A5077610154- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Resistance
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Heat shock proteins research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant and fungal interactions
- Plant and Fungal Interactions Research
- Ubiquitin and proteasome pathways
- Polysaccharides and Plant Cell Walls
Netherlands Institute for Neuroscience
2008-2025
University of Amsterdam
2016-2025
Henan University
2022
Savitribai Phule Pune University
2022
Central South University of Forestry and Technology
2022
Central South University
2022
University of Oxford
2022
Norwich Research Park
2022
John Innes Centre
2022
Friedrich Schiller University Jena
2022
Resistance (R) proteins in plants are involved pathogen recognition and subsequent activation of innate immune responses. Most resistance contain a central nucleotide-binding domain. This so-called NB-ARC domain consists three subdomains: NB, ARC1, ARC2. The is functional ATPase domain, its state proposed to regulate activity the R protein. A highly conserved methionine-histidine-aspartate (MHD) motif present at carboxy-terminus An extensive mutational analysis MHD I-2 Mi-1 reported. Several...
Most plant disease resistance (R) genes known today encode proteins with a central nucleotide binding site (NBS) and C-terminal Leu-rich repeat (LRR) domain. The NBS contains three ATP/GTP motifs as the kinase-1a or P-loop, kinase-2, kinase-3a motifs. In this article, we show that of R forms functional pocket. N-terminal halves two tomato proteins, I-2 conferring to Fusarium oxysporum Mi-1 root-knot nematodes potato aphids, were produced glutathione S-transferase fusions in Escherichia coli....
To promote host colonization, many plant pathogens secrete effector proteins that either suppress or counteract defences. However, when these effectors are recognized by the host's innate immune system, they trigger resistance rather than promoting virulence. Effectors therefore key molecules in determining disease susceptibility resistance. We show here Avr2, secreted vascular wilt fungus Fusarium oxysporum f. sp. lycopersici (Fol), shows both activities: it is required for full virulence a...
Plant intracellular immune receptors comprise a large number of multi-domain proteins resembling animal NOD-like (NLRs). NLRs typically recognize isolate-specific pathogen-derived effectors, encoded by avirulence (AVR) genes, and trigger defense responses often associated with localized host cell death. The barley MLA gene is polymorphic in nature encodes the coiled-coil (CC)-NB-LRR type that each detects cognate effector powdery mildew fungus. We report systematic analyses MLA10 activity...
Abstract Background The plant-pathogenic fungus Fusarium oxysporum f.sp .lycopersici ( Fol ) has accessory, lineage-specific (LS) chromosomes that can be transferred horizontally between strains. A single LS chromosome in the Fol4287 reference strain harbors all known effector genes. Transfer of this pathogenicity confers virulence to a previously non-pathogenic recipient strain. We hypothesize expression and evolution genes is influenced by their genomic context. Results To gain better...
Abstract Resistance (R) proteins in plants confer specificity to the innate immune system. Most R have a centrally located NB-ARC (nucleotide-binding adaptor shared by APAF-1, proteins, and CED-4) domain. For two tomato (Lycopersicon esculentum) I-2 Mi-1, we previously shown that this domain acts as an ATPase module can hydrolyze ATP vitro. To investigate role of nucleotide binding hydrolysis for function planta, specific mutations were introduced conserved motifs Two resulted autoactivating...
The phytopathogenic fungus Alternaria alternata f. sp. lycopersici (AAL) produces toxins that are essential for pathogenicity of the on tomato ( Lycopersicon esculentum ). AAL and fumonisins unrelated Fusarium moniliforme sphinganine-analog mycotoxins (SAMs), which cause inhibition sphingolipid biosynthesis in vitro toxic some plant species mammalian cell lines. Sphingolipids can be determinants proliferation or death cells. We investigated A lternaria s tem c anker Asc ) locus, mediates...
Summary Tomato ( Solanum lycopersicum ) Cf resistance genes confer hypersensitive response (HR)‐associated to strains of the pathogenic fungus Cladosporium fulvum that express matching avirulence Avr gene. Previously, we identified an A vr4 ‐ r esponsive t omato (ART) gene is required for Cf‐4 / Avr4 ‐induced HR in Nicotiana benthamiana as demonstrated by virus‐induced silencing (VIGS). The encodes a CC‐NB‐LRR type (R) protein analogue have designated NRC1 N B‐LRR equired HR‐associated c ell...
To intercept invading microbes that threaten growth and reproduction, plants evolved a sophisticated innate immune system. Recognition of specialized pathogens is mediated by resistance proteins function as molecular switches. Pathogen perception these multidomain seems to trigger series conformational changes dependent on nucleotide exchange. The activated protein switches host defenses, often culminating in the death infected cells. Given their control over life death, activity requires...
Abstract Virus-induced gene silencing identified the Avr9/Cf-9 RAPIDLY ELICITED ACRE189 as essential for Cf-9– and Cf-4–mediated hypersensitive response (HR) in Nicotiana benthamiana. We report a role disease resistance tomato (Solanum lycopersicum) tobacco (Nicotiana tabacum). (herein renamed Avr9/Cf-9–INDUCED F-BOX1 [ACIF1]) encodes an F-box protein with Leu-rich-repeat domain. ACIF1 is widely conserved closely related to proteins regulating plant hormone signaling. Silencing of suppressed...
Abstract Posttranslational modifications allow dynamic and reversible changes to protein function. In Arabidopsis thaliana, a small gene family encodes paralogs of the ubiquitin-like posttranslational modifier. We studied function these paralogs. Single mutants SUM1 SUM2 do not exhibit clear phenotype. However, corresponding double knockdown mutant revealed that are essential for plant development, floral transition, suppression salicylic acid (SA)–dependent defense responses. The genes...
Plant pathogens secrete effectors to manipulate their host and facilitate colonization. Fusarium oxysporum f. sp. lycopersici is the causal agent of wilt disease in tomato. Upon infection, F. secretes numerous small proteins into xylem sap (Six proteins). Most Six are unique oxysporum, but Six6 an exception; a homolog also present two Colletotrichum spp. SIX6 expression was found require living cells knockout compromised virulence, classifying it as genuine effector. Heterologous did not...
Summary Plant‐invading microbes betray their presence to a plant by exposure of antigenic molecules such as small, secreted proteins called ‘effectors’. In Fusarium oxysporum f. sp . lycopersici ( Fol ) we identified pair effector gene candidates, AVR 2 ‐ SIX 5 , whose expression is controlled shared promoter. The pathogenicity and knockouts was assessed on susceptible resistant tomato Solanum lycopersicum plants carrying I‐2 NB LRR protein confers resistance races Like Avr2, Six5 found be...
Summary Phytohormones, such as salicylic acid (SA), ethylene (ET) and jasmonic (JA), play key roles in plant defence following pathogen attack. The involvement of these hormones susceptibility Fusarium oxysporum ( Fo ) infection has mostly been studied Arabidopsis thaliana . However, causes vascular wilt disease a broad range crops, including tomato Solanum lycopersicum ). Surprisingly little is known about the phytohormones towards f. sp. lycopersici Fol Here, we investigate their by...
Pathogens use effector proteins to manipulate their hosts. During infection of tomato, the fungus Fusarium oxysporum secretes effectors Avr2 and Six5. Whereas suffices trigger I-2-mediated cell death in heterologous systems, both are required for disease resistance tomato. How Six5 participates triggering is unknown. Using bimolecular fluorescence complementation assays we found that interact at plasmodesmata. Single-cell transformation revealed a 2xRFP marker protein Avr2-GFP only move...