A5039087610- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Autophagy in Disease and Therapy
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Cancer-related molecular mechanisms research
- Aquaculture disease management and microbiota
- Plant responses to water stress
- Light effects on plants
- Toxin Mechanisms and Immunotoxins
- Plant and Fungal Interactions Research
- Plant Pathogens and Resistance
- CRISPR and Genetic Engineering
- Plant and animal studies
- Protein Hydrolysis and Bioactive Peptides
- Plant Disease Management Techniques
- Corneal Surgery and Treatments
- Advanced Biosensing Techniques and Applications
- Insect Resistance and Genetics
Shanghai East Hospital
2024
Hebei Medical University
2024
Shanghai Cell Therapy Research Institute
2024
Texas A&M University System
2019-2023
Texas A&M University
2018-2022
Mitchell Institute
2022
ZheJiang Academy of Agricultural Sciences
2015-2021
State Key Laboratory of Chemobiosensing and Chemometrics
2021
Hunan Agricultural University
2021
Research Institute of Dallas
2021
Abstract Transitory starch, a major photosynthetic product in the leaves of land plants, accumulates chloroplasts during day and is hydrolyzed to maltose Glc at night support respiration metabolism. Previous studies Arabidopsis thaliana indicated that degradation transitory starch only occurs chloroplasts. Here, we report autophagy, nonplastidial process, participates leaf degradation. Excessive accumulation was observed Nicotiana benthamiana seedlings treated with an autophagy inhibitor...
Autophagy as a conserved catabolic pathway can respond to reactive oxygen species (ROS) and plays an important role in degrading oxidized proteins plants under various stress conditions. However, how ROS regulates autophagy response oxidative stresses is largely unknown. Here, we show that autophagy-related protein 3 (ATG3) interacts with the cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs) regulate Nicotiana benthamiana plants. We found inhibits interaction of ATG3 GAPCs....
Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA technique for plant functional genomics. Besides RNAs, microRNAs (miRNAs) have also been shown to regulate expression by in various organisms. However, miRNA has not reported. In addition, number of miRNAs identified or predicted, while their functions are largely unknown. Thus, there an urgent need the development new technologies study function. Here, we report that modified cabbage...
Viruses interfere with and usurp host machinery circumvent defense responses to create a suitable cellular environment for successful infection. This is usually achieved through interactions between viral proteins factors. Geminiviruses are group of plant-infecting DNA viruses, which some contain betasatellite, known as DNAβ. Here, we report that Cotton leaf curl Multan virus (CLCuMuV) uses its sole satellite-encoded protein βC1 regulate the plant ubiquitination pathway effective We found...
Virus-induced gene silencing (VIGS) is a powerful technique to study function in plants. However, very few VIGS vectors are available for monocot Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective system induce efficient of endogenous genes plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail millet (Setaria italica). This evidenced by FoMV-based phytoene desaturase (PDS) magnesium chelatase barley, PDS Cloroplastos alterados1...
Clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) based genome editing technology has been developed from the adaptive immune system used by many bacteria and archaea for combating against viruses. Here, we utilize Cas9 with dual gRNAs designed to target two essential regions of single-stranded DNA Cotton Leaf Curl Multan virus (CLCuMuV) which can cleavage corresponding sequence thus interrupt viral infection. The transgenic plants expressing...
The multifunctional movement protein (MP) of Tomato mosaic tobamovirus (ToMV) is involved in viral cell-to-cell movement, symptom development, and resistance gene recognition. However, it remains to be elucidated how ToMV MP plays such diverse roles plants. Here, we show that interacts with the Rubisco small subunit (RbCS) Nicotiana benthamiana vitro vivo. In susceptible N. plants, silencing NbRbCS enabled induce necrosis inoculated leaves, thus enhancing virus local infectivity. development...
Objective: Diagnosis of schizophrenia is currently dependent on symptom-based criteria and lacks objective indicators. In this study, the authors investigated whether circulating miRNA can serve as a diagnostic biomarker for schizophrenia. Methods: Global plasma miRNAs were profiled in test cohort 164 patients 187 control subjects, using Solexa sequencing, TaqMan Low Density Array, quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. The captured then validated by...
MicroRNAs (miRNAs) play pivotal roles in various biological processes across kingdoms. Many plant miRNAs have been experimentally identified or predicted by bioinformatics mining of small RNA databases. However, the functions these remain largely unknown due to lack effective genetic tools. Here, we report a virus-based microRNA silencing (VbMS) system that can be used for functional analysis miRNAs. VbMS is performed through tobacco rattle expression miRNA target mimics silence endogenous...
