A5067467084- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Paleontology and Stratigraphy of Fossils
- Mechanical stress and fatigue analysis
- Offshore Engineering and Technologies
- Insect Utilization and Effects
- Geological and Geophysical Studies
- Insect and Arachnid Ecology and Behavior
- Geological and Geochemical Analysis
- Paleontology and Evolutionary Biology
- Geochemistry and Geologic Mapping
- Animal Behavior and Reproduction
- Geology and Paleoclimatology Research
- Insect and Pesticide Research
- Antimicrobial Peptides and Activities
- Helicobacter pylori-related gastroenterology studies
- Salivary Gland Tumors Diagnosis and Treatment
- Plant Diversity and Evolution
- Aquaculture disease management and microbiota
- Inflammatory Bowel Disease
- Evolution and Paleontology Studies
- Colorectal and Anal Carcinomas
- Acute Myeloid Leukemia Research
- Cancer Diagnosis and Treatment
- Ear and Head Tumors
The University of Tokyo
2011-2024
Salk Institute for Biological Studies
2020-2022
Yokohama City University
2021-2022
Yokohama City University Medical Center
2021-2022
University of California, San Diego
2020
Tokyo University of Science
1989-2020
Kin-ikyo Chuo Hospital
2019
Furukawa Electric (Japan)
2013
Yamagata University
2009-2011
University of Yamanashi
2008-2011
Inactivation of the ubiquitin ligase Ube3a causes developmental disorder Angelman syndrome, whereas increased dosage is associated with autism spectrum disorders. Despite enriched localization in axon terminals including presynapses, little known about presynaptic function and mechanisms underlying its localization. We show that synapse elimination requires activity Drosophila neurons. further identified domain required for interaction kinesin motor. syndrome-associated missense mutations...
Although painful stimuli elicit defensive responses including escape behavior for survival, starved animals often prioritize feeding over even in a noxious environment. This behavioral priority is typically mediated by suppression of inputs through descending control the brain, yet underlying molecular and cellular mechanisms are incompletely understood. Here we identify cluster GABAergic neurons Drosophila larval designated as SEZ-localized Descending (SDGs), that project axons onto axon...
In the blood (hemolymph) of silkworm Bombyx mori, insect cytokine paralytic peptide (PP) is converted from an inactive precursor to active form in response cell wall components microorganisms and contributes resistance infection. To investigate molecular mechanism underlying up-regulation host induced by PP, we performed oligonucleotide microarray analysis on RNA cells (hemocytes) fat body tissues larvae injected with PP. Expression levels a large number immune-related genes increased...
In the present study, we examined whether microorganisms collaterally ingested by insects with their food activate innate immune system to confer systemic resistance against subsequent bacterial invasion. Silkworms orally administered heat-killed Pseudomonas aeruginosa cells showed intra-hemolymph infection P. aeruginosa. Oral administration of peptidoglycans, cell wall components aeruginosa, conferred protective effects infection, whereas oral lipopolysaccharides, surface components, did...
Sleep-wakefulness regulation dynamically evolves along development in flies, fish, and humans. While the mechanisms regulating sleep adults are relatively well understood, little is known about their counterparts early developmental stages. Here, we report a neuropeptidergic circuitry that modulates developing Drosophila larvae. Through an unbiased screen, identified neuropeptide Hugin its receptor PK2-R1 as critical regulators of larval sleep. Our data suggest HugPC neurons secrete peptides...
Somatosensory neurons (SSNs) that detect and transduce mechanical, thermal, chemical stimuli densely innervate an animal’s skin. However, although epidermal cells provide the first point of contact for sensory stimuli. our understanding roles play in SSN function, particularly nociception, remains limited. Here, we show stimulating Drosophila elicits activation SSNs including nociceptors triggers a variety behavior outputs, avoidance escape. Further, find are intrinsically mechanosensitive...
The insect cytokine paralytic peptide (PP) induces muscle contraction in silkworm larvae. Here we demonstrate that bacterial and fungal cell wall components peptidoglycan glucan stimulate via activation of PP the hemolymph. Anti-PP antibody suppressed induced by PP, peptidoglycan, or glucan. was also inhibited free radical scavengers serine protease inhibitors. Moreover, injecting live silkworms with generated active form PP. produced vitro when incubated hemolymph containing precursor....
Porphyromonas gingivalis, a pathogen that causes inflammation in human periodontal tissue, killed silkworm (Bombyx mori, Lepidoptera) larvae when injected into the blood (hemolymph). Silkworm lethality was not rescued by antibiotic treatment, and heat-killed bacteria were also lethal. Heat-killed of mutant P. gingivalis strains lacking virulence factors silkworms. Silkworms died after injection peptidoglycans purified from (pPG), pPG toxicity blocked treatment with mutanolysin,...
Studies of enterohemorrhagic Escherichia coli (EHEC) infection mechanisms using mammals require large numbers animals and are both costly associated with ethical problems. Here, we evaluated the pathogenic EHEC in silkworm model. Injection a clinically isolated O157:H7 Sakai into either hemolymph or intraperitoneal fluid mice killed host animals. deletion mutants rfbE gene, which encodes perosamine synthetase, monosaccharide component synthetase O-antigen, waaL O-antigen ligase against lipid...
We performed a genomewide analysis using next-generation sequencer to investigate the effect of pulmonary surfactant on gene expression in Staphylococcus aureus, clinically important opportunistic pathogen. RNA sequence (RNA-seq) bacterial transcripts at late log phase revealed 142 genes that were upregulated >2-fold following addition culture medium. Among these genes, we confirmed by quantitative reverse transcription-PCR mRNA amounts for encoding ESAT-6 secretion system C (EssC), an...
Inter-male aggressive behavior is a prominent sexually dimorphic behavior. Neural circuits that underlie are therefore likely under the control of sex-determining genes. However, neurogenetic mechanism generates sex-specific remains largely unknown. Here, we found neuronal class specified by one Drosophila sex determining genes, fruitless (fru), belongs to neural circuit male-type This can promote independent another gene, doublesex (dsx), although dsx involved in ensuring performed only...
For successful mating, a male animal must execute effective courtship behaviors toward receptive target sex, which is female. Whether the execution capability and upregulation of females are specified through separable sex-determining genetic pathways remains uncharacterized. Here, we found that one two Drosophila genes, doublesex (dsx), specifies male-specific neuronal component serves as an mechanism for behavior, whereas fruitless (fru) required enhancement behavior females. The...
Abstract The development of vancomycin (VCM) resistance in Staphylococcus aureus threatens global health. Studies the VCM-resistance mechanism and alternative therapeutic strategies are urgently needed. We mutagenized S. laboratory strains methicillin-resistant (MRSA) with ethyl methanesulfonate isolated mutants that exhibited high to VCM (minimum inhibitory concentration = 32 μg/ml). These VCM-resistant were sensitive linezolid rifampicin partly arbekacin daptomycin. Beta-lactams had...