A5103212354- Oral microbiology and periodontitis research
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Response and Inflammation
- Oral Health Pathology and Treatment
- Diabetes and associated disorders
- Salivary Gland Disorders and Functions
- Periodontal Regeneration and Treatments
- Oral and gingival health research
- Adipose Tissue and Metabolism
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Proteoglycans and glycosaminoglycans research
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Fibroblast Growth Factor Research
- Extracellular vesicles in disease
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Oral health in cancer treatment
- T-cell and B-cell Immunology
- S100 Proteins and Annexins
- dental development and anomalies
- HIV/AIDS oral health manifestations
Kyushu University
2016-2025
Kyushu Dental University
2002-2024
Saga University
2024
Jikei University School of Medicine
2022
Hiroshima University
2008-2018
Okayama University Hospital
2017
Psychiatric Rehabilitation Association
2014
The Institute of Medical Science, Asahi Life Foundation
2011-2013
The University of Tokyo
2011-2013
RIKEN BioResource Research Center
2013
Background: Obesity is an important risk factor for diabetes, cardiovascular disease, and periodontal disease. Adipocytes appear to secrete proinflammatory cytokines which may be the molecules linking pathogenesis of these diseases. We evaluated relationship between obesity, diabetes mellitus insulin resistance as well plasma levels tumor necrosis alpha (TNFα) its soluble receptors (sTNFα) assess inflammation infections. Methods: The was examined in Third National Health Nutrition...
Accurate quantification of bacterial species in dental plaque is needed for microbiological diagnosis periodontal diseases. The present study was designed to assess the sensitivity, specificity and quantitativity real-time PCR using GeneAmp Sequence Detection System with two fluorescence chemistries. TaqMan probe reporter quencher dye, SYBR Green dye were used sources fluorescence. Primers probes Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia total...
Mesenchymal stem cell (MSC)–derived exosome plays a central role in the cell-free therapeutics involving MSCs and contents can be customized under disease-associated microenvironments. However, optimal MSC-preconditioning to enhance its therapeutic potential is largely unknown. Here, we show that preconditioning of gingival tissue-derived (GMSCs) with tumor necrosis factor-alpha (TNF-α) ideal for treatment periodontitis. TNF-α stimulation not only increased amount secreted from GMSCs, but...
Background: Tumor necrosis factor‐α (TNF‐α) may play an important role in insulin resistance. In this study, we hypothesized that TNF‐α produced due to periodontal inflammation synergistically affects resistance as well from adipose tissues insulin‐resistant type 2 diabetes patients. Therefore, understand the effects of antimicrobial therapy on serum concentration and subsequent metabolic control diabetes, examined diabetic status 13 type‐2 Methods: These patients were treated with local...
Summary Background The prevalence of atopic diseases has been increasing in developed countries. This could be explained by the hygiene hypothesis, which states that exposure to specific infections or endotoxins during infancy drives maturing immune system towards a Th1 phenotype and away from Th2 phenotype, is associated with allergic diseases. Toll‐like receptors (TLRs) play important roles signalling many pathogen‐related molecules endogenous proteins activation. Objective aim present...
Immunoregulatory properties of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are promising. Gingival tissue-derived MSCs (GMSCs) have unique immunoregulatory capacity and secrete large amounts EVs. Recent findings suggest that priming with inflammatory stimuli is an effective strategy for cell-free therapy. However, the precise mechanism by which contents EVs customized has not been fully elucidated. Here, we show derived from GMSCs primed a combination two...
Insulin resistance and hyperglycemia are risk factors for periodontitis poor wound healing in diabetes, which have been associated with selective loss of insulin activation the PI3K/Akt pathway gingiva. This study showed that mouse gingiva due to deletion smooth muscle fibroblast receptor (SMIRKO mice) or systemic metabolic changes induced by a high-fat diet (HFD) HFD-fed mice exacerbated periodontitis-induced alveolar bone loss, preceded delayed neutrophil monocyte recruitment impaired...
Elevated levels of C-reactive protein (CRP) and decreased plasma adiponectin are associated with increased risk atherosclerosis. Furthermore, recent observations suggested that tumor necrosis factor-alpha (TNF-alpha) suppressed each other's production. Since periodontal disease has been to act as a factor for atherosclerosis, we examined the effects antimicrobial treatment on CRP, adiponectin, TNF-alpha levels.Fifteen chronic periodontitis patients various systemic conditions at high...
