A5076291199- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Mycotoxins in Agriculture and Food
- Plant pathogens and resistance mechanisms
- Fungal Plant Pathogen Control
- Plant Pathogens and Resistance
- Fungal Biology and Applications
- Wheat and Barley Genetics and Pathology
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Essential Oils and Antimicrobial Activity
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
- Biomedical Text Mining and Ontologies
- Plant Molecular Biology Research
- Mycorrhizal Fungi and Plant Interactions
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
Hebei Agricultural University
2018-2025
Australian National University
2019-2025
Harbin University of Science and Technology
2023-2024
Southwest University
2020-2022
Agriculture and Agri-Food Canada
2013-2022
University of Amsterdam
2011-2016
Netherlands Institute for Neuroscience
2013
Beijing Forestry University
2009
Tokyo Medical and Dental University
1993
To promote host colonization, many plant pathogens secrete effector proteins that either suppress or counteract defences. However, when these effectors are recognized by the host's innate immune system, they trigger resistance rather than promoting virulence. Effectors therefore key molecules in determining disease susceptibility resistance. We show here Avr2, secreted vascular wilt fungus Fusarium oxysporum f. sp. lycopersici (Fol), shows both activities: it is required for full virulence a...
Abstract Background The plant-pathogenic fungus Fusarium oxysporum f.sp .lycopersici ( Fol ) has accessory, lineage-specific (LS) chromosomes that can be transferred horizontally between strains. A single LS chromosome in the Fol4287 reference strain harbors all known effector genes. Transfer of this pathogenicity confers virulence to a previously non-pathogenic recipient strain. We hypothesize expression and evolution genes is influenced by their genomic context. Results To gain better...
Summary Leucine‐rich repeat receptor‐like proteins ( LRR ‐ RLP s) are highly adaptable parts of the signalling apparatus for extracellular detection plant pathogens. Resistance to blackleg disease B rassica spp. caused by L eptosphaeria maculans is largely governed host race‐specific R ‐genes, including gene LepR3 . The resistance Rlm2 was previously mapped same genetic interval as In this study, locus napus line ‘ G lacier DH 24287’ cloned, and transformants were analysed recovery...
Summary Plant‐invading microbes betray their presence to a plant by exposure of antigenic molecules such as small, secreted proteins called ‘effectors’. In Fusarium oxysporum f. sp . lycopersici ( Fol ) we identified pair effector gene candidates, AVR 2 ‐ SIX 5 , whose expression is controlled shared promoter. The pathogenicity and knockouts was assessed on susceptible resistant tomato Solanum lycopersicum plants carrying I‐2 NB LRR protein confers resistance races Like Avr2, Six5 found be...
Plants have evolved multifaceted defence mechanisms to resist pathogen infection. Production of the pathogenesis-related (PR) proteins in response attack has been implicated plant disease resistance specialized systemic-acquired (SAR). Our earlier studies reported that a full length TaLr35PR5 gene, encoding protein exhibiting amino acid and structural similarity sweet thaumatin, was isolated from wheat near-isogenic line TcLr35. The present study aims understand function gene Lr35-mediated...
Blackleg disease of Brassica napus caused by Leptosphaeria maculans (Lm) is largely controlled the deployment race-specific resistance (R) genes. However, selection pressure exerted R genes causes Lm to adapt and give rise new virulent strains through mutation deletion effector Therefore, a knowledge gene function necessary for effective management disease. Here, we report cloning AvrLm9 which recognized Rlm9 in B. cultivar Goéland. was mapped scaffold 7 genome, co-segregating with...
SUMMARY In plants, race‐specific defence against microbial pathogens is facilitated by resistance ( R ) genes which correspond to specific pathogen avirulence genes. This study reports the cloning of a blackleg gene from Brassica napus (canola), Rlm9 , encodes wall‐associated kinase‐like (WAKL) protein, newly discovered class plant RLK provides isolates Leptosphaeria maculans carrying corresponding AvrLm5‐9 representing only second WAKL‐type described date. The protein predicted be cell...
Plant pathogens secrete proteins, known as effectors, that function in the apoplast or inside plant cells to promote virulence. Effector recognition by cell-surface cytosolic receptors results activation of defence pathways and immunity. Despite their importance, our general understanding fungal effector immunity remains poor. One complication often associated with effectors is high sequence diversity lack identifiable motifs precluding prediction structure function. In recent years, several...
Plant pathogens secrete small proteins, of which some are effectors that promote infection. During colonization the tomato xylem vessels fungus Fusarium oxysporum f.sp. lycopersici (Fol) secretes proteins referred to as SIX (Secreted In Xylem) proteins. Of these, Six1 (Avr3), Six3 (Avr2), Six5, and Six6 required for full virulence, denoting them effectors. To investigate their activities in plant, sap proteome plants inoculated with Fol wild-type or either AVR2, AVR3, SIX2, SIX5, SIX6...
Molecular interaction between the causal agent of blackleg disease, Leptosphaeria maculans (Lm), and its host, Brassica napus, is largely unknown. We applied a deep RNA-sequencing approach to gain insight into pathogenicity mechanisms Lm defence response B. napus. RNA from infected susceptible napus cultivar Topas DH16516, sampled at 2-day intervals (0-8 days), was sequenced used for gene expression profiling. Patterns regulation in showed multifaceted responses evident by differential genes...
