is known about vivo consequences...