A5020457527- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Plant Pathogenic Bacteria Studies
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Autophagy in Disease and Therapy
- Plant responses to water stress
- Cassava research and cyanide
- Plant nutrient uptake and metabolism
- Light effects on plants
- Plant Pathogens and Resistance
- Enzyme-mediated dye degradation
- Plant Stress Responses and Tolerance
- Forest Insect Ecology and Management
University of Warwick
2015-2025
Hebei Agricultural University
2023-2025
Hangzhou Normal University
2015-2024
University of Worcester
2017-2024
National Cheng Kung University Hospital
2021
Coventry (United Kingdom)
2013
Tsinghua University
2010-2012
South China Sea Institute Of Oceanology
2010
Warwick Hospital
2009
University of Leeds
2008
Ethylene is required for climacteric fruit ripening. Inhibition of ethylene biosynthesis genes, 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase, prevents or delays ripening, but it not known how these genes are modulated during normal development. LeHB-1, a previously uncharacterized tomato homeobox protein, was shown by gel retardation assay to interact with the promoter LeACO1, an oxidase gene expressed LeHB-1 mRNA accumulation in fruit, using virus-induced silencing,...
Significance This work shows that active DNA demethylation governs ripening, an important plant developmental process. Our defines a molecular mechanism, which has until now been missing, to explain the correlation between genomic and fruit ripening. It demonstrates direct cause-and-effect relationship induction of gene expression in fruits. The importance these findings goes far beyond understanding biology ripening provides innovative strategy for its fine control through modulation...
Abstract Transitory starch, a major photosynthetic product in the leaves of land plants, accumulates chloroplasts during day and is hydrolyzed to maltose Glc at night support respiration metabolism. Previous studies Arabidopsis thaliana indicated that degradation transitory starch only occurs chloroplasts. Here, we report autophagy, nonplastidial process, participates leaf degradation. Excessive accumulation was observed Nicotiana benthamiana seedlings treated with an autophagy inhibitor...
Autophagy is an evolutionarily conserved process that recycles damaged or unwanted cellular components, and has been linked to plant immunity. However, how autophagy contributes immunity unknown. Here we reported the autophagic machinery targets virulence factor βC1 of Cotton leaf curl Multan virus (CLCuMuV) for degradation through its interaction with key protein ATG8. A V32A mutation in abolished NbATG8f, carrying βC1V32A showed increased symptoms viral DNA accumulation plants....
Autophagy as a conserved catabolic pathway can respond to reactive oxygen species (ROS) and plays an important role in degrading oxidized proteins plants under various stress conditions. However, how ROS regulates autophagy response oxidative stresses is largely unknown. Here, we show that autophagy-related protein 3 (ATG3) interacts with the cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs) regulate Nicotiana benthamiana plants. We found inhibits interaction of ATG3 GAPCs....
Kiwifruit (Actinidia chinensis) is one of the popular fruits world-wide, and its quality mainly determined by key metabolites (sugars, flavonoids, vitamins). Previous works on kiwifruit are mostly done via a single omics approach or involve only limited metabolites. Consequently, dynamic metabolomes during development ripening underlying regulatory mechanisms poorly understood. In this study, using high-resolution metabolomic transcriptomic analyses, we investigated metabolic landscapes at...
The complete nucleotide sequence of the DNA Indian cassava mosaic virus (ICMV) and a key part that group B isolate African from Malawi (ACMV-M) were determined compared at encoded amino acid levels with published sequences an ACMV A (ACMV-K) other whitefly-transmitted geminiviruses (WTGs). ICMV consists two circular single-stranded molecules, DNA-A [2815 nucleotides (nt)] DNA-B (2645 nt), which differ substantially in genome components ACMV-K (DNA-A 70%, 47% identity) WTGs. contains eight...
Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA technique for plant functional genomics. Besides RNAs, microRNAs (miRNAs) have also been shown to regulate expression by in various organisms. However, miRNA has not reported. In addition, number of miRNAs identified or predicted, while their functions are largely unknown. Thus, there an urgent need the development new technologies study function. Here, we report that modified cabbage...
Gene silencing is a natural antiviral defense mechanism in plants. For effective infection, plant viruses encode viral suppressors to counter this response. The geminivirus-encoded C4 protein has been identified as gene suppressor, but the underlying of action not characterized. Here, we report that Cotton Leaf Curl Multan virus (CLCuMuV) interacts with S-adenosyl methionine synthetase (SAMS), core enzyme methyl cycle, and inhibits SAMS enzymatic activity. By contrast, an R13A mutation...
Viruses interfere with and usurp host machinery circumvent defense responses to create a suitable cellular environment for successful infection. This is usually achieved through interactions between viral proteins factors. Geminiviruses are group of plant-infecting DNA viruses, which some contain betasatellite, known as DNAβ. Here, we report that Cotton leaf curl Multan virus (CLCuMuV) uses its sole satellite-encoded protein βC1 regulate the plant ubiquitination pathway effective We found...
Virus-induced gene silencing (VIGS) is a powerful technique to study function in plants. However, very few VIGS vectors are available for monocot Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective system induce efficient of endogenous genes plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail millet (Setaria italica). This evidenced by FoMV-based phytoene desaturase (PDS) magnesium chelatase barley, PDS Cloroplastos alterados1...
Abstract Autophagy plays an important role in plant–pathogen interactions. Several pathogens including viruses induce autophagy plants, but the underpinning mechanism remains largely unclear. Furthermore, virus–plant interactions, viral factor(s) that have yet to be identified. Here, we report βC1 protein of Cotton leaf curl Multan betasatellite (CLCuMuB) interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), a negative autophagic regulator, Nicotiana benthamiana. CLCuMuB...
Summary The plant hormone ethylene plays a critical role in fruit defense against Botrytis cinerea attack, but the underlying mechanisms remain poorly understood. Here, we showed that response factor SlERF.C1 acts as key regulator to trigger ethylene‐mediated B. tomato fruits without compromising ripening. Knockout of increased susceptibility with no effect on ripening process, while overexpression enhanced resistance. RNA‐Seq, transactivation assays, EMSA and ChIP‐qPCR results indicated...
The nuclear localized C2 protein of the monopartite bego-movirus Tomato yellow leaf curl virus-China (TYLCV-C) contributes to viral pathogenicity. Here, we have investigated TYLCV-C domains that play a role in phenotype. Alignment with 67 homologues from and bipartite begomoviruses re-vealed putative zinc-finger motif C 36 -X1-C 38 -X7-C 46 -X6-H 53 -X4-H 58 59 four potential phosphorylation sites(T 52 , S 61 Y 68 74 ) are highly conserved. When ex-pressed Potato virus X (PVX) vector,...
ABSTRACT Virulence-attenuating hypoviruses of the species Cryphonectria hypovirus 1 (CHV1) encode a papain-like protease, p29, that shares similarities with potyvirus-encoded suppressor RNA silencing HC-Pro. We now report CHV1-EP713-encoded p29 can suppress in natural host, chestnut blight fungus parasitica . Hairpin RNA-triggered was suppressed C. strains expressing and transformation transgenic green fluorescent protein (GFP)-silenced strain resulted an increased number transformants...
The nucleus-localized C2 protein of Tomato yellow leaf curl virus-China (TYLCV-C) is an active suppressor posttranscriptional gene silencing (PTGS). Consistently, infection with TYLCV-C resulted in PTGS arrest plants. possesses a functional, arginine-rich nuclear localization signal within the basic amino acid-rich region (17)KVQHRIAKKTTRRRR(31). When expressed from potato virus X, C2-RRRR(31)DVGG (in which four consecutive arginine residues (28)RRRR(31) were replaced DVGG) that had been...