A5056676785- Oral microbiology and periodontitis research
- Immune Response and Inflammation
- Proteoglycans and glycosaminoglycans research
- Advanced MRI Techniques and Applications
- Bacterial Infections and Vaccines
- Cardiac Imaging and Diagnostics
- Salivary Gland Disorders and Functions
- Oral and gingival health research
- Pneumonia and Respiratory Infections
- Cardiovascular Function and Risk Factors
- Glycosylation and Glycoproteins Research
- Pleural and Pulmonary Diseases
- Oral Health Pathology and Treatment
- Cancer Research and Treatments
- Structural Engineering and Vibration Analysis
- Renal Diseases and Glomerulopathies
- Antibiotics Pharmacokinetics and Efficacy
- Streptococcal Infections and Treatments
- Medical Imaging and Pathology Studies
- Building materials and conservation
- Peptidase Inhibition and Analysis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Geotechnical Engineering and Underground Structures
- Neonatal Respiratory Health Research
Fukui-ken Saiseikai Hospital
2018-2024
Tokyo Metropolitan Komagome Hospital
2020-2024
Saiseikai Fukuoka General Hospital
2022
Gunma University Hospital
2013-2019
Gunma University
1990-2013
Nihon University
2013
Okayama University Hospital
2012
Hiroshima University
2010
Okayama University
1998-2008
Independent Administrative Institution National Research Institute for Cultural Properties
1990-2008
Accurate quantification of bacterial species in dental plaque is needed for microbiological diagnosis periodontal diseases. The present study was designed to assess the sensitivity, specificity and quantitativity real-time PCR using GeneAmp Sequence Detection System with two fluorescence chemistries. TaqMan probe reporter quencher dye, SYBR Green dye were used sources fluorescence. Primers probes Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia total...
We studied various biological activities of crystalline pertussigen and found that in mice as little 0.5 ng induced hypersensitivity to histamine, 8 40 leukocytosis, 2 increased production insulin, 0.1 immunoglobulin E G1 antibodies hen egg albumin, 9.5 susceptibility anaphylactic shock, the vascular permeability striated muscle. also Lewis rats 20 promoted induction hyperacute experimental allergic encephalomyelitis. Pertussigen given intraperitoneally was toxic at a dose 546 ng. Treatment...
We compared the protective activities of fimbrial hemagglutinin (FHA) and pertussigen their respective antibodies in mice infected intracerebrally with Bordetella pertussis. found that were protected by a 1.7-microgram/mouse dose which was free detectable FHA detoxified treatment glutaraldehyde. A preparation made from cells grown shake cultures did not contain demonstrable at 1.4 microgram/mouse. 10 microgram/mouse intracerebral infection, but it also sensitized to histamine 2...
Leukocytosis-promoting factor (LPF) was purified from the supernatant of a 5-day liquid culture Bordetella pertussis strains Tohama and no. 134. The preparations appeared to be filamentous molecules (2 by 40 nm) contained histamine-sensitizing hemagglutinin activities in addition leukocytosis-promoting activity. Active protection tests mice demonstrated strong protective 80 international units per mg protein LPF each strain toxoided mild Formalin treatment. Passive with anti-LPF sera...
Abstract Objectives Coronary microvascular functional and structural obstructions (CMVO) after percutaneous coronary intervention (PCI) are a major cause of poor clinical outcomes in patients with acute syndrome. This study aimed to noninvasively diagnose the presence CMVO using non-contrast multiparametric cardiac magnetic resonance (CMR) myocardial infarction (AMI) who underwent PCI. Methods We retrospectively enrolled consecutive AMI PCI subsequent acute-phase CMR at our hospital. The...
The leucocytosis-promoting factor was purified from the supernatant fluid of spent cultures Bordetella pertussis on solid medium. After precipitation at 67% saturation ammonium sulfate, extracted with a 1.0 m NaCl solution. Purification accomplished by starch block electrophoresis and sucrose density gradient centrifugation. preparation contained high activity, as small an amount 0.04 μg protein induced leucocytosis in mice. About 520-fold purification attained, re-recovery about 25%...
