A5080871072- Underwater Acoustics Research
- Underwater Vehicles and Communication Systems
- Periodontal Regeneration and Treatments
- Oral microbiology and periodontitis research
- Seismic Waves and Analysis
- Speech and Audio Processing
- Acoustic Wave Phenomena Research
- Marine animal studies overview
- Oceanographic and Atmospheric Processes
- Laser-Plasma Interactions and Diagnostics
- Geophysical Methods and Applications
- Seismic Imaging and Inversion Techniques
- Mesenchymal stem cell research
- Flow Measurement and Analysis
- Laser-induced spectroscopy and plasma
- Dental Radiography and Imaging
- Bone Tissue Engineering Materials
- TGF-β signaling in diseases
- Pharmacy and Medical Practices
- Bone and Dental Protein Studies
- Ultrasonics and Acoustic Wave Propagation
- Oral and gingival health research
- Laser Design and Applications
- Engineering Applied Research
- Laser-Matter Interactions and Applications
Kagoshima University
2013-2024
British Society of Periodontology
2011-2023
Osaka University of Pharmaceutical Sciences
2017-2021
Kagoshima University Hospital
2017-2018
University of Fukui Hospital
1996-2014
Panasonic (Japan)
2002-2014
National Defense Academy of Japan
2004-2013
National Defence Academy
2004-2012
Osaka University
1986-2012
Okayama University
2012
Orexin-A and -B are recently identified potent orexigenic peptides that derived from the same precursor peptide highly specifically localized in neurons located lateral hypothalamic area, a region classically implicated feeding behavior. We cloned whole length of human prepro-orexin gene corresponding cDNA. The mRNA was predicted to encode 131-residue (prepro-orexin). consists two exons one intron distributed over 1432 base pairs. 143-base pair first exon includes 5′-untranslated small part...
Abstract Bone grafts are typically categorized into four categories: autografts, allografts, xenografts, and synthetic alloplasts. While it was originally thought that all bone should be slowly resorbed replaced with native over time, accumulating evidence has in fact suggested the use of nonresorbable xenografts is favored for certain clinical indications. Thus, many clinicians take advantage properties/features various indications, such as contour augmentation, sinus grafting, guided...
In-vitro data have shown that cross-linked hyaluronic acid (HA) enhances the proliferative and migratory properties of cells involved in periodontal wound healing/regeneration, stabilizes blood clot, reduces inflammatory response, facilitates angiogenesis. The aim this study was to histologically evaluate effects HA alone or combined with a collagen matrix (CM) on healing/regeneration intrabony defects.Two-wall defects (5 mm wide, 5 deep) were surgically created at distal mesial aspects...
Background and Objective: Although some functions characterizations of human periodontal ligament (hPDL) cells have been reported, the role hPDL in disease is poorly understood. We previously reported that produce many kinds inflammatory cytokines by stimulation with Prevotella intermedia . In this study, we examined production stimulated Porphyromonas gingivalis as compared P. Material Methods: cultured Dulbecco's modified Eagles's medium (DMEM) containing 10% fetal bovine serum (FBS)...
Growth/differentiation factor-5 (GDF-5), a member of the transforming growth factor-beta superfamily, shows close structural relationship to bone morphogenetic proteins and plays crucial roles in skeletal, tendon, ligament morphogenesis. The mRNA encoding GDF-5 is also expressed during odontogenesis, especially dental follicle tissue. While this suggests that participates formation alveolar periodontal ligament, cementum, root, physiologic role these tissues adulthood remains unclear. We...
Hasegawa-Nakamura K, Tateishi F, Nakamura T, Nakajima Y, Kawamata Douchi Hatae M, Noguchi K. The possible mechanism of preterm birth associated with periodontopathic Porphyromonas gingivalis. J Periodont Res 2011; 46: 497–504. © 2011 John Wiley & Sons A/S Background and Objective: Previous studies have shown that gingivalis is found in the amniotic fluid placentae pregnant women some obstetric diseases. However, biological effects P. on intrauterine tissues remain unclear. aim this study was...
Abstract Aim To clinically and histologically evaluate in dogs the healing of gingival recessions treated with coronally advanced flap (CAF) or without cross‐linked hyaluronic acid (HA). Materials methods Gingival recession defects were surgically created on vestibular side both maxillary canines 8 dogs. After weeks plaque accumulation, 16 chronic randomly either CAF alone HA‐gel (CAF/HA). Clinical histological outcomes evaluated at 10 post‐surgically. Results Compared to baseline, clinical...
To histologically evaluate the effects of cross-linked hyaluronic acid (xHyA) with or without a collagen matrix (CM) on periodontal wound healing/regeneration in class III furcation defects dogs.Class were surgically created mandibular premolars six beagle dogs. The randomly treated as follows: open flap debridement (OFD) + CM (CM), OFD xHyA (xHyA), (xHyA/CM) and alone (OFD). At 10 weeks, animals euthanized for histological evaluation.The newly formed bone areas (4.04 ± 1.51 mm2 ) xHyA/CM...
