A5076302608- Oral microbiology and periodontitis research
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Oral and gingival health research
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Infective Endocarditis Diagnosis and Management
- Oral Health Pathology and Treatment
- HIV/AIDS oral health manifestations
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- Biochemical and Structural Characterization
- Bone and Dental Protein Studies
- Mycobacterium research and diagnosis
- Antimicrobial Peptides and Activities
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Trace Elements in Health
- Escherichia coli research studies
- Oral health in cancer treatment
- Enzyme Structure and Function
Nagasaki University
2016-2025
Kyoto University
2023
Kyushu University
2002-2011
The University of Tokyo
2010
Charité - Universitätsmedizin Berlin
2010
RIKEN Center for Integrative Medical Sciences
2010
Biology of Infection
2005-2010
Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Protein secretion systems common to other Gram-negative bacteria lacking P. gingivalis, but several proteins, including PorT, have been linked gingipain secretion. Comparative genome analysis and genetic experiments revealed 11 additional proteins involved Six of these (PorK, PorL, PorM, PorN, PorW, Sov) were similar sequence Flavobacterium johnsoniae gliding motility two...
The gram-negative anaerobic bacterium Porphyromonas gingivalis is a major causative agent of chronic periodontitis. strains have been classified into virulent and less-virulent by mouse subcutaneous soft tissue abscess model analysis. Here, we present the whole genome sequence P. ATCC 33277, which as strain. We identified 2090 protein-coding sequences (CDSs), 4 RNA operons, 53 tRNA genes in 33277 genome. By genomic comparison with strain W83, 461 33277-specific 415 W83-specific CDSs....
The anaerobic Gram-negative bacterium Porphyromonas gingivalis is a major pathogen in severe forms of periodontal disease and refractory periapical perodontitis. We have recently found that P. has novel secretion system named the Por (PorSS), which responsible for extracellular proteinases, Arg-gingipains (Rgps) Lys-gingipain. These proteinases contain conserved C-terminal domains (CTDs) their C-termini. Hemin-binding protein 35 (HBP35), one outer membrane proteins contributes to its haem...
The dual membrane envelopes of Gram-negative bacteria provide two barriers unlike nature that regulate the transport molecules into and out organisms. Organisms have developed several systems for across inner outer membranes. periodontopathogenic bacterium Porphyromonas gingivalis produces proteinase adhesin complexes, gingipains/adhesins, on cell surface in extracellular milieu as one major virulence factors. Gingipains and/or adhesins are encoded by kgp, rgpA, rgpB, hagA chromosome. In...
Abstract The periodontopathogen Porphyromonas gingivalis secretes potent pathogenic proteases, gingipains, via the type IX secretion system (T9SS). This comprises at least 11 components; however, regulatory mechanism of their expression has not yet been elucidated. Here, we found that PorY (PGN_2001)-PorX (PGN_1019)-SigP (PGN_0274) cascade is involved in regulation T9SS. Surface plasmon resonance (SPR) analysis revealed a direct interaction between recombinant (rPorY) and PorX (rPorX). rPorY...
Porphyromonas gingivalis (Pg) infection causes periodontal disease and exacerbates rheumatoid arthritis (RA). It is reported that inoculation of periodontopathogenic bacteria (i.e., Pg) can alter gut microbiota composition in the animal models. Gut dysbiosis human has shown strong associations with systemic diseases, including RA, diabetes mellitus, inflammatory bowel disease. Therefore, this study investigated dysbiosis-mediated by Pg oral an experimental model mouse.Pg cavity twice a week...
The periodontopathogen Porphyromonas gingivalis is an obligate anaerobe that devoid of catalase but exhibits a relatively high degree resistance to peroxide stress. In the present study, we demonstrate P. contains Dps homologue plays important role in protection cells from protein isolated displayed ferritin-like spherical polymer consisting 19-kDa subunits. Molecular cloning and sequencing gene encoding this revealed it had similarity nucleotide amino acid sequences proteins other species....
Porphyromonas gingivalis is an oral pathogen primarily associated with severe periodontal disease and further rheumatoid arthritis, dementia, cardiovascular disease, certain cancers. Protein glycosylation can be important for a variety of reasons including protein function, solubility, protease resistance, thermodynamic stability.
Porphyromonas gingivalis, the bacterium responsible for periodontitis, produces several pathogenic factors, including methyl mercaptan, which contribute to disease. Kouboku (Magnoliaceae), a Chinese herbal medicine, has been shown suppress mercaptan production from P. gingivalis. In this study, we investigated inhibitory effect of on production, biofilm formation, gingivalis-host cell interactions, and its potential synergistic antibacterial with antibiotics. Five standard five clinically...