Denaturing gradient gel electrophoresis (DGGE) was applied to the microbiologic examination of subgingival plaque.The PCR primers were designed from conserved nucleotide sequences on 16S ribosomal RNA gene (16SrDNA) with GC rich clamp at 5'-end. Polymerase chain reaction (PCR) performed using and genomic DNAs typical periodontal bacteria. The generated 16SrDNA fragments separated by denaturing gel.Although sizes amplified DNA almost same among species, 16SrDNAs bacteria distinguished...
Annual change in the density of sulfur-oxidizing microorganisms on sandstone was enumerated to know effects deterioration stone materials Angkor monuments Cambodia. Samples were obtained from total 12 stations at Wat, Bayon, and Phnom Krom temples between 1998 2007. Sulfur-oxidizing a mineral salts medium supplemented with elemental sulfur as sole energy source had 101-105 MPN (g sample)-1. The samples collected Wat have tended decrease since 2002; other hand, relatively constant values been...
Interleukin (IL)-6 has an important role in inflammatory diseases. Lysosomal enzymes cathepsins are widely expressed as cysteine proteases regulating process. Caveolin-1 (Cav-1) is a scaffolding/regulatory membrane protein that interacts with signaling molecules. In this study, we investigated the of Cav-1 on (1) productivity, and (2) enzymatic activity cathepsin B L human gingival fibroblasts (HGFs) treated IL-6 presence soluble form receptor (sIL-6R). At first, established siRNA-mediated...
A method is described for crystallizing pertussigen from Bordetella pertussis. The crystalline material induced histamine hypersensitivity in mice at a dose of 0.5 ng protein and leukocytosis 100 was toxic 429 microgram. histamine-sensitizing activity the toxicity were as high ever reported.
Background: The pathogenesis of early‐onset periodontitis (EOP) can be explained by various host risk factors. Previous studies have focused on a single (among many possible) immunological factor and the association among factors has not been assessed. We comprehensively investigated associations multiple in EOP patients to further elucidate their role EOP. Methods: Sixty‐eight (50 generalized EOP, 18 localized EOP), 51 EOP‐suspected (S‐EOP), 43 adult (AP) patients, 36 periodontally healthy...
Cardiac cine magnetic resonance imaging (MRI) is a widely used technique for the noninvasive assessment of cardiac functions. Deep neural networks have achieved considerable progress in overcoming various challenges MRI analysis. However, deep learning models cannot be classification because limited data are available. To overcome this problem, features from image settings derived by handcrafting and addition other clinical to classical machine approach ensuring model fits device parameters...
The leukocytosis-promoting factor (LPF), purified from materials extracted agar in which Bordetella pertussis was grown, caused leukocytosis and an increased sensitivity to histamine when as little 0.04 μg of protein given intravenously mice. LPF adsorbed onto erythrocytes, causing hemagglutination. As 0.03 agglutinated chicken erythrocytes 0.05 ml a 0.5% suspension. physicochemical, biological, immunological properties indicated that leukocytosis, mice, agglutination are functions single substance.
Interleukin‐6 (IL‐6), frequently detected in periodontitis, is known to mediate important signals the inflammatory cylokine network. Gingival fibroblasts (GF) secrete cytokines upon stimulation with mediators. However, it not clear if GF respond IL‐6. We examined IL‐6 receptor gene expression GF. Furthermore, we tested whether are target cells for by examination of binding were found contain trace amounts mRNA (IL‐6R), but had high levels 130‐kDa glycoprotein (gp 130), which a signal...
Human Gingival Fibroblasts (HGF) may have an important role in the orchestration of immuno-participant cells infiltrating gingiva response to continuously recurring bacterial infection. To examine cytokine network regulating HGF-derived interleukin (IL)-8, a potent neutrophil chemotactic cytokine, we analyzed effects inflammatory cytokines alone and combination on IL-8 production by HGF. IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-6, were used as...
Two kinds of errors are frequently encountered during the construction cable-stayed bridges: one is force-application error in jacking cable forces, and other geometry controlling elevations girders. These normally kept within prescribed tolerances by adjusting forces. However, influence creep prestressed concrete bridges cannot be ignored. If this not considered, it possible to exceed allowable stay-force range at time when ends. This paper presents an optimization application which can...