A5103164412- Periodontal Regeneration and Treatments
- Oral microbiology and periodontitis research
- dental development and anomalies
- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Oral and gingival health research
- Inflammatory mediators and NSAID effects
- Oral Health Pathology and Treatment
- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- Dental Trauma and Treatments
- Dental Health and Care Utilization
- Salivary Gland Disorders and Functions
- Oral and Maxillofacial Pathology
- Dental Radiography and Imaging
- Streptococcal Infections and Treatments
- HIV/AIDS oral health manifestations
- Connective Tissue Growth Factor Research
- Electrospun Nanofibers in Biomedical Applications
- Peptidase Inhibition and Analysis
- Antimicrobial Resistance in Staphylococcus
- Laser Applications in Dentistry and Medicine
- COVID-19 Clinical Research Studies
Kagoshima University
2015-2024
Kagoshima University Hospital
2017
Hyogo Medical University
2013
Health Sciences University of Hokkaido
2012
Noguchi Hospital
2012
British Society of Periodontology
1995-2011
Tokyo Medical and Dental University
1995-2007
Hiroshima University
2007
National Center for Geriatrics and Gerontology
2007
University of Washington
2007
Stem cells have been used for regenerative therapies in various fields. The proportion of that possess stem cell properties human periodontal ligament (PDL) is not yet well understood. In this study, we quantitatively characterized PDL to clarify their properties, including self-renewal, multipotency, and marker expression.PDL were obtained from extracted premolar or wisdom teeth, following which a proliferation assay differentiation immunostaining STRO-1, fluorescence-activated sorter...
The aim of this study was to evaluate the effect surface roughness on initial attachment mouse osteoblast-like cells ceriastabilized zirconia/alumina nanocomposite (NANOZR) and yttria-stabilized zirconia (3Y-TZP) in comparison those pure titanium (Ti) alumina oxide (AO). Specimens with smooth rough surfaces were prepared by grinding diamond paper or sandblasting, respectively. For four substrates examined, number attached specimens significantly higher than that (p < 0.05). Integrin alpha(5)...
ABSTRACT We investigated the efficacy, safety, and clinical significance of trafermin, a recombinant human fibroblast growth factor (rhFGF)-2, for periodontal regeneration in intrabony defects Phase III trials. Study A, multicenter, randomized, double-blind, placebo-controlled study, was conducted at 24 centers. Patients with periodontitis 4-mm 3-mm or deeper probing pocket depth defects, respectively, were included. A total 328 patients randomly assigned (2:1) to receive 0.3% rhFGF-2...
In-vitro data have shown that cross-linked hyaluronic acid (HA) enhances the proliferative and migratory properties of cells involved in periodontal wound healing/regeneration, stabilizes blood clot, reduces inflammatory response, facilitates angiogenesis. The aim this study was to histologically evaluate effects HA alone or combined with a collagen matrix (CM) on healing/regeneration intrabony defects.Two-wall defects (5 mm wide, 5 deep) were surgically created at distal mesial aspects...
The purpose of this study was to evaluate the effects acid-etched titanium on biological responses osteoblast-like MC3T3-E1 cells. Four types treatments (polishing, sandblasting, concentrated H2SO4 etching, and etching with vacuum firing) were carried out surfaces commercially pure (cpTi) disks. cells then cultured treated cpTi surfaces. Through surface roughness measurement SEM analysis, it found that showed higher values than sandblasted ones. Scanning electron microscope analysis disks...
Hasegawa-Nakamura K, Tateishi F, Nakamura T, Nakajima Y, Kawamata Douchi Hatae M, Noguchi K. The possible mechanism of preterm birth associated with periodontopathic Porphyromonas gingivalis. J Periodont Res 2011; 46: 497–504. © 2011 John Wiley & Sons A/S Background and Objective: Previous studies have shown that gingivalis is found in the amniotic fluid placentae pregnant women some obstetric diseases. However, biological effects P. on intrauterine tissues remain unclear. aim this study was...
The aim of the present study was to evaluate effect a basic fibroblast growth factor (bFGF) candidate treatment on periodontal healing in two-wall intrabony defects dogs.Two-wall (5 x 5 mm) were created surgically distal and mesial sides bilateral mandibular second fourth premolars four Beagle dogs. bFGF, enamel matrix derivative (EMD) platelet-derived with beta-tricalcium phosphate (PDGF/beta-TCP) treatments, sham-surgery (OFD) rotated among each animal, EMD PDGF/beta-TCP serving as...
We investigated the prevalences and risk factors for peri-implant diseases in Japanese adult dental patients attending a follow-up visit at hospitals or clinics as part of their maintenance program. This cross-sectional multicenter study enrolled with implants who attended regular check-ups periodontal program during period from October 2012 through September 2013. Patients least 3 years loading time were included study. The condition tissue was examined classified into following categories:...
Abstract Aim To clinically and histologically evaluate in dogs the healing of gingival recessions treated with coronally advanced flap (CAF) or without cross‐linked hyaluronic acid (HA). Materials methods Gingival recession defects were surgically created on vestibular side both maxillary canines 8 dogs. After weeks plaque accumulation, 16 chronic randomly either CAF alone HA‐gel (CAF/HA). Clinical histological outcomes evaluated at 10 post‐surgically. Results Compared to baseline, clinical...
