A5020316410- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Entomopathogenic Microorganisms in Pest Control
- Bacterial Genetics and Biotechnology
- Plant Disease Management Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Parasitism and Resistance
- Parasite Biology and Host Interactions
- Insects and Parasite Interactions
- Genetics, Aging, and Longevity in Model Organisms
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- Insect-Plant Interactions and Control
- Food Quality and Safety Studies
- Physiological and biochemical adaptations
- Pediatric health and respiratory diseases
- Insect Resistance and Genetics
- RNA and protein synthesis mechanisms
- Fungal Infections and Studies
- Nitrogen and Sulfur Effects on Brassica
- Fossil Insects in Amber
- Neurobiology and Insect Physiology Research
- Polysaccharides and Plant Cell Walls
- Cellular transport and secretion
- Insect symbiosis and bacterial influences
RIKEN Center for Sustainable Resource Science
2018-2024
Tohoku University
2017
Saga University
2014-2017
Kagoshima University
2016-2017
Kyoto University
2014
Hiroshima University
1981-1984
Institute of Zoology
1983
Plant-parasitic nematodes (PPNs) cause major agricultural losses worldwide, yet the molecular basis of plant immunity against these pathogens remains poorly understood. To investigate how plants recognize PPNs, we aimed to identify microbe-associated patterns (MAMPs) from and corresponding immune components. Due limited availability material obligate used Caenorhabditis elegans , a free-living nematode, as MAMP source. C. extracts activated MAMP-triggered responses in Arabidopsis Col-0....
Root-knot nematodes ( Meloidogyne spp.) cause serious damage to many crops globally. We report the high-quality genome sequence of arenaria genotype A2-O.
α-terthienyl is an allelochemical derived from the roots of marigold (Tagetes spp.), which used to suppress plant parasitic nematodes. We investigated nematicidal activity against model organism Caenorhabditis elegans and root-knot nematode, Meloidogyne incognita. As reported previously, action was much higher after photoactivation, but still effective C. dauer larvae M. incognita second stage juveniles, even without photoactivation. Expression induction two major enzymes, glutathione...
Root-knot nematodes (RKNs) are among the most devastating pests in agriculture. Solanum torvum Sw. (Turkey berry) has been used as a rootstock for eggplant (aubergine) cultivation because of its resistance to RKNs, including Meloidogyne incognita and M. arenaria . We previously found that pathotype , A2-J, is able infect propagate S. torvum. In vitro infection assays showed induced accumulation brown pigments during avirulent A2-O infection, but not virulent A2-J infection. This experimental...
The vacuolar (H+ )-ATPases (V-ATPases) are ATP-driven proton pumps with multiple functions in many organisms. In this study, we performed structural and functional analysis of vha55 gene that encodes V-ATPase subunit B the smokybrown cockroach Periplaneta fuliginosa (Blattodea). We observed a high homology score deduced amino acid sequences between 10 species seven orders. RNAi P. caused nymphal/nymphal molting defects incomplete shedding old cuticles, growth inhibition, as well bent...
The smokybrown cockroach Periplaneta fuliginosa has spread all over the world, and is now one of most undesired invasive alien pests in Japan. Because cockroaches are generally infected by thelastomatid nematodes, they being distributed around world with their parasitic nematodes. Nothing known about nematode species P. differences, or similarity parasite's population structures between different countries host cockroaches. Here we investigated to Japan found that 100% individuals were...
Abstract The Gram-negative bacterium Photorhabdus luminescens which symbiotically associates with the entomopathogenic nematode Heterorhabditis bacteriophora , has a broad insecticidal and nematicidal activity. virulence of P. toward non-mutualistic Caenorhabditis elegans not been described. We showed that when fed on intestinal cells C. worms become delicate some crystal-like structure was developed within lumen. Next, we examined requirement p38 mitogen-activated protein kinase (MAPK)...
