A5077736337- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Hematopoietic Stem Cell Transplantation
- Cell death mechanisms and regulation
- Hepatitis B Virus Studies
- Advanced Biosensing Techniques and Applications
- Rheumatoid Arthritis Research and Therapies
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- HER2/EGFR in Cancer Research
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Clostridium difficile and Clostridium perfringens research
- Vector-Borne Animal Diseases
University of Toyama
2014-2024
Birla Institute of Technology and Science - Hyderabad Campus
2023
Birla Institute of Technology and Science, Pilani
2023
Brotman Baty Institute
2023
Zero to Three
2009-2018
Hiroshima University Hospital
2015
Dana-Farber Cancer Institute
2015
Hiroshima University
2015
Toyama College
2006
Abstract Background SARS-CoV-2 is a novel coronavirus that emerged in 2019 and now classified the genus Coronavirus with closely related SARS-CoV. highly pathogenic humans as biosafety level (BSL)-3 pathogen, which makes manipulating it relatively difficult due to its infectious nature. Methods To circumvent need for BSL-3 laboratories, an alternative assay was developed avoids live virus instead uses recombinant VSV expressing luciferase possesses full length or truncated spike proteins of...
Abstract Following genomics and proteomics, cytomics, a novel method of looking at life, has emerged for analyzing large populations cells on single‐cell basis with multiple parameters in quantitative manner. We have developed highly integrated live‐cell microarray system the cellular responses individual using microwell array chip that 234,000 microwells each which is just enough to fit single cell. Compared flow cytometry microscope‐based methods, our can analyze history number cells....
Porphyromonas gingivalis (Pg) infection causes periodontal disease and exacerbates rheumatoid arthritis (RA). It is reported that inoculation of periodontopathogenic bacteria (i.e., Pg) can alter gut microbiota composition in the animal models. Gut dysbiosis human has shown strong associations with systemic diseases, including RA, diabetes mellitus, inflammatory bowel disease. Therefore, this study investigated dysbiosis-mediated by Pg oral an experimental model mouse.Pg cavity twice a week...
Abstract G-protein-coupled receptors (GPCRs) are one of the most important drug targets and anti-GPCR monoclonal antibody (mAb) is an essential tool for functional analysis GPCRs. However, it very difficult to develop GPCR-specific mAbs due difficulties in production recombinant GPCR antigens lack efficient mAb screening method. Here we describe a novel approach against using two original methods, bilayer-dialysis method biotinylated liposome-based interaction assay (BiLIA), both which...
The highly conserved matrix protein 2 (M2) is a good candidate for the development of broadly protective influenza vaccine that induces long-lasting immunity. In animal models, natural killer (NK) cells have been proposed to play an important role in protection provided by M2-based vaccines through mechanism antibody-dependent cell-mediated cytotoxicity (ADCC). We investigated ability human anti-M2 Ab1-10 monoclonal antibody (mAb) activate NK cells. They mediated ADCC against M2-expressing...
Since its discovery over three decades ago, signal transducer and activator of transcription 1 (STAT1) has been extensively studied as a central mediator for interferons (IFNs) signaling antiviral defense. Here, using genetic biochemical assays, we unveil Thr 748 conserved IFN-independent phosphorylation switch in Stat1, which restricts IFN promotes innate inflammatory responses following the recognition bacterial-derived toxin lipopolysaccharide (LPS). Genetically engineered mice expressing...
Abstract T-cell receptor (TCR) gene therapy is a promising next-generation antitumor treatment. We previously developed single–T-cell analysis protocol that allows the rapid capture of paired TCRα and β cDNAs. Here, we applied to analyze TCR repertoire tumor-infiltrating lymphocytes (TIL) various cancer patients. found clonally expanded populations T cells expressed same clonotypic in 50% 70% CD137+CD8+ TILs, indicating they responded certain antigens tumor environment. To assess reactivity...
Hematopoietic stem cells (HSCs) that lack HLA-class I alleles as a result of copy-number neutral loss heterozygosity the short arm chromosome 6 (6pLOH) or HLA allelic mutations often constitute hematopoiesis in patients with acquired aplastic anemia (AA), but precise mechanisms underlying clonal induced by these HLA-lacking (HLA-) HSCs remain unknown. To address this issue, we generated pluripotent (iPSCs) from an AA patient who possessed HLA-B4002-lacking (B4002-) leukocytes. Three...
Many potent neutralizing SARS-CoV-2 antibodies have been developed and used for therapies. However, the effectiveness of many has reduced against recently emerging variants, especially Omicron variant. We identified a highly antibody, UT28K, in COVID-19 convalescent individuals who recovered from severe condition. UT28K showed efficacy an
The segmented RNA genome of influenza A viruses (IAVs) enables viral evolution through genetic reassortment after multiple IAVs coinfect the same cell, leading to harboring combinations eight genomic segments from distinct parental viruses. Existing data indicate that reassortant genotypes are not equiprobable; however, low throughput available virology techniques does allow quantitative analysis. Here, we have developed a high-throughput single-cell droplet microfluidic system allowing...
Abstract The authors previously developed a cell‐microarray system that effectively detects antigen‐specific B‐cells by monitoring intracellular Ca 2+ at single cell levels. Here they present novel method to detect using system. To B‐cells, arrayed live lymphocytes on chip, stained cells with fluorescence‐labeled nonspecific proteins, and analyzed them fluorescence scanner protein binding B‐cells. They then antigen the scanner. Cells specific antigen, but not were determined as harvested....
T-cell receptor (TCR) α/β chains are expressed on the surface of CD8+ T-cells and have been implicated in antigen recognition, activation, proliferation. However, methods for characterization human TCRα/β not well established largely because complexity their structures owing to extensive genetic rearrangements that they undergo. Here we report development an integrated 5′-RACE multiplex PCR method amplify full-length transcripts at single-cell level subsets, including naive, central memory,...
Objective To investigate the reactivity of monoclonal anti–citrullinated protein antibody (ACPA) obtained from peripheral blood B cells rheumatoid arthritis (RA) patients with human autoantigens as well environmental proteins by determining essential epitope for ACPA. Methods A ACPA (cyclic citrullinated peptide 1 [CCP‐Ab1]) was screening lymphocytes 31 RA using a novel antibody–secreting cell (ASC) system, immunospot‐array assay on chip. The CCP‐Ab1 determined mapping. Then, human,...
Abstract Tyrosine kinase activity of the asymmetric EGFR homodimer is negatively regulated via ERK-mediated phosphorylation Thr-669 in juxtamembrane domain. In present study, we investigated human breast cancer cells whether a similar mechanism plays role feedback regulation ErbB2/ErbB3 heterodimer, most potent ErbB receptor dimer. Constitutive tyrosine ErbB2 and ErbB3 was significantly decreased phorbol ester- growth factor-treated BT-474 MDA-MB-453 cells. contrast to phosphorylation,...
Leukocytes that lack HLA allelic expression are frequently detected in patients with acquired aplastic anemia (AA) who respond to immunosuppressive therapy (IST), although the exact mechanisms underlying loss and allele repertoire likely acquire loss-of-function mutations unknown. We identified a common nonsense mutation at position 19 (c.19C>T, p.R7X) exon 1 (Exon1mut) of different HLA-A -B alleles HLA-lacking granulocytes from AA patients. A droplet digital PCR (ddPCR) assay capable...