To histologically evaluate the effects of cross-linked hyaluronic acid (xHyA) with or without a collagen matrix (CM) on periodontal wound healing/regeneration in class III furcation defects dogs.Class were surgically created mandibular premolars six beagle dogs. The randomly treated as follows: open flap debridement (OFD) + CM (CM), OFD xHyA (xHyA), (xHyA/CM) and alone (OFD). At 10 weeks, animals euthanized for histological evaluation.The newly formed bone areas (4.04 ± 1.51 mm2 ) xHyA/CM...
Abstract In this study, near-infrared (NIR) spectroscopy, a measurement method not widely used in dentistry, was to monitor the synthesis of calcium phosphate cement. Tetra and di dihydrate were mixed at Ca/P ratio 1.67, water added dropwise induce phase transition HAp. The reaction conditions evaluated by measuring NIR spectra every minute for 60 min 20 24 h. obtained analyzed using singular value decomposition. synthesized apatite characterized XRD, FT-IR, SEM. self-setting cement system,...
Objective and background: It is not well known how periodontopathic bacteria colonize in the oral cavity during childhood. The purpose of this study was to investigate distribution cavities children their parents relationship between bacterial findings clinical parameters. Methods: Fifty‐six (mean age: 8.3 ± 3.5, range: 1–15 years), including 15 with deciduous dentition, 26 mixed dentition permanent participated study. Whole saliva dental plaque whole were collected for detection seven...
It has been reported that both prostaglandin E2 (PGE2) and Er:YAG laser irradiation accelerate wound healing. The stimulatory action of seems to occur during the proliferative stage healing by stimulation cyclooxygenase-2 (COX-2), which are crucial early mediators in natural process. We have then investigated effect on PGE2 production COX-2 gene expression human gingival fibroblast vitro.Cultured fibroblasts were exposed low-power with an energy density 3.37 J/cm2. amount was measured...
Tannerella forsythia is an anaerobic, Gram-negative bacterium involved in the so-called "red complex," which associated with severe and chronic periodontitis. The surface layer (S-layer) of T. composed cell glycoproteins, such as TfsA TfsB, known to play a role adhesion/invasion suppression proinflammatory cytokine expression. Here we investigated association this S-layer serum resistance coaggregation other oral bacteria. growth S-layer-deficient mutant bacterial medium containing more than...
Periodontal disease is assessed and its progression determined via observations on a site-by-site basis. data are complex structured in multiple levels; thus, applying summary statistical approach (i.e., the mean) for site-level evaluations results loss of information. Previous studies have shown availability mixed effects modeling. However, clinically beneficial information periodontal during follow-up period not available. We conducted multicenter prospective cohort study. Using modeling,...
Background and Objective A diagnosis of periodontitis progression is presently limited to clinical parameters such as attachment loss radiographic imaging. The aim this multicenter study was monitor disease in patients with chronic during a 24‐mo follow‐up program evaluate the amount bacteria saliva corresponding IgG titers serum for determining diagnostic usefulness each indicating stability. Material Methods total 163 who received trimonthly care were observed 24 mo. salivary content...
Bone morphogenetic protein (BMP)9 has been reported to be the most potent BMP induce bone formation. However, details of BMP9‐transduced intracellular signaling remain ambiguous. Here, we have investigated signal transduction mechanisms BMP9 in comparison BMP2, another inducer formation, osteoblasts. In a mouse osteoblast cell line, induced higher mRNA levels alkaline phosphatase (ALP) and runt‐related transcription factor 2 (Runx2) than BMP2 within h. Unlike rapid phosphorylation glycogen...
Abstract Objectives To evaluate periodontal wound healing following scaling and root planing (SRP) in conjunction with the application of sodium hypochlorite/amino acids cross-linked hyaluronic acid (xHyA) gels dogs. Materials Methods In four beagle dogs, 2-wall intrabony defects were created metal strips placed around teeth. Clinical parameters measured 4 weeks after plaque accumulation. The experimental surfaces subjected to SRP either subgingival a gel xHyA (test group) or alone (control...
Abstract Periodontal ligament (PDL) is a thin fibrous connective tissue located between two mineralized tissues, alveolar bone and cementum, which maintains constant width physiologically. The mechanisms by PDL resists mineralization are not well understood. Twist basic helix loop protein that plays central role in regulation of early osteogenesis. We investigated the localization compared expression osteoblast‐related genes cells with those osteoblast‐like presence or absence recombinant...
Destruction of cementum and alveolar bone is the main causative event for exfoliation teeth as a consequence periodontitis. Prostaglandin E 2 (PGE ) PGE receptor subtypes (EPs) play an important role in modulating osteoblast-mediated osteoclastogenesis; however, no information available on EPs regulating cementoblast-mediated cementoclastogenesis. We hypothesized that -EPs pathway also regulates cementoblasts’ ability to activate cementoclasts. For these studies, OCCM-30 cells (a mouse...