Viral infections cause plant chlorosis, stunting, necrosis or other symptoms. The down-regulation of chloroplast-related genes (ChRGs) is assumed to be responsible for chlorosis. We identified the differentially expressed (DEGs) in Rice stripe virus (RSV)-infected Nicotiana benthamiana, and examined contribution 75 down-regulated DEGs RSV symptoms by silencing them one using Tobacco rattle (TRV)-induced gene silencing. Silencing 11 caused nine were ChRGs. a DEG encoding eukaryotic...
Summary The hypersensitive‐induced reaction ( HIR ) gene family is associated with the hypersensitive response HR that a part of plant defense system against bacterial and fungal pathogens. involvement genes in to viral pathogens has not yet been studied. We now report 3 Nicotiana benthamiana Oryza sativa (rice) were upregulated following rice stripe virus RSV infection. Silencing s N. resulted an increased accumulation RNA s, whereas overexpression 3s or reduced expression decreased symptom...
The tomato Tobacco mosaic virus resistance-22 (Tm-22) gene encodes a coiled-coil-nucleotide binding site-Leu-rich repeat protein lacking conventional plasma membrane (PM) localization motif. Tm-22 confers plant extreme resistance against tobamoviruses including (TMV) by recognizing the avirulence (Avr) viral movement (MP). However, subcellular compartment where functions is unclear. Here, we demonstrate that interacts with TMV MP to form complex at PM. We show both inactive and active...
Tm-22 is a coiled coil-nucleotide binding-leucine rich repeat resistance protein that confers durable extreme against Tomato mosaic virus (ToMV) and Tobacco (TMV) by recognizing the viral movement (MP). Here we report Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, SGT1. Silencing of NbMIP1s reduced TMV compromised Tm-22-mediated ToMV. Furthermore, silencing steady-state levels ToMV MP Tm-22. Moreover, are required for plant induced other R genes nonhost...
Jasmonic acid (JA) is a crucial hormone in plant antiviral immunity. Increasing evidence shows that viruses counter this host immune response by interfering with JA biosynthesis and signaling. However, the mechanism which affect still largely unexplored. Here, we show highly conserved chloroplast protein cpSRP54 was downregulated Nicotiana benthamiana infected turnip mosaic virus (TuMV). Its silencing facilitated TuMV infection. Furthermore, interacted allene oxide cyclases (AOCs), key...
Plant microRNAs (miRNAs) play pivotal roles in many biological processes. Although miRNAs have been identified various plant species, the functions of these remain largely unknown due to shortage effective genetic tools block their functional activity. Recently, miRNA target mimic (TM) technologies applied perturb activity specific endogenous or families. We previously reported that Tobacco rattle virus (TRV)-based TM expression can successfully mediate virus-based silencing/suppression...
RNA silencing is an evolutionarily conserved antiviral mechanism, through which virus-derived small interfering RNAs (vsiRNAs) playing roles in host defence are produced virus-infected plant. Deep sequencing technology has revolutionized the study on interaction between virus and plant analysis of vsiRNAs profile. However, comparison vsiRNA profiles different tissues from a same been rarely reported. In this study, leaves fruits Lagenaria siceraria plants infected with Cucumber green mottle...
Rice stripe virus (RSV) is one of the most devastating viral pathogens in rice and can also cause general chlorosis symptom
Leptospirosis is a re-emerging tropical infectious disease caused by pathogenic Leptospira spp. The different host innate immune responses are partially related to the severities of leptospirosis. In this study, we employed transcriptomics and cytokine arrays comparatively calculate murine peritoneal macrophages (MPMs) human peripheral blood monocytes (HBMs) leptospiral infection. We uncovered series expression profiles these two cells. percentages regulated genes in several biological...
Garlic virus X (GarVX) ORF3 encodes a p11 protein, which contributes to cell-to-cell movement and forms granules on the endoplasmic reticulum (ER) in Nicotiana benthamiana. Expression of either from binary vector, PVX or TMV induced ER stress unfolded protein response (UPR), as demonstrated by an increase transcription luminal binding (BiP) bZIP60 genes. UPR-related programmed cell death (PCD) was elicited : systemic infected leaves. Examination mutants with deletions two transmembrane...
AGD2-LIKE DEFENCE RESPONSE PROTEIN 1 (ALD1) triggers plant defence against bacterial and fungal pathogens by regulating the salicylic acid (SA) pathway an unknown SA-independent pathway. We now show that Nicotiana benthamiana ALD1 is involved in a virus ethylene also participates ALD1-mediated resistance. NbALD1 was up-regulated plants infected with turnip mosaic (TuMV). Silencing of facilitated TuMV infection, while overexpression or exogenous application pipecolic (Pip), downstream product...