A5100677494- Signaling Pathways in Disease
- Metabolism and Genetic Disorders
- Renal and related cancers
- Peptidase Inhibition and Analysis
- Mitochondrial Function and Pathology
- Gut microbiota and health
- MicroRNA in disease regulation
- Antibiotics Pharmacokinetics and Efficacy
- Biochemical and Molecular Research
- Plant Pathogens and Fungal Diseases
- RNA Research and Splicing
- DNA Repair Mechanisms
- Genomics, phytochemicals, and oxidative stress
- RNA modifications and cancer
- Heart Rate Variability and Autonomic Control
- Stress Responses and Cortisol
- Cancer-related molecular mechanisms research
- Cardiac electrophysiology and arrhythmias
- Virus-based gene therapy research
- Insect-Plant Interactions and Control
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Mechanisms of cancer metastasis
- Biosensors and Analytical Detection
- Heat shock proteins research
China Medical University
2024
Guangzhou Medical University
2024
Tongji Hospital
2024
Xiamen University
2024
Third Affiliated Hospital of Guangzhou Medical University
2024
Henan Institute of Science and Technology
2020-2024
Institute of Pomology
2024
Hangzhou Dianzi University
2024
First Hospital of Jilin University
2012-2023
Jilin University
2012-2023
The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein endoribonuclease Argonaute and single-stranded small interfering (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- duplex siRNA-initiated RISC activities with use recombinant Drosophila Dicer-2, R2D2, Ago2 proteins. used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter RISC), a Translin Trax. Mg2+-dependent...
Fall armyworm, Spodoptera frugiperda (Smith), has caused significant losses for crop production in China. The fall armyworm is mainly controlled by the chemical insecticides, whereas frequent application of insecticides would result resistance development. Insect cytochrome P450 monooxygenases play an essential part detoxification insecticides. In this study, five genes were selected to determine role response RNA interference (RNAi). Developmental expression pattern analysis revealed that...
Abstract Hepatocellular carcinoma (HCC) is the second leading cause of cancer related-death. As a major common regulator numerous cancer-driving pathways and unique therapeutic target, prolyl isomerase Pin1 overexpressed in majority HCCs, whereas mechanism underlying overexpression remains elusive. Here we find that miR-140-5p inhibits HCC by directly targeting to block multiple pathways. Bioinformatics analysis, miRNA binding functional assays identify interacts with 3′UTR translation....
To determine the oncogenic role of sixth subunit chaperonin-containing tailless complex polypeptide 1 (CCT6A) in hepatocellular carcinoma (HCC) and address correlation CCT6A with clinicopathological characteristics survival. Additionally, this study aimed to explore effect on HCC cells underlying mechanisms. We searched for levels expression Oncomine database GEPIA database, which was then validated by analyzing cancer adjacent non-cancerous tissues patients using quantitative PCR, Western...
Pyroptosis, an inflammatory form of programmed cell death, is the initiating event sepsis and results in immune imbalance by releasing IL‐1 β IL‐18 early stages. Studies show that enhancing autophagy via genetic manipulation can inhibit pyroptosis prolong survival a animal model, indicating possible therapeutic strategy against sepsis. However, almost no study so far has achieved inhibition pharmacological induction disease model. To this end, we established vitro model stimulating primary...
Abstract: Target gene delivery is crucial to therapy. Adeno-associated virus (AAV) has emerged as a primary therapy vector due its broad host range, long-term expression, and low pathogenicity. However, AAV vectors have some limitations, such immunogenicity insufficient targeting. Designing or modifying capsids potential method of improving the efficacy delivery, but hindered by weak biological basis AAV, complexity capsids, limitations current screening methods. Artificial intelligence...
Calcyclin-binding protein (CACYBP) is a multi-ligand implicated in the progression of various human cancers.However, its function hepatocellular carcinoma (HCC) remains unknown.Methods: The expression CACYBP and RNF41 (RING finger 41) HCC cancer adjacent non-tumor tissues was detected by immunohistochemistry. CCK-8 assays, colony formation flow cytometry detection xenograft models were used to evaluate impact on cell growth, apoptosis cycle regulation.Immunoprecipitation ubiquitination...
Quxie capsule(QX), a TCM compound, had shown benefit on survival outcomes for metastatic colorectal cancer(mCRC) patients and could inhibit tumor growth through immune regulation. This study aimed to evaluate whether such effect is associated with gut microbiome modulation.We conducted randomized double-blinded placebo controlled clinical trial in Xiyuan Hospital, China Academy of Chinese Medical Sciences. All were randomly assigned into QX or control group. Before after 1-month...
In antibiotics, β-lactam is one kind of major concern acknowledged as an unavoidable contaminant in milk. Thus, a facile and sensitive method essential for rapid antibiotics detection. our work, specific electrochemical receptor sensor based on the graphene/thionine (GO/TH) composite was established. The mechanism direct competitive inhibition binding horseradish peroxidase-labeled ampicillin (HRP-AMP) to mutant BlaR-CTD protein by free antibiotics. Then, peroxidase (HRP) catalyzed...
Background There is a crosstalk between traditional Chinese medicine (TCM) and gut microbiota (GM), many articles have studied discussed the relationship two. The purpose of this study to use bibliometric analysis explore research status development trends TCM/GM research, identify analyze highly cited papers relating TCM/GM. Methods A literature search regarding publications from 2004 2021 was undertaken on August 13, 2022. main information (full record references) extracted Science...
<title>Abstract</title> Purpose This study aimed to investigate the genetic spectrum of unexplained neonatal seizures in a Chinese population. Methods We retrospectively analyzed 40 neonates with admitted intensive care unit at tertiary hospital between 2016 and 2024. Whole-exome sequencing (WES), clinical exome sequencing, trio-WES, mitochondrial DNA analysis were performed. Results Pathogenic or likely pathogenic variants detected 67.5% (27/40) cases, including, but not limited to,...
Matrix metalloproteinase-9 (MMP-9) plays an important role in inflammation and matrix degradation involved atherosclerosis plaque rupture. The T allele of rs3918242 has been reported to lead a high promoter activity associate with the extent coronary artery disease (CAD). And some studies have that G rs17576 might be associated CAD. aim this study was assess association between polymorphisms MMP-9 gene CAD Chinese Han population.This case-control comprised 258 cases 153 controls from...
The GINS complex is one of the core components eukaryotic replicative helicase CMG (Cdc45-MCM helicase-GINS) that serves as unwinding duplex DNA ahead moving replication fork during chromosome duplication. Many studies have highlighted important functions amongst subunits in various cancers. Nevertheless, and prognostic roles distinct hepatocellular carcinoma (HCC) were largely unexplored. In present study, we reported values HCC patients through analysis several databases, including...
Abstract Aims: A large number of studies have suggested that exportins (XPOs) play a pivotal role in human cancers. In the present study, we analyzed XPO mRNA expression cancer tissues and explored their prognostic value hepatocellular carcinoma (HCC). Methods: Transcriptional survival data related to HCC patients were obtained through ONCOMINE UALCAN databases. Survival analysis plots drawn with Gene Expression Profiling Interactive Analysis (GEPIA). Sequence alteration for XPOs from The...
Atmospheric particulate matter with a diameter <2.5 µm (PM2.5) and pollution are worldwide environmental problems may have negative effects on cardiovascular disease through the lung gut. The dynamics of intestinal microflora in response to pollutants is unclear. present study investigated changes gut microbiota related pollutant exposure using spontaneously hypertensive rats (SHR). DNA was extracted from fecal samples. Amplicon Generation quality control PCR products were performed....