A5101778624- Oral microbiology and periodontitis research
- Bone and Dental Protein Studies
- Mesenchymal stem cell research
- Nerve injury and regeneration
- Oral and Maxillofacial Pathology
- Signaling Pathways in Disease
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- dental development and anomalies
- Salivary Gland Disorders and Functions
- Inflammatory mediators and NSAID effects
- Immune Response and Inflammation
- Protease and Inhibitor Mechanisms
- HIV/AIDS oral health manifestations
- Biosensors and Analytical Detection
- Renal and related cancers
- Contact Dermatitis and Allergies
- Salivary Gland Tumors Diagnosis and Treatment
- Oral and gingival health research
- Barrier Structure and Function Studies
- Dental Trauma and Treatments
- Cardiac Fibrosis and Remodeling
- Ear and Head Tumors
- Intracerebral and Subarachnoid Hemorrhage Research
- TGF-β signaling in diseases
Hiroshima University Hospital
2012-2023
Hiroshima University
2006-2022
Oral squamous-cell carcinoma (OSCC) is one of the most common types human cancer. Typically OSCC cells show persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. We previously identified Periostin as gene demonstrating highest fold change expression in invasive clone by comparing transcriptional profile parent cell line a highly clone. Here, we demonstrated overexpression enhanced invasiveness oral cancer lines. To know role invasion, angiogenesis...
Background Epidemiological studies have revealed a link between dental infection and preterm birth or low weight (PTB/LBW), however, the underlying mechanisms remain unclear. Progress in understanding associated has been limited part by lack of an animal model for chronic infection-induced PTB/LBW, mimicking pregnancy under conditions periodontitis. We aimed to establish mouse periodontitis order investigate PTB/LBW. Methods To inflammation beginning with infection, we surgically opened...
The purpose of this study was to investigate the effects low-intensity pulsed ultrasound (LIPUS) stimulation on proliferation and differentiation cementoblast lineage cells.An immortalized human periodontal ligament cell line (HPL) showing immature cementoblastic used. Cultured HPL cells were subjected LIPUS exposure (frequency = 1 MHz; 1:4; intensity 30 mW/cm(2)) or sham for 15 minutes per day. Expression levels alkaline phosphatase (ALP), type I collagen (Col-I), runt-related gene 2...
Brain-derived neurotrophic factor (BDNF), recognized as essential in the developing nervous system, is involved differentiation and proliferation non-neuronal cells, such endothelial osteoblasts, periodontal ligament cells. We have focused on application of BDNF to regeneration tissue indicated that promotes experimentally created defects. Cementoblasts form cementum, mineralized tissue, which key establishing a functional periodontium. The requires elucidation mechanism by regulates...
Background A number of studies have revealed a link between chronic periodontitis and cardiovascular disease in obese patients. However, there is little information about the influence periodontitis-associated bacteria, Porphyromonas gingivalis (Pg), on pathogenesis atherosclerosis obesity. Methods In vivo experiment: C57BL/6J mice were fed with high-fat diet (HFD) or normal chow (CD), as control. Pg was infected from pulp chamber. At 6 weeks post-infection, histological immunohistochemical...
Our previous studies have shown that brain-derived neurotrophic factor (BDNF) enhances bone/cementum-related protein gene expression through the TrkB-c-Raf-ERK1/2-Elk-1 signaling pathway in cementoblasts, which play a critical role establishment of functional periodontal ligament. To clarify how BDNF regulates survival we examined its effects on cell death induced by serum starvation immortalized human cementoblast-like (HCEM) cells. inhibited HCEM Small-interfering RNA (siRNA) for TRKB,...
Background: Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration with variable efficacy. EMD application results in significantly higher frequencies of sites without clinical signs inflammation; additionally, patients receiving therapy report less post‐treatment discomfort. However, there are few reports that focus on defining the biologic mechanisms for observed anti‐inflammatory effects EMD. The aim this study was evaluate influence...
Central mucoepidermoid carcinoma (MEC) poses a diagnostic challenge because of its rarity and histological overlap with glandular odontogenic cyst (GOC). In MEC both salivary glands jaws, MAML2 arrangement has been well known as the specific gene alteration. We report case central arising from GOC diagnosed by fusion gene. A 57‐year‐old male presented multilocular cystic lesion in left molar region mandible. Histopathologically, multiple cysts lined thin cuboidal or non‐keratinized squamous...
Background: Cementum formation is considered to be a critical event for successful regeneration of periodontal tissues. Cementoblasts share many characteristics with osteoblasts. Prostaglandin E 2 (PGE ) an important local factor in bone metabolism. Although the effects PGE on osteoblasts are well known, its cementoblasts have not yet been established. We examined proliferation and differentiation mouse cementoblast cell line, OCCM‐30 cells. Methods: cells were treated three concentrations...
Periodontitis causes periodontal tissue destruction and results in physiological tooth dysfunction. Therefore, regeneration is ideal therapy for periodontitis. Mesenchymal stem cells (MSCs) are useful regenerative as they can differentiate into cells; however, the underlying regulatory mechanism unclear. In this study, we attempted to identify genes involved cell differentiation clarify effective therapy.The cementum ligament play important roles function. critical therapy. transplantation...
Previously, we reported that brain-derived neurotrophic factor (BDNF) enhances periodontal tissue regeneration by inducing ligament cell proliferation in vivo. In addition, the down growth of gingival epithelial cells, which comprises a major obstacle to regeneration, was not observed. However, underlying molecular mechanism is still unclear. Therefore, this study aimed investigate effect BDNF on and apoptosis human (HPL) cells (OBA9 cells) explore vitro. HPL dominantly expressed receptor,...
The Wilms' tumor 1 gene (WT1) was originally isolated and described as the responsible for tumor. Although there is growing evidence linking overexpression of WT1 to tumorigenesis, no reports on ameloblastoma are available at present. aim this study examine expression in various histological subtypes tissue specimens human cell lines. Immunohistochemical analyses were performed a total 168 cases ameloblastoma, one case ameloblastic carcinoma, five tooth germs (control). Three immortalized...
A large number of individuals have halitosis. The total amount volatile sulfur compounds, which are the main cause halitosis, has been correlated with periodontitis following bacterial infection. In this study, Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients halitosis by amplification 16S rRNA, and ability Pg to produce methyl mercaptan (CH3 SH) determined clarify relationship between infection.CH3 SH concentrations were measured in using...
The aim of the study was to evaluate oral environment and taste function Japanese HIV-infected patients treated with antiretroviral therapy. Their median age 73 taking anti-HIV drugs 46 years. period 30 months. condition evaluated by measurement moisture, amount saliva secretion, number bacteria, presence candida, a test, missing teeth. levels moisture secreted were significantly lower in group than healthy volunteer (control) group. showed more robust decrease sensation control teeth higher...