A5069582801- Cancer Genomics and Diagnostics
- Advanced Breast Cancer Therapies
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Esophageal Cancer Research and Treatment
- Esophageal and GI Pathology
- Kruppel-like factors research
- Immunotherapy and Immune Responses
- Metastasis and carcinoma case studies
- Cancer Treatment and Pharmacology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- MicroRNA in disease regulation
- Gastrointestinal Tumor Research and Treatment
- Colorectal Cancer Treatments and Studies
- Cancer Diagnosis and Treatment
- Cancer-related gene regulation
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Drug Solubulity and Delivery Systems
- Lung Cancer Research Studies
- Fibroblast Growth Factor Research
- Neuroendocrine Tumor Research Advances
Toho University Ohashi Medical Center
2021-2025
Toho University
2021-2025
Showa University
2021-2024
Showa University Koto Toyosu Hospital
2021-2024
Showa University Hospital
2021-2024
Kansai University
2000-2023
University Hospital and Clinics
2023
University Medical Center
2023
Toyo University
2023
University of Toyama
2012-2021
BACKGROUND: Adequate blood supply for the reconstructed organ is important safe esophagogastric anastomosis during esophagectomy. Recently, indocyanine green (ICG) has been used visualization of when performed in vascular surgery. To visualize reconstruction, we employed ICG fluorescence METHODS: From August 2008, 40 patients received cervical or thoracic They consisted 33 having esophagectomy esophageal cancer, 3 being treated and 4 with double cancer regions. Before after pulling up organ,...
To identify genetic events that characterize cancer progression, we conducted a comprehensive evaluation of 161 primary breast tumors. Similar to the "mountain-and-hill" view mutations, gene amplification also shows high- and low-frequency alterations in cancers. The frequently amplified genes include well-known oncogenes ERBB2, FGFR1, MYC, CCND1, PIK3CA, whereas other known are amplified, although less frequently, CCND2, EGFR, FGFR2, NOTCH3. More importantly, by honing on minimally regions...
Colorectal cancer is a major cause of cancer-related mortality worldwide, with high incidence recurrence following curative resection, particularly among patients stage II and III disease. There therefore need for novel prognostic biomarkers advanced colon it was recently reported that aquaporin-1 (AQP1) may be associated aggressive characteristics cells in experimental data. The association clinicopathological findings AQP1 expression evaluated by tissue microarray (TMA) analysis, to...
Epithelial cell adhesion molecule (EpCAM) is a marker for circulating tumor cells (CTCs) in various types of cancer, while cluster differentiation 44 (CD44) gastric cancer (GC) stem cells. To evaluate the clinical significance CD44+ CTCs patients with GC present study, number EpCAM+CD44+ and EpCAM+CD44- were detected peripheral blood 26 12 healthy volunteers using flow cytometry. The (mean ± standard deviation) was 69.9±52.0 0.91±2.10, respectively (P=0.0001), that 59.1±88.0 9.83±9.91,...
Developing delivery vehicles that achieve drug accumulation in the liver and transferability into hepatic stellate cells (HSCs) across sinusoidal endothelium is essential to establish a treatment for fibrosis. We previously developed hyaluronic acid (HA)-coated polymeric micelles exhibited affinity endothelial cells. HA-coated possess core–shell structure of self-assembled biodegradable poly(l-lysine)-b-poly(lactic acid) AB-diblock copolymer (PLys+-b-PLLA), its exterior coated with HA...
MicroRNA (miRNA) is a small non-coding RNA that targets specific mRNA. Recent progress in the extraction of from formalin-fixed paraffin-embedded (FFPE) tissues has facilitated miRNA profiling using samples stored laboratories worldwide. In present study, gastric cancer patients determined FFPE samples. First, criteria were established for determining evaluable was then undertaken oligo chips with 885 featured genes. The obtained 47 who underwent operations between 1997 and 2007. Results...
Abstract T-cell receptor (TCR) gene therapy is a promising next-generation antitumor treatment. We previously developed single–T-cell analysis protocol that allows the rapid capture of paired TCRα and β cDNAs. Here, we applied to analyze TCR repertoire tumor-infiltrating lymphocytes (TIL) various cancer patients. found clonally expanded populations T cells expressed same clonotypic in 50% 70% CD137+CD8+ TILs, indicating they responded certain antigens tumor environment. To assess reactivity...
