A5044690473- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Adenosine and Purinergic Signaling
- Ubiquitin and proteasome pathways
- Cancer, Lipids, and Metabolism
- Gut microbiota and health
- Prostate Cancer Treatment and Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
- Gastrointestinal motility and disorders
- Pelvic floor disorders treatments
- Chemokine receptors and signaling
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Air Quality and Health Impacts
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
Jining Medical University
2019-2025
Shanghai Jiao Tong University
2019-2024
Renji Hospital
2021-2024
Shanghai Cancer Institute
2024
State Key Laboratory of Oncogene and Related Genes
2021
Shanghai Botanical Garden
2020
Hunan Normal University
2019-2020
XinHua Hospital
2014-2020
Hunan Provincial People's Hospital
2019-2020
Institute of Microbiology
2015-2020
Recently, increasing evidence has suggested the association between gut dysbiosis and Alzheimer's disease (AD) progression, yet role of microbiota in AD pathogenesis remains obscure. Herein, we provide a potential mechanistic link neuroinflammation progression. Using mouse models, discovered that, during alteration composition leads to peripheral accumulation phenylalanine isoleucine, which stimulates differentiation proliferation pro-inflammatory T helper 1 (Th1) cells. The...
•Epithelial subtype 1, 2 and 3 were mainly found in the adenocarcinoma 4 was present adenosquamous carcinoma.•Tumors with ErbB pathway mutations harbored a larger population of 1 epithelial cells, Tregs, M2 macrophages.•Increased MDK these tumors interacted its receptor LRP1 to promote immunosuppressive macrophage differentiation.•Crosstalk between macrophage-secreted CXCL10 CXCR3 on Tregs induced GBC mutations. Background & AimsOur previous genomic whole-exome sequencing (WES) data...
Adenosine/adenosine receptor-mediated signaling has been implicated in the development of various ischemic diseases, including retinopathies. Here, we show that adenosine A2a receptor (ADORA2A) promotes hypoxia-inducible transcription factor-1 (HIF-1)-dependent endothelial cell glycolysis, which is crucial for pathological angiogenesis proliferative Adora2a expression markedly increased retina mice with oxygen-induced retinopathy (OIR). Endothelial cell-specific, but not macrophage-specific...
The molecular mechanisms underlying vascular inflammation and associated inflammatory diseases are not well defined. Here we show that endothelial intracellular adenosine its key regulator kinase (ADK) play important roles in inflammation. Pro-inflammatory stimuli lead to by increasing ADK expression, reducing the level of cells, activating transmethylation pathway through association with S-adenosylhomocysteine (SAH) hydrolase (SAHH). Increasing genetic knockdown or exogenous reduces...
Abstract The nucleoside adenosine is a potent regulator of vascular homeostasis, but it remains unclear how expression or function the adenosine‐metabolizing enzyme kinase (ADK) and intracellular levels influence angiogenesis. We show here that hypoxia lowered ADK increased in human endothelial cells. Knockdown (KD) elevated adenosine, promoted proliferation, migration, angiogenic sprouting Additionally, mice deficient displayed angiogenesis as evidenced by rapid development retinal...
Growing evidence has shown that the gut-renal connection and gut microbiota dysbiosis play a critical role in immunoglobulin A nephropathy (IgAN). However, fecal microbiome profile Chinese patients with IgAN remains unknown. cross-sectional study was designed for first time to investigate compositions primary China evaluate relationship between clinical presentation.Fecal samples were collected from 17 18 age-, sex-, body mass index-matched healthy controls, bacterial DNA extracted 16S...
Abstract Rasfonin is a fungal secondary metabolite with demonstrated antitumor effects. However, the underlying mechanism of regulatory role in autophagy initiated by rasfonin largely unknown. Moreover, function Akt to positively mediate induced remains elusive. In present study, we observed that concomitant upregulation phosphorylation. Both inhibition small molecule inhibitors and genetic modification partially reduced rasfonin-dependent autophagic flux PARP-1 cleavage. The overexpression...
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3), a glycolytic enzyme highly expressed in cancer cells, has been reported to participate regulating metabolism, angiogenesis, and autophagy. Although anti-cancer drug oxaliplatin (Oxa) effectively inhibits cell proliferation induces apoptosis, the growing resistance side-effects make it urgent improve therapeutic strategy of Oxa. Oxa autophagy process, role PFKFB3 this process remains unknown. In addition, whether affects...
Soy isoflavones are natural tyrosine kinase inhibitors closely associated with decreased morbidity and mortality of various tumors. The activation kinases such as ERBB2 is the mechanism by which cholecystitis transforms into gallbladder cancer (GBC), therefore, it important to investigate relationship between long-term exposure soy occurrence progression GBC. This case-control study (n = 85 pairs) found that high level plasma isoflavone-genistein (GEN) was a lower risk (≥326.00 ng/mL...