Abstract Employing race‐specific resistance genes remains an effective strategy to protect wheat from leaf rust caused by Puccinia triticina (Pt) worldwide, while the newly emerged Pt races, owing rapid genetic evolution, frequently overcome immune response delivered genes. The molecular mechanisms underlying evolved virulence pathogen remain unknown. Here, we identified avirulence protein AvrLr15 that induced Lr15 ‐dependent responses. Heterologously produced triggered pronounced cell death...
Plant pathogens secrete effector proteins to promote host colonization. During infection of tomato xylem vessels, Fusarium oxysporum f. sp. lycopersici (Fol) secretes the Avr2 protein. Besides being a virulence factor, is recognized intracellularly by I-2 resistance protein, resulting in induction defenses. Here, we show that AVR2 highly expressed root- and xylem-colonizing hyphae three days post inoculation roots. Co-expression with deletion constructs using agroinfiltration Nicotiana...
The fungus Leptosphaeria maculans (L. maculans) is the causal agent of blackleg disease canola/oilseed rape (Brassica napus) worldwide. We previously reported cloning B. napus resistance gene, LepR3, which encodes a receptor-like protein. LepR3 triggers localized cell death upon recognition its cognate Avr protein, AvrLm1. Here, we exploited Nicotiana benthamiana model plant to investigate mechanism AvrLm1 by LepR3. Co-expression LepR3/AvrLm1 gene pair in N. resulted development...
Abstract Plant NDR1/HIN1-like ( NHL ) genes play an important role in triggering plant defenses response to biotic stresses. In this study, we performed a genome-wide identification of the pepper Capsicum annuum L.) and characterized functional roles these CaNHL abiotic stresses infection by different pathogens. Phylogenetic analysis revealed that s can be classified into five distinct subgroups, with each group containing generic specific motifs. Regulatory element showed majority promoter...
Structures of nitrogenases, dark-operative protochlorophyllide oxidoreductases, and light-dependent oxidoreductases (LPOR) have been resolved. However, their evolutionary relatedness remains elusive. Here, we show, through structural alignment, that all subunits nitrogenase-like proteins originated from a co-ancestral archaic one-subdomain precursor. LPOR evolved the BchX/BchY chlorophyllide oxidoreductase (COR), intermediary retinol dehydrogenase possible genetic recombination. We thus...
Leptosphaeria maculans, the causal agent of blackleg disease in canola (Brassica napus), secretes an array effectors into host to overcome defense. Here we present evidence that L. maculans effector protein AvrLm1 functions as a virulence factor by interacting with B. napus mitogen-activated (MAP) kinase 9 (BnMPK9), resulting increased accumulation and enhanced phosphorylation protein. Transient expression BnMPK9 Nicotiana benthamiana induces cell death, this phenotype is presence AvrLm1,...
Abstract BACKGROUND Development of anti‐plant‐virus compounds and improvement biosafety remain hot research topics in controlling plant viral disease. Tobacco mosaic virus (TMV) infects all tobacco species as well many other plants worldwide causes severe losses production. To date, no efficient chemical treatments are known to protect from infection. Therefore, the search for a highly active antiviral compound with high efficacy field application is required. RESULTS We reported synthesis...
Sclerotinia sclerotiorum is a characteristic necrotrophic plant pathogen and dependent on the induction of host cell death for nutrient acquisition. To identify necrosis-inducing effectors, genome S. was scanned genes encoding small, secreted, cysteine-rich proteins. These potential effectors were tested their ability to induce necrosis in Nicotiana benthamiana via Agrobacterium-mediated expression cellular localization cells. Six novel proteins discovered, which all but one required signal...
Fusarium oxysporum f. sp. medicaginis (Fom) and Rhizoctonia solani (Rs) are the major soil-borne fungal pathogens that pose severe threats to commercial alfalfa production in China. However, effects of Fom Rs co-infection on whether alters disease resistance responses among diverse varieties remain unknown. A collection 80 ( Medicago sativa ) originated from seven countries were used study host responses. The resulted more reductions growth biomass allocation across comparison with either...
Fusarium oxysporum f. sp. lycopersici (Fol) causes vascular wilt disease in tomato. Upon colonization of the host, Fol secretes many small effector proteins into xylem sap to facilitate infection. Besides known SIX (secreted xylem) proteins, identity additional effectors that contribute pathogenicity remains largely unexplored. We performed a deep RNA-sequencing analysis race 2-infected tomato, used sequence data annotate published genome assembly generated via PacBio SMRT sequencing 2...
Puccinia triticina (Pt), as the causal agent of wheat leaf rust, employs a plethora effector proteins to modulate immunity for successful colonization. Understanding molecular mechanisms underlying Pt effector-mediated susceptibility remains largely unexplored. In this study, an Pt_21 was identified interact with apoplast-localized thaumatin-like protein TaTLP1 using yeast two-hybrid assay and Pt_21-TaTLP1 interaction characterized. The between validated by in vivo co-immunoprecipitation...
Prevention of autonomous division the egg apparatus and central cell in a female gametophyte before fertilization ensures successful reproduction flowering plants. Here we show that rice ovules Polycomb repressive complex 2 (PRC2) Osfie1 Osfie2 double mutants exhibit asexual embryo endosperm formation at high frequency, while single display pre-embryo-like structures lower frequency without fertilization. Earlier onset, higher penetrance better development embryos compared with those suggest...