A nonpigmented mutant of Porphyromonas gingivalis was constructed by using transposon mutagenesis. The possessed the DNA at novel gene porR. Gene targeted mutagenesis revealed that porR responsible for pigmentation. shared similarities with genes degT family, products which are now considered to be transaminases involved in biosynthesis sugar portions cell-surface polysaccharides and aminoglycosides. showed a pleiotropic phenotype: delayed maturation fimbrillin, preferential presence Rgp Kgp...
Evidence from recent epidemiological studies suggests a link between periodontal infections and increased risk of atherosclerosis related cardiovascular cerebrovascular events in human subjects. One the major pathogens periodontitis, Porphyromonas gingivalis, has ability to aggregate platelets platelet-rich plasma (PRP). Mechanism P. gingivalis-induced platelet aggregation PRP was investigated. Proteinase inhibitors toward Arg-gingipain (Rgp) Lys-gingipain (Kgp) did not suppress PRP, whereas...
Summary Bacterial cell surface filaments play significant roles in adherence to and invasion of host cells. They are generated by the chaperone/usher pathway system (class I fimbriae), type II secretion (type IV pili) nucleation‐dependent polymerization (Curli filaments) that categorized their modes expression assembly. In this study, we found periodontal pathogen Porphyromonas gingivalis expressed major structural components two (fimbrilin 75 kDa protein) had extremely long prosequences...
Porphyromonas gingivalis and Treponema denticola are major pathogens of periodontal disease. Coaggregation between microorganisms plays a key role in the colonization gingival crevice organization periodontopathic biofilms. We investigated involvement surface ligands P. coaggregation. Two triple mutants lacking Arg-gingipain A (RgpA), Lys-gingipain (Kgp) Hemagglutinin (HagA) or RgpA, B (RgpB) Kgp showed significantly decreased coaggregation with T. denticola, whereas fimbriae...
Porphyromonas gingivalis, a pathogen that causes inflammation in human periodontal tissue, killed silkworm (Bombyx mori, Lepidoptera) larvae when injected into the blood (hemolymph). Silkworm lethality was not rescued by antibiotic treatment, and heat-killed bacteria were also lethal. Heat-killed of mutant P. gingivalis strains lacking virulence factors silkworms. Silkworms died after injection peptidoglycans purified from (pPG), pPG toxicity blocked treatment with mutanolysin,...
Abstract Background The periodontal pathogen Porphyromonas gingivalis is an obligate anaerobe that requires heme for growth. To understand its acquisition mechanism, we focused on a hemin-binding protein (HBP35 protein), possessing one thioredoxin-like motif and conserved C-terminal domain, which are proposed to be involved in redox regulation cell surface attachment, respectively. Results We observed the hbp35 gene was transcribed as 1.1-kb mRNA with subsequent translation resulting three...
Tannerella forsythia , a Gram-negative anaerobic bacterium, is an important pathogen in periodontal disease. This bacterium possesses genes encoding all known components of the type IX secretion system (T9SS). T. mutants deficient orthologous to T9SS-encoding porK porT and sov were constructed. All single lacked surface layer (S-layer) expressed less-glycosylated versions S-layer glycoproteins TfsA TfsB. In addition, these exhibited decreased haemagglutination increased biofilm formation....
Abstract The periodontal pathogen Porphyromonas gingivalis has two different lipopolysaccharide (LPS) molecules, O-LPS and A-LPS. We have recently shown that P. strain HG66 lacks Here, we found introduction of a wild-type wbpB gene into restored formation Sequencing the from revealed presence nonsense mutation in gene. product is member Wbp pathway, which plays role synthesis UDP-ManNAc(3NAc)A Pseudomonas aeruginosa ; sequentially synthesized by WbpA, WbpB, WbpE, WbpD WbpI proteins. then...
Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins cell surface where they are anchored via glycolipids. In P. gingivalis, glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure. Modified were deglycosylated using trifluoromethanesulfonic acid digested with trypsin or proteinase K. The residual modifications then extensively analyzed by tandem mass spectrometry. C terminus each protein was amide-bonded a...
Abstract The type IX secretion system (T9SS) was originally discovered in Porphyromonas gingivalis , one of the pathogenic bacteria associated with periodontal disease and is now known to be present many members phylum Bacteroidetes . T9SS secretes a number potent virulence factors, including highly hydrolytic proteases called gingipains, across outer membrane P. To understand entire machinery T9SS, an exhaustive search for T9SS‐related genes using mariner family transposon (Tn) Tn‐seq...
Abstract Porphyromonas gingivalis is a Gram‐negative anaerobic bacterium that has been considered to be one of the bacteria associated with progression human periodontitis. Subgingival biofilms formed by bacteria, including P. , induce chronic inflammation, and activation inflammasome in gingival tissue. However, mechanisms gingivalis‐ triggering role bacteria–host interactions are controversial. In this study, we investigated potential for cells mouse models. We demonstrated secreted or...