Periodontal disease is assessed and its progression determined via observations on a site-by-site basis. data are complex structured in multiple levels; thus, applying summary statistical approach (i.e., the mean) for site-level evaluations results loss of information. Previous studies have shown availability mixed effects modeling. However, clinically beneficial information periodontal during follow-up period not available. We conducted multicenter prospective cohort study. Using modeling,...
Background and Objective A diagnosis of periodontitis progression is presently limited to clinical parameters such as attachment loss radiographic imaging. The aim this multicenter study was monitor disease in patients with chronic during a 24‐mo follow‐up program evaluate the amount bacteria saliva corresponding IgG titers serum for determining diagnostic usefulness each indicating stability. Material Methods total 163 who received trimonthly care were observed 24 mo. salivary content...
Bone morphogenetic protein (BMP)9 has been reported to be the most potent BMP induce bone formation. However, details of BMP9‐transduced intracellular signaling remain ambiguous. Here, we have investigated signal transduction mechanisms BMP9 in comparison BMP2, another inducer formation, osteoblasts. In a mouse osteoblast cell line, induced higher mRNA levels alkaline phosphatase (ALP) and runt‐related transcription factor 2 (Runx2) than BMP2 within h. Unlike rapid phosphorylation glycogen...
Abstract Objectives To evaluate periodontal wound healing following scaling and root planing (SRP) in conjunction with the application of sodium hypochlorite/amino acids cross-linked hyaluronic acid (xHyA) gels dogs. Materials Methods In four beagle dogs, 2-wall intrabony defects were created metal strips placed around teeth. Clinical parameters measured 4 weeks after plaque accumulation. The experimental surfaces subjected to SRP either subgingival a gel xHyA (test group) or alone (control...
Abstract Periodontal ligament fibroblasts (PDLFs) have osteogenic capacity, producing bone matrix proteins. Application of morphogenic proteins (BMPs) to PDLFs is a promising approach for periodontal regeneration. However, in chronic metabolic disorders, such as periodontitis, proper control accompanying inflammation essential optimizing the effects BMPs on PDLFs. We previously shown that low‐intensity pulsed ultrasound (LIPUS), medical technology induces mechanical stress using sound waves,...
Aim St John's Wort (SJW) enhances CYP3A4 activity and decreases blood concentrations of substrates. In this study, the effects SJW on a benzodiazepine hypnotic, quazepam, which is metabolized by CYP3A4, were examined. Methods Thirteen healthy subjects took single dose quazepam 15 mg after treatment with (900 day −1 ) or placebo for 14 days. The study was performed in randomized, placebo‐controlled, cross‐over design an interval 4 weeks between two treatments. Blood samples obtained during 48...
Abstract In the induction of contact hypersensitivity (CH) to an epicutaneously applied hapten, we have previously proposed that low doses hapten sensitize primarily through epidermal Langerhans' cells (LC), whereas high rely largely on dermal antigen‐presenting (APC). To examine this issue further, either or dinitrofluorobenzene (DNFB) mice. We observed reduced LC density at site after 12 h (nadir), which returned normal levels 24 only a dose hapten. When unrelated oxazolone, was painted...
Abstract Aim This study was undertaken to investigate the existence of a periodontopathic bacterium, Fusobacterium nucleatum , in chorionic tissues pregnant women, and effects F. on human chorion‐derived cells. Materials Methods Oral tissue samples were collected from 24 high‐risk women 15 normal women. The presence detected using polymerase chain reaction. Chorion‐derived cells Toll‐like receptor ( TLR )‐2 or ‐4 gene‐silenced stimulated with lipopolysaccharide LPS ). Interleukin IL )‐6...
To accomplish effective periodontal regeneration for defects, several regenerative methods using growth and differentiation factors, including bone morphogenetic proteins ( BMP s), have been developed. Bone protein‐9 exhibits the most potent osteogenic activity of this factor family. However, it is unclear whether exogenous ‐9 can induce in human ligament PDL ) fibroblasts. Here, we examined effects recombinant (rh) on osteoblastic fibroblasts vitro, compared with rh ‐2. Recombinant potently...
To evaluate periodontal wound healing/regeneration of one-wall intra-bony defects treated with recombinant human fibroblast growth factor-2 (rhFGF-2) and beta-tricalcium phosphate (β-TCP), carbonate apatite (CO3 Ap), or deproteinized bovine bone mineral (DBBM) in dogs.The stability rhFGF-2 adsorbed onto the substitutes was evaluated by Enzyme-Linked Immunosorbent Assay (ELISA). One-wall (5 × 5 mm) created five adult male beagle dogs were alone (rhFGF-2), β-TCP (rhFGF-2/β-TCP), CO3 Ap...