A5003946127- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Aquaculture disease management and microbiota
- Neuroinflammation and Neurodegeneration Mechanisms
- Pulmonary Hypertension Research and Treatments
- RNA modifications and cancer
- Retinal Diseases and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Immune cells in cancer
- Identification and Quantification in Food
- Uterine Myomas and Treatments
- Nitric Oxide and Endothelin Effects
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Adipose Tissue and Metabolism
- Advanced Chemical Sensor Technologies
- Biochemical and Molecular Research
- Invertebrate Immune Response Mechanisms
- ATP Synthase and ATPases Research
- Electrocatalysts for Energy Conversion
- Chronic Kidney Disease and Diabetes
- Endometriosis Research and Treatment
- Epigenetics and DNA Methylation
- Atherosclerosis and Cardiovascular Diseases
Augusta University
2015-2024
Fujian Fisheries Research Institute
2015-2024
Augusta University Health
2016-2024
Fujian Agriculture and Forestry University
2024
Yuhuangding Hospital
2017-2023
Affiliated Hospital of Qingdao University
2017-2023
Qingdao University
2017-2023
Southern University of Science and Technology
2023
Tianjin University
2022
Peking University
2017-2020
Significance Lung endothelial cells express high levels of glucose metabolic enzymes, such as PFKFB3, and consequently produce large amounts metabolites. These metabolites are able to stabilize the cell signaling molecule HIF2A, similar that which occurs under hypoxic conditions. This stabilization HIF2A by in lung stimulates production growth inflammatory factors, thereby enhancing proliferation inflammation pulmonary vessels exacerbating hypertension (PH). In this study, blockade PFKFB3...
Abstract Increased aerobic glycolysis in endothelial cells of atheroprone areas blood vessels has been hypothesized to drive increased inflammation and lesion burden but direct links remain be established. Here we show that exposed disturbed flow vivo vitro exhibit levels protein kinase AMP-activated (PRKA)/AMP-activated kinases (AMPKs). Selective deletion Prkaa1 , coding for catalytic subunit alpha1, reduces glycolysis, compromises cell proliferation, accelerates the formation...
Adenosine/adenosine receptor-mediated signaling has been implicated in the development of various ischemic diseases, including retinopathies. Here, we show that adenosine A2a receptor (ADORA2A) promotes hypoxia-inducible transcription factor-1 (HIF-1)-dependent endothelial cell glycolysis, which is crucial for pathological angiogenesis proliferative Adora2a expression markedly increased retina mice with oxygen-induced retinopathy (OIR). Endothelial cell-specific, but not macrophage-specific...
Increased glycolysis facilitates reciprocal activation between myeloid cells and endothelial in pathological retinal angiogenesis.
The molecular mechanisms underlying vascular inflammation and associated inflammatory diseases are not well defined. Here we show that endothelial intracellular adenosine its key regulator kinase (ADK) play important roles in inflammation. Pro-inflammatory stimuli lead to by increasing ADK expression, reducing the level of cells, activating transmethylation pathway through association with S-adenosylhomocysteine (SAH) hydrolase (SAHH). Increasing genetic knockdown or exogenous reduces...
Abstract The nucleoside adenosine is a potent regulator of vascular homeostasis, but it remains unclear how expression or function the adenosine‐metabolizing enzyme kinase (ADK) and intracellular levels influence angiogenesis. We show here that hypoxia lowered ADK increased in human endothelial cells. Knockdown (KD) elevated adenosine, promoted proliferation, migration, angiogenic sprouting Additionally, mice deficient displayed angiogenesis as evidenced by rapid development retinal...
Adenosine 2A receptor (A2AR) exerts protective roles in endotoxin‐ and/or ischemia‐induced tissue damage. However, the role for A2AR nonalcoholic fatty liver disease (NAFLD) remains largely unknown. We sought to examine effects of global myeloid cell‐specific disruption on aspects obesity‐associated NAFLD and elucidate underlying mechanisms. Global cell–specific A2AR‐disrupted mice control were fed a high‐fat diet (HFD) induce NAFLD. In addition, bone marrow–derived macrophages primary mouse...
Spontaneous intracerebral hemorrhage (ICH) produces the highest acute mortality and worst outcomes of all stroke subtypes. Hematoma volume is an independent determinant ICH patient outcomes, making clot resolution a primary goal clinical management. Herein, remote-limb ischemic post-conditioning (RIC), repetitive inflation-deflation blood pressure cuff on limb, accelerated hematoma improved neurological after in mice. Parabiosis studies revealed RIC via humoral-mediated mechanism. Whereas...
Pathological angiogenesis is a major cause of irreversible blindness in individuals all age groups with proliferative retinopathy (PR). Mononuclear phagocytes (MPs) within neovascular areas contribute to aberrant retinal angiogenesis. Due their cellular heterogeneity, defining the roles MP subsets PR onset and progression has been challenging. Here, we aimed investigate heterogeneity microglia associated neovascularization characterize transcriptional profiles metabolic pathways...