Abstract Objective Although cancer therapy suppresses recurrence and prolongs life, it may be accompanied by strong side effects; thus, there is a demand for the development effective treatments with fewer effects. Cancer using plant-derived essential oils attracting attention as one promising method. This study investigated antitumor effects of oil volatiles on breast cells identifies four that display activity. Methods Breast were cultured in 96-well plate, then twenty was added dropwise...
Background/Objectives: In a previous study, we reported novel surgical procedure called the Total Sealing Technique (TST), which uses bipolar vessel-sealing system to reduce lymphatic leakage during axillary dissection for breast cancer. Follow-up ob-servations showed lower incidence of cancer-related lymphedema (BCRL) in patients TST group. Therefore, this current aim investigate and compare BCRL between two groups (TST conventional electrocautery (CONV)) discuss why was significantly group...
The primary goal in treating patients with breast cancer who have positive axillary lymph nodes is to achieve optimal tumor control while minimizing side effects that include cancer-related lymphedema (BCRL) [...]
Despite advances in radical esophagectomies and adjuvant therapy, the postoperative prognosis esophageal squamous cell carcinoma (ESCC) patients remains poor. The aim of this study was to identify a molecular signature predict favorable outcomes with ESCC. As training data set, total RNA extracted from formalin-fixed paraffin-embedded samples surgically removed specimens 19 ESCC who underwent curative esophagectomy. expression microRNA (miRNA) detected using miRNA oligo chip on which 885...
Gallbladder cancer (GBC) is one of the most unfavorable prognostic tumor, and immediate growth distant metastasis are important factors associated with poor prognosis patients this disease. Standard variant isoforms CD44 tumor growth, metastasis, epithelial-mesenchymal transition (EMT), although their roles in GBC unclear. We investigated relationship between EMT, chemotaxis, tumorigenicity. analyzed expression cell line NOZ found that it comprises a major population expressed...
Mitotically quiescent cancer stem cells (CSC) are hypothesized to exhibit a more aggressive phenotype involving greater therapeutic resistance and metastasis. The aim of our study was develop method for identifying CSC in esophageal squamous cell carcinoma (ESCC) based on their expression the p75 neurotrophin receptor (p75NTR) other proposed markers, such as CD44 CD90. Double immunostaining surgical ESCC specimens revealed that mean Ki-67-labeling index p75NTR-positive significantly lower...
The present study evaluated the capture efficiency of esophageal and breast cancer cells with a modified 'polymeric circulating tumor (CTC)-chip' microfluidic device, which was developed for isolation cells. Esophageal cell lines KYSE150, KYSE220 KYSE510, MCF7, SKBR3 MDA-MB-231 were used evaluation. efficiencies in phosphate-buffered saline (PBS) ~0.9, irrespective epithelial adhesion molecule (EpCAM) expression, represented as mean fluorescent intensity from 528 to 76. In lines, efficient...
Mitochondria play a central role in many apoptotic reactions. Although mitochondrial changes and caspase activation have been demonstrated the thymocytes, cell death signal through mitochondria TCR-stimulated thymocytes has not fully understood. In this study, we show that TCR stimulation induced disruption of transmembrane potential (Δ Ψm), cytochrome c release from mitochondira, capase-3 activation, thymocytes. Bongkrekic acid, an inhibitor Δ Ψm disruption, blocked following...
<b><i>Background/Purpose:</i></b> Aquaporins (AQPs) are important in controlling bile formation. However, the exact role human gallbladder carcinogenesis has not yet been defined. <b><i>Methods:</i></b> AQP-5-expressing carcinoma (GBC) cell lines (NOZ) were transfected with anti-AQP-5 small interfering RNA (siRNA). Growth, migration, invasion assay, and drug susceptibility tests performed. Next, microRNA (miRNA) expression was analyzed by miRNA...
MicroRNA (miR)-203 has been shown to induce squamous differentiation of epidermal stem cells through the suppression p63. The aim this study was assess tumor suppressor effect miR-203 in esophageal cell carcinoma (ESCC) with focus on regulation fate decisions and organization tissue architecture vivo. Our investigation establishing stable clones from ESCC lines induced expression resulted significant growth inhibition a mouse xenograft model. Small foci were observed tumors stratified...