- Building materials and conservation
- Microbial Community Ecology and Physiology
- Cassava research and cyanide
- Conservation Techniques and Studies
- Cultural Heritage Materials Analysis
- Polyamine Metabolism and Applications
- Neuroscience and Neuropharmacology Research
- Plant Pathogens and Fungal Diseases
- Pain Mechanisms and Treatments
- Wastewater Treatment and Nitrogen Removal
- Odor and Emission Control Technologies
- Pituitary Gland Disorders and Treatments
- Ophthalmology and Eye Disorders
- Palliative Care and End-of-Life Issues
- Lichen and fungal ecology
- Methane Hydrates and Related Phenomena
- Cerebrospinal fluid and hydrocephalus
- Enzyme Structure and Function
- Metal Extraction and Bioleaching
- Photoreceptor and optogenetics research
- Amino Acid Enzymes and Metabolism
- Stress Responses and Cortisol
- Genomics and Phylogenetic Studies
- Atmospheric and Environmental Gas Dynamics
- Anaerobic Digestion and Biogas Production
Independent Administrative Institution National Research Institute for Cultural Properties
2018-2025
Tokyo University of Agriculture and Technology
2016-2025
Tokyo Women's Medical University
1990-2021
Kagawa Prefectural College of Health Sciences
2020-2021
Kagawa University
2013
Ibaraki Children's Hospital
2010
National Institute of Advanced Industrial Science and Technology
1992-2007
Gifu Women's University
2006
Nagoya University
2005
Taisho Pharmaceutical (Japan)
1995
Abstract. For the past decade, observations of carbonyl sulfide (OCS or COS) have been investigated as a proxy for carbon uptake by plants. OCS is destroyed enzymes that interact with CO2 during photosynthesis, namely carbonic anhydrase (CA) and RuBisCO, where CA more important one. The majority sources to atmosphere are geographically separated from this large plant sink, whereas sinks co-located in ecosystems. drawdown can therefore be related without added complication emissions...
A facultatively chemolithotrophic thiocyanate-degrading bacterium, strain THI 011 T , which was previously isolated from activated sludge and tentatively named Thiobacillus sp., studied taxonomically phylogenetically. This bacterium utilizes thiocyanate as sole energy source the specific growth rate for chemolithoautotrophic with 0059 h -1 . Molecular phylogenetic relationships of to versutus members genus Paracoccus were elucidated by comparing 16S rRNA gene sequences. Binary sequence...
Comparison of both 16S rRNA coding sequences and DNA-DNA hybridization ten strains α-subclass Proteobacteria currently classified as Paracoccus denitrificans has shown that they fall into two groups which are distinct from each other at the species level. with published data on cytochrome c profiles in literature confirmed these observations enabled several also to be assigned groups. Group A comprises ATCC 1774T (the type strain P. denitrificans), LMD 22.2V, DSM 413T, 19367, 13543, 1404,...
A thiocyanate hydrolase that catalyzes the first step in degradation was purified to homogeneity from Thiobacillus thioparus, an obligate chemolithotrophic eubacterium metabolizing sulfate as energy source.The 52-fold by steps involving ammonium precipitation, DEAE-Sephacel column chromatography, and hydroxylapatite chromatography.The enzyme hydrolyzed 1 mol of form carbonyl sulfide ammonia follows: SCN-+ 2H20 COS + NH3 OH-.This is report describing hydrolysis enzyme.The had a molecular mass...
Carbonyl sulfide (COS) is an atmospheric trace gas leading to sulfate aerosol formation, thereby participating in the global radiation balance and ozone chemistry, but its biological sinks are not well understood. Thiobacillus thioparus strain THI115 can grow on thiocyanate (SCN(-)) as sole energy source. Previously, we showed that SCN(-) first converted COS by hydrolase T. THI115. In present work, purified, characterized, determined crystal structure of carbonyl (COSase), which responsible...
The temples of Angkor monuments including Thom and Bayon in Cambodia surrounding countries were exclusively constructed using sandstone. They are severely threatened by biodeterioration caused active growth different microorganisms on the sandstone surfaces, but knowledge microbial community composition biofilms is not available from this region. This study investigated diversity examining fresh old biodeteriorated bas-relief wall surfaces Temple analysis 16S 18S rRNA gene sequences. results...
The Bayon temple in Angkor Thom, Cambodia has shown serious deterioration and is subject to the formation of various pigmented biofilms. Because biofilms are damaging bas-reliefs, low reliefs engraved on surface sandstone, information about microbial community within them indispensable control biofilm colonization. PCR-denaturing gradient gel electrophoresis (DGGE) analysis samples from sandstone surfaces showed that bacterial members differed clearly those air had sequence similarity...
Abstract. For the past decade, observations of carbonyl sulfide (OCS or COS) have been investigated as a proxy for carbon uptake by plants. OCS is destroyed enzymes that interact with CO2 during photosynthesis, namely carbonic anhydrase (CA) and RuBisCO, where CA more important. The majority sources to atmosphere are geographically separated from this large plant sink, whereas sinks co-located in ecosystems. drawdown can therefore be related without added complication emissions comparable...
Carbonyl sulfide hydrolase (COSase) is a unique enzyme that exhibits high activity towards carbonyl (COS) but low carbonic anhydrase (CA) activity, despite belonging to the CA family. COSase was initially identified in sulfur‐oxidizing bacterium and later discovered ascomycete Trichoderma harzianum strain THIF08. The from T. has been recognized as key assimilation of gaseous COS, homologous genes are widely present not only Ascomycota also Basidiomycota. Here, we characterized COSases...
To characterize sulfate-producing microorganisms from the deteriorated sandstones of Angkor monuments in Cambodia, strains Mycobacterium spp. were isolated most probable number-positive cultures. All five able to use both elemental sulfur (S(0)) for chemolithoautotrophic growth and organic substances chemoorganoheterotrophic growth. Results phylogenetic phenotypic analyses indicated that all isolates rapid growers genus similar cosmeticum pallens. Chemolithoautotrophic was further examined...
Functional synapse elimination and strengthening are crucial developmental processes in the formation of precise neuronal circuits somatosensory system, but underlying alterations topographical organization not yet fully understood. To address this issue, we generated transgenic mice which afferent fibers originating from whisker-related brain region, called maxillary principal trigeminal nucleus (PrV2), were selectively visualized with genetically expressed fluorescent protein. We found...