Photorhabdus luminescens is a Gram-negative entomopathogenic bacterium which symbiotically associates with the nematode Heterorhabditis bacteriophora P. highly virulent to many insects and nonsymbiotic nematodes, including Caenorhabditis elegans To understand virulence mechanisms of luminescens, we obtained virulence-deficient -attenuated mutants against C. through transposon-mutagenized library. From genetic screening, identified pdxB gene, encoding erythronate-4-phosphate dehydrogenase, as...
Orobanche foetida Poiret is the main constraint facing faba bean crop in Tunisia. Indeed, heavily infested fields with this parasitic plant, yield losses may reach 90%, and recent estimation of area around 80,000 ha. Identifying genes involved Vicia / O . interaction crucial for development effective breeding programs. However, there currently no available information on transcriptome responding to parasitism. In study, we employed RNA sequencing explore global gene expression changes...
Using the primary culture system of male Xenopus laevis hepatocytes consisting more than 95% parenchymal cells, effect estradiol-17 β (10-6 M) on protein synthesis was quantitatively analyzed by 3 H-leucine incorporation kinetics and estimation specific radioactivity newly synthesized secretory protein. The cells in a well defined revealed high plating efficiency very low DNA synthetic activity. cultured could synthesize several proteins containing serum albumin. pattern secreted...
Abstract Background How various host–parasite combinations have been established is an important question in evolutionary biology. We previously described two nematode species, Rhigonema naylae and Travassosinema claudiae , which are parasites of the xystodesmid millipede Parafontaria laminata Aichi Prefecture, Japan. belongs to superfamily Rhigonematoidea, exclusively consists millipedes. T. Thelastomatoidea, includes a wide variety species that parasitize many invertebrates. These...
Temperature-sensitive sporulation mutants were isolated spontaneously from Bacillus subtilis 168 TT by a sequential transfer method. A representative mutant strain, ts32, was characterized in detail. The grew normally at 30°C and 42°C, but did not sporulate 42°C. Electron microscopic observation physiological analysis showed that the blocked stage 0-1 ofsporulation. Genetic suggested mutation located spo0B locus on B. chromosome. Temperature-shift experiments clearly gene product functions...
Photorhabdus luminescens is a gram-negative luminescent enterobacterium that symbiotically resides in the digestive tract of an entomopathogenic nematode (EPN) family Heterorhabditidae. Heterorhabditis bacteriophora upon entering insect host, regurgitate P. cells, and bacteria quickly establish lethal septicemia. To expand possibility its cockroach control, insecticidal activity this nematode-bacterium complex against cockroaches was investigated two ways. First, we examined H. harboring...
ABSTRACT Root-knot nematodes (RKNs) are among the most devastating pests in agriculture. Solanum torvum Sw. (turkey berry) has been used as a rootstock for eggplant (aubergine) cultivation because of its resistance to RKNs, including Meloidogyne incognita and M. arenaria . We previously found that pathotype , A2-J, is able infect propagate S. torvum. In vitro infection assays showed induces accumulation brown pigments during avirulent A2-O infection, but not virulent A2-J infection. This...
The effect of estradiol-17 beta on RNA synthesis and the amounts total polyadenylic acid were determined in primary cultures Xenopus laevis liver parenchymal cells. Results showed that estradiol did not alter content significantly; control cells contained 11.9 +/- 0.34 micrograms estradiol-treated 12.4 0.17 per 10(6) day 2 treatment, 22.0 0.61 24.0 1.09 5. Hybridization with [3H]poly(U) revealed increased poly(A) about 1.2-fold more than controls 1.6-fold 5 treatment. actual rate was...
Journal Article Isolation and Characterization of a Temperature-sensitive Sporulation Mutant Bacillus subtilis Get access Kazuki Sato, Sato Department Biology, Faculty Science, Hiroshima University, 730, Japan Search for other works by this author on: Oxford Academic Google Scholar Hiroyuki Anaguchi, Anaguchi Komei Kobayashi, Kobayashi Taeko Morinaga, Morinaga Naoyuki Nishimura, Nishimura Yasuo Agricultural Biological Chemistry, Volume 48, Issue 8, 1 August 1984, Pages 2047–2053,...