A5072734104- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Oral microbiology and periodontitis research
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
- Oral and gingival health research
- dental development and anomalies
- Bone and Dental Protein Studies
- Bone Tissue Engineering Materials
- Oral and Maxillofacial Pathology
- Rheumatoid Arthritis Research and Therapies
- Gut microbiota and health
- Systemic Sclerosis and Related Diseases
- MicroRNA in disease regulation
- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- RNA Research and Splicing
- Salivary Gland Disorders and Functions
- NF-κB Signaling Pathways
- Clostridium difficile and Clostridium perfringens research
- Neuropeptides and Animal Physiology
- Nuclear Receptors and Signaling
- S100 Proteins and Annexins
- Clusterin in disease pathology
Hiroshima University
2016-2025
The Japanese Society of Gastroenterological Surgery
2024
Iwate Medical University
2024
Onomichi General Hospital
2024
Japan Society for the Promotion of Science
2020
Tokushima University
2020
Kure Medical Center
2020
National Hospital Organization
2020
Genomics (United Kingdom)
2020
Hiroshima University Hospital
2018-2020
Endothelins (ETs) are very potent vasoconstrictive peptides and have diverse functions in both vascular nonvascular tissues. This investigation concerns the tissue distribution cellular localization of rat mRNAs encoding two different subtypes ET receptors (ETA ETB). We isolated 46 cDNA clones from a lung library by hybridization with bovine ETA cDNA. The characterization these indicated that they represent either or ETB In situ blot analyses revealed mRNA is predominantly expressed smooth...
Recently, there have been an increased number of basic and clinical reports indicating the superior potential bone marrow-derived mesenchymal stem cells (MSCs) for tissue regeneration. In periodontal treatment, previous animal studies indicated that autotransplantation marrow MSCs into experimental defects enhanced However, mechanisms regeneration with are still unclear. The purpose this study was to elucidate behavior transplanted in defects.Bone were isolated from beagle dogs, labeled...
Transplantation of mesenchymal stem cells (MSCs), which possess self-renewing properties and multipotency, into a periodontal defect is thought to be useful option for tissue regeneration. However, developing more reliable predictable implantation techniques still needed. Recently, we generated clumps an MSC/extracellular matrix (ECM) complex (C-MSC), consisted self-produced ECM. C-MSCs can regulate their cellular functions in vitro grafted site, without any artificial scaffold, induce bone...
Porphyromonas gingivalis (Pg) infection causes periodontal disease and exacerbates rheumatoid arthritis (RA). It is reported that inoculation of periodontopathogenic bacteria (i.e., Pg) can alter gut microbiota composition in the animal models. Gut dysbiosis human has shown strong associations with systemic diseases, including RA, diabetes mellitus, inflammatory bowel disease. Therefore, this study investigated dysbiosis-mediated by Pg oral an experimental model mouse.Pg cavity twice a week...
To address whether brain-derived neurotrophic factor (BDNF) could be involved in periodontal tissue regeneration, we examined the effects of BDNF on proliferation and expression bone (cementum)- related proteins (osteopontin, morphogenetic protein [BMP]-2, type I collagen, alkaline phosphatase [ALPase], osteocalcin) cultures human ligament (HPL) cells, which are thought to prerequisite for angiogenesis endothelial cells. Furthermore, effect regeneration tissues experimentally induced defects...
Background: Periodontal disease (PD) is a risk factor for systemic diseases, including neurodegenerative diseases. The role of the local and inflammation induced by PD in neuroinflammation currently remains unclear. present study investigated involvement periodontal blood-brain barrier (BBB) disruption. Methods: To induce mice (c57/BL6), ligature was placed around second maxillary molar. Periodontal, systemic, were assessed based on inflammatory cytokine mRNA or protein levels using qPCR...
The major problem in cell therapy is the possibility of viral or bacterial infection and immune reactions. Therefore, it expected culture cells which are intended to be re-implanted with autologous serum rather than conventional bovine serum. Cell human mesenchymal stem (hMSC), differentiating various cells, thought curative. To hMSC (HAS) re-implant them for therapy, we developed a completely closed bag system separating serum, comparing proliferation multipotency cultured HAS those foetal...
Brain-derived neurotrophic factor (BDNF), recognized as essential in the developing nervous system, is involved differentiation and proliferation non-neuronal cells, such endothelial osteoblasts, periodontal ligament cells. We have focused on application of BDNF to regeneration tissue indicated that promotes experimentally created defects. Cementoblasts form cementum, mineralized tissue, which key establishing a functional periodontium. The requires elucidation mechanism by regulates...