Abstract Objectives: Tumour necrosis factor‐ α (TNF‐ ) and interleukin‐1 β (IL‐1 participate in the establishment of inflammatory lesions periodontitis. High production these cytokines may relate to severity There have already been several studies examining association between periodontitis single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs TNF‐α , −1031, −863 −857, variants which are observed a relatively large proportion Japanese, identified. The...
Gut microbiota alterations are associated with various disorders. In this study, gut changes were investigated in a methionine-choline-deficient (MCD) diet-induced nonalcoholic steatohepatitis (NASH) rodent model, and the effects of administering Lactobacillus casei strain Shirota (LcS) on development NASH also investigated. Mice divided into three groups, given normal chow diet (NCD), MCD diet, or plus daily oral administration LcS for 6 wk. analyses groups revealed that lactic acid...
The prevalence and severity of periodontitis are increased advanced in diabetes. Severe elicits adverse effects on diabetes by impairing insulin actions due to systemic microinflammation. Recent studies unveil the emerging findings molecular basis bidirectional relationship between In addition conventional mechanisms such as hyperglycemia, hyperlipidemia, chronic inflammation, deficient action may play a pathogenic role progression under Epidemiologically, from viewpoint effect diabetes,...
Selective recruitment of periodontal ligament cells to a previously exposed root surface is believed enhance regeneration. It has been hypothesized that competition from gingival fibroblasts may reduce the potential We compared migratory responses PDL and variety biologicals. Parallel experiments designed examine directed migration both (PDL cells) (GF) isolated same donors were conducted using Platelet Derived Growth Factor (PDGF), Insulin Like Factor-I, -II (IGF-I, -II), Epidermal (EGF),...
Chronic low-grade infection has been suggested to be associated with metabolic disorder such as diabetes. However, the molecular mechanism underlying this important association is largely unknown. The only clue established so far that many subjects exhibit elevated levels of C-reactive protein measured by highly sensitive assay. Here, we hypothesized adipocyte–macrophage interaction plays a key role in amplifying low grade level influencing disorders. presence macrophages abdominal adipose...
Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (Pin1) is a unique enzyme that associates with the pSer/Thr-Pro motif and catalyzes cis-trans isomerization. We identified Pin1 in immunoprecipitates of overexpressed IRS-1 myc FLAG tags mouse livers confirmed association between by not only overexpression experiments but also endogenously liver. The analysis using deletion- point-mutated constructs revealed WW domain located N terminus Ser-434 SAIN (Shc NPXY binding) to be involved...
Dipeptidyl peptidase IV (DPP-IV) expression in visceral adipose tissue is reportedly increased obese patients, suggesting an association of DPP-IV with inflammation. In this study, first, lipopolysaccharide (LPS)- or palmitate-induced elevations inflammatory cytokine mRNA expressions RAW264.7 macrophages were shown to be significantly suppressed by coincubation a inhibitor, anagliptin (10 μM), despite low the cells. Regarding molecular mechanism, LPS-induced degradation IκBα and...
Recently, clinical studies have shown the protective effects of sodium glucose co-transporter2 (SGLT2) inhibitors against progression diabetic nephropathy, but underlying molecular mechanisms remain unclear. Diabetic mice were prepared by injecting nicotinamide and streptozotocin, followed high-sucrose diet feeding (NA/STZ/Suc mice). The SGLT2 inhibitor canagliflozin was administered as a 0.03% (w/w) mixture in for 4 weeks. Then, various parameters on nephropathy investigated. Canagliflozin...
Epidemiological studies suggest that the severity of periodontitis is higher in people with diabetes than healthy individuals. Insulin resistance might play a crucial role pathogenesis multiple diabetic complications and reportedly induced gingiva rodents type 2 diabetes; however, molecular mechanisms underlying diabetes-related remain unclear. Therefore, we aimed to investigate whether endothelial insulin may contribute as well elucidate its mechanisms. We demonstrated treatment...
A major focus of studies that center on regeneration the periodontium is to determine efficacy use polypeptide growth factors. Platelet-derived factor has been reported be a possible agent for clinical use. PDGF various isoforms. Therefore, we decided study mitogenic and chemotactic responses human periodontal ligament (PDL) cells recombinant PDGF-AB, AA, BB. Addition each isoform in vitro mitogenesis assays induced PDL cell proliferation dose-dependent manner. The maximum effect was evident...