The eye is closely connected to the brain, providing a unique window detect pathological changes in brain. In this study, we discovered β-amyloid (Aβ) deposits along ocular glymphatic system patients with Alzheimer’s disease (AD) and 5×FAD transgenic mouse model. Interestingly, Aβ from brain can flow into eyes optic nerve through cerebrospinal fluid (CSF), causing retinal degeneration. mainly observed sheath, neural axon, perivascular space, which might represent critical steps of...
Background and Purpose Inactivation of the gene for adenosine A 2A receptors (ADORA2A humans Adora2a rodents) protects against brain injury in experimental stroke. However, cell‐specific pathogenic effects thromboembolic stroke underlying mechanisms remain undefined. Here, we tested hypothesis that inhibition endothelial after improves post‐stroke outcomes via down‐regulation inflammation. Experimental Approach Thromboembolic was induced by embolic middle cerebral artery occlusion mice....
Pathological angiogenesis is a major cause of irreversible blindness in individuals with neovascular age-related macular degeneration (nAMD). Macrophages and microglia (MΦ) contribute to aberrant ocular angiogenesis. However, the role glucose metabolism MΦ nAMD still undefined. Here, we have investigated involvement glycolysis, driven by kinase/phosphatase PFKFB3, development choroidal neovascularization (CNV).CNV was induced mice laser photocoagulation. Choroid/retinal pigment epithelium...
Proliferation of vascular smooth muscle cells (VSMCs) is a hallmark arterial diseases, especially in restenosis after angioplasty or stent placement. VSMCs reprogram their metabolism to meet the increased requirements lipids, proteins, and nucleotides for proliferation. De novo purine synthesis one critical pathways nucleotide synthesis. However, its role proliferation these diseases has not been defined.
Renal fibrosis, a hallmark of chronic kidney diseases, is driven by the activation renal fibroblasts. Recent studies have highlighted role glycolysis in this process. Nevertheless, one critical glycolytic activator, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), remains unexplored fibrosis. Upon reanalyzing single-cell sequencing data from Dr. Humphreys’ lab, we noticed an upregulation glycolysis, gluconeogenesis, and TGFβ signaling pathway myofibroblasts fibrotic kidneys...
Background and Purpose Macrophage infiltration into the lungs is a characteristic of pulmonary hypertension (PH). Glycolysis main metabolic pathway for macrophage activation. However, effect glycolysis on development PH remains unknown. We investigated 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase 3 (PFKBF3), critical enzyme glycolysis, development. Experimental Approach Lung tissues from patients were examined by immunostaining with markers. was induced in Wistar rats SU5416/hypoxia...
Abstract Aims Proliferation of vascular smooth muscle cells (VSMCs) is a hallmark pulmonary hypertension (PH). Proliferative utilize purine bases from the de novo synthesis (DNPS) pathways for nucleotide synthesis; however, it unclear whether DNPS plays critical role in VSMC proliferation during development PH. The last two steps are catalysed by enzyme 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase (ATIC). This study investigated...
Anti–vascular endothelial growth factor therapy has had a substantial impact on the treatment of choroidal neovascularization (CNV) in patients with neovascular age-related macular degeneration (nAMD), leading cause vision loss older adults. Despite treatment, many nAMD still develop severe and irreversible visual impairment because development subretinal fibrosis. We recently reported anti-inflammatory antiangiogenic effects inhibiting gene encoding adenosine receptor 2A ( Adora2a ), which...
Cardiovascular diseases (CVDs) are the leading cause of death worldwide, and morbidity mortality rates continue to rise. Atherosclerosis constitutes principal etiology CVDs. Endothelial injury, inflammation, dysfunction initiating factors atherosclerosis. Recently, we reported that endothelial adenosine receptor 2 A (ADORA2A), a G protein-coupled (GPCR), plays critical roles in neovascularization disease cerebrovascular disease. However, precise role ADORA2A atherosclerosis is still not...
Abstract Aims Abdominal aortic aneurysm (AAA) is a common, serious vascular disease with no effective pharmacological treatment. The nucleoside adenosine plays an important role in modulating homeostasis, which prompted us to determine whether kinase (ADK), metabolizing enzyme, modulates AAA formation via control of the intracellular level, and investigate underlying mechanisms. Methods results We used combination genetic approaches murine models induced by calcium chloride (CaCl2)...
Adenosine receptors and extracellular adenosine have been demonstrated to modulate vascular smooth muscle cell (VSMC) proliferation neointima formation. kinase (ADK) is a major enzyme regulating intracellular levels but function in VSMC remains unclear. Here, we investigated the role of ADK injury-induced delineated mechanisms underlying its action.
Exploring effective synthetic protocols for electrocatalysts of the oxygen reduction reaction (ORR) is vital to practical application alkaline fuel cells. Herein, a high-temperature shock (HTS) method used synthesize Pt–Ni alloy nanoparticles with various Pt/Ni ratios on carbon support. The provided by Joule effect current passing through paper. ultrafast quenching process after second-long endows as-synthesized an adjustable size and kinetically trapped dislocation features, which provides...