Cherubism is caused by mutations in SH3BP2. Studies of cherubism mice showed that tumor necrosis factor α (TNF-α)-dependent autoinflammation a major cause the disorder but failed to explain why human lesions are restricted jaws and regress after puberty. We demonstrate inflammation MYD88 dependent rescued absence TLR2 TLR4. However, germ-free also develop inflammation. Mutant macrophages hyperresponsive PAMPs (pathogen-associated molecular patterns) DAMPs (damage-associated activate...
RNA-binding proteins (RBPs) regulate mRNA stability by binding to the 3'-untranslated region (UTR) of mRNA. Human antigen-R (HuR), one RBPs, is involved in progression diseases, such as rheumatoid arthritis, diabetes mellitus and some inflammatory diseases. Interleukin (IL)-6 a major cytokine regulated HuR Periodontal disease (PD) also an caused elevations IL-6 following infection periodontopathogenic bacteria. The involvement PD was assessed using in-vitro in-vivo experiments....
This study aimed to investigate the factors influencing clinical outcomes of regenerative therapy using recombinant human fibroblast growth factor-2 (rhFGF-2).
Porphyromonas gingivalis, the bacterium responsible for periodontitis, produces several pathogenic factors, including methyl mercaptan, which contribute to disease. Kouboku (Magnoliaceae), a Chinese herbal medicine, has been shown suppress mercaptan production from P. gingivalis. In this study, we investigated inhibitory effect of on production, biofilm formation, gingivalis-host cell interactions, and its potential synergistic antibacterial with antibiotics. Five standard five clinically...
Epidemiological studies have linked periodontitis to rheumatoid arthritis (RA). Porphyromonas gingivalis (Pg) was reported recently produce citrullinated protein (CP) and increase anti-cyclic CP antibody (ACPA), both of which been identified as causative factors RA. In the present study, we determined effects Pg infection on exacerbation RA in a mouse model. model mice (SKG mice) were established by an intraperitoneal (i.p.) injection laminarin (LA). Mice divided into six groups, Ctrl (PBS...
Three-dimensional cultured clumps of a mesenchymal stem cell (MSC)/extracellular matrix (ECM) complex (C-MSC) consists cells and self-produced ECM. C-MSC can regulate the cellular function in vitro induce successful bone regeneration using ECM as scaffold. Potentiating immunomodulatory capacity C-MSCs, which ameliorate allo-specific immune response, may be helpful developing beneficial "off-the-shelf" therapy for tissue regeneration. It is well reported that interferon (IFN)-γ stimulates...
The purpose of this study is to evaluate whether β-tricalcium phosphate (β-TCP) could be a promising modality help augment alveolar bone in periodontal tissue regeneration by marrow mesenchymal stem cells (BMMSCs).Expanded BMMSCs and atelocollagen (Col) were mixed together (MSC/Col). A combination β-TCP with MSC/Col was also prepared (MSC/Col/TCP). MSC/Col/TCP or transplanted into experimental Class III furcation defects that had been exposed inflammation beagle dogs. Periodontal evaluated...
Three-dimensional clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist cells and self-produced ECM. We demonstrated previously that C-MSCs can be transplanted into bone defect regions with no artificial scaffold to induce regeneration. To apply in a clinical setting as reliable regenerative therapy, the present study aimed generate xeno-free/serum-free conditions exert successful properties monitor interactions between grafted host during healing...
Epidemiological evidence to suggest that periodontal disease (PD) is involved in the progression of rheumatoid arthritis (RA) increasing. The complement system plays a critical role immune responses. C5a has been implicated chronic inflammatory diseases, including PD and RA. Porphyromonas gingivalis major causative bacteria can produce C5a. Therefore, it hypothesized P. infection RA by elevating levels. In present study, gingivalis–infected model mice were established investigate involvement...
Molecular gene markers, which can distinguish human bone marrow mesenchymal stem cells from fibroblasts, have recently been reported. Messenger RNA levels of tissue factor pathway inhibitor-2, major histocompatibility complex-DR-alpha, complex-DR-beta, and neuroserpin are higher in than fibroblasts. However, express less apolipoprotein D mRNA Periodontal ligament a heterogeneous cell population including cells, progenitor osteoblasts or cementoblasts. The use molecular markers that...