Rationale: Considerable evidence suggests that breast cancer metastasis and recurrence occur due to emergence of stem cells (CSCs). In our previous study, we designed a high-throughput siRNA screening platform identifies inflammation genes involved in the regulation cell stemness. We reported CCL16 protein decreases OCT4 expression reduces ALDH+ subpopulation. However, mechanism by which maintains cell-like properties remains unclear. Methods: Tissue microarrays were used evaluate...
Anthrax toxin receptor 1 (ANTXR1), a type I transmembrane protein, is one of the receptors that facilitates entrance anthrax into cells. Previous studies have confirmed pivotal role ANTXR1 in progression and tumorigenesis diverse cancer types. However, biological function gastric (GC) still unknown. The present study aimed to investigate GC illuminate potential molecular mechanisms. Bioinformatics analysis found expression was significantly upregulated tissue its overexpression associated...
Alternol is a naturally occurring compound that exerts antitumor activity in several cancers. However, whether induces immune response remains unknown. In this study, we investigated induced immunogenic cell death (ICD) prostate cancer cells. triggered ICD cells, as evidenced by the release of damage-associated molecular patterns (DAMPs) (i.e., calreticulin, CALR; high mobility group protein B1, HMGB1; and adenosine triphosphate, ATP) pro-inflammatory cytokine interleukin [IL]-1α, IL-1β,...
BACKGROUND As a member of the chaperonin-containing tailless complex polypeptide 1 (TCP1) complex, which plays pivotal role in ensuring accurate folding numerous proteins, TCP1 subunit 6A (CCT6A) participates various physiological and pathological processes. However, its effects on cell death cancer therapy underlying mechanisms need further exploration colorectal (CRC) cells. AIM To explore CCT6A CRC. METHODS Cell proliferation was evaluated using MTS assay, EdU staining, colony growth...
// Siyuan Yan 1, 3 , Xiaoli Wei 2 Shanshan Xu Hui Sun Weijun Wang Ling Liu 1 Xuejun Jiang Yongxiang Zhang and Yongsheng Che State Key Laboratory of Mycology, Institute Microbiology, Chinese Academy Sciences, Beijing 100101, China Toxicology & Medical Countermeasures, Pharmacology Toxicology, 100850, University 100039, Correspondence to: Che, email: cheys@im.ac.cn Zhang, zhangyx@bmi.ac.cn Keywords: PFKFB3, glycolysis, nuclear localization, autophagy, AMPK Received: June 06,...
Adenosine receptors and extracellular adenosine have been demonstrated to modulate vascular smooth muscle cell (VSMC) proliferation neointima formation. kinase (ADK) is a major enzyme regulating intracellular levels but function in VSMC remains unclear. Here, we investigated the role of ADK injury-induced delineated mechanisms underlying its action.
Deficiency of autophagy has been linked to increase in nuclear instability, but the role regulating formation and elimination micronuclei, a diagnostic marker for genomic is limited mammalian cells. Utilizing immunostaining subcellular fractionation, we found that either LC3-II or phosphorylated Ulk1 localized nuclei, immunoprecipitation results showed both LC3 Unc-51-like kinase 1 (Ulk1) interacted with γ-H2AX, DNA double-strand breaks (DSB). Sunitinib, multi-targeted receptor tyrosine...
Due to the high-level of metastatic and relapsed rates, rhabdomyosarcoma (RD) patients have a poor prognosis, novel treatment strategies are required. Thereby, present study evaluated efficacy PFK15, PFKFB3 inhibitor, in RD cells explore its potential underlying mechanism on regulation autophagy proliferation these cells. The effects PFK15 cell viability loss death different groups, were by MTS assay, colony growth assay immunoblotting, respectively. In addition, levels detected electron...
Abstract Background The progression of gallbladder cancer (GBC) is accompanied by abnormal fatty acid β-oxidation (FAO) metabolism. Different types lipids perform various biological functions. This study aimed to determine the role acyl carnitines in molecular mechanisms GBC progression. Methods Distribution was described LC–MS-based lipidomics. Cellular localization, expression level and full-length lncBCL2L11 were detected using fluorescence situ hybridization (FISH) assays, subcellular...
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe2O3 nanoparticles. Hepatocarcinoma cultured in vitro were treated ferrofluid containing nanoparticles and irradiated an alternating radio frequency field. The influence treatment on was examined by inverted microscopy, MTT flow cytometry. To MFH, Hsp70, Bax, Bcl-2 p53 detected immunocytochemistry reverse transcription polymerase chain reaction...
Atmospheric particulate matter with a diameter <2.5 µm (PM2.5) and pollution are worldwide environmental problems may have negative effects on cardiovascular disease through the lung gut. The dynamics of intestinal microflora in response to pollutants is unclear. present study investigated changes gut microbiota related pollutant exposure using spontaneously hypertensive rats (SHR). DNA was extracted from fecal samples. Amplicon Generation quality control PCR products were performed....