Infertility caused by ovarian or tubal problems can be treated using In Vitro Fertilization and Embryo Transfer (IVF-ET); however, this is not possible for women with uterine loss malformations that require reconstruction the treatment of their infertility. study, we are first to report usefulness decellularized matrices as a scaffold reconstruction. Uterine tissues were extracted from Sprague Dawley (SD) rats either sodium dodecyl sulfate (SDS) high hydrostatic pressure (HHP) at optimized...

Mesenchymal stem cell (MSC) condensation contributes to membrane ossification by enhancing their osteodifferentiation. We investigated bone regeneration in rats using the human marrow-derived MSC-spheroids prepared rotation culture, without synthetic or exogenous biomaterials. Bilateral calvarial defects (8 mm) were created nude male rats; left-sided implanted with MSC-spheroids, β-tricalcium phosphate (β-TCP) granules, β-TCP granules + while right-sided served as internal controls....

Although a close connection between uterine regeneration and successful pregnancy in both humans mice has been consistently observed, its molecular basis remains unclear. We here established mouse model of decellularized matrix (DUM) transplantation. Resected uteri were processed with SDS to make DUMs without any intact cells. transplanted into the artificially induced defects, all layers recovered at DUM transplantation sites within month. In regenerated uteri, normal hormone responsiveness...

A series of 2,5-diaryl-3,4-diphenylsiloles, with various mono-substituted phenyl groups, extended pi-conjugated and heteroaryl groups as aryl at the 2,5-positions, has been prepared by a one-pot synthesis from bis(phenylethynyl)silanes based on intramolecular reductive cyclization followed palladium-catalyzed cross-coupling halides. Crystal structures chemical reactivities toward alkaline desilylation reactions have studied 2,5-bis(p-mono-substituted phenyl)silole derivatives to elucidate...

A medium perfusion system is expected to be beneficial for three-dimensional (3D) culture of engineered bone, not only by chemotransport enhancement but also mechanical stimulation. In this study, systems with either unidirectional or oscillatory flow were developed, and the effects different profiles on 3D culturing bone studied. Mouse osteoblast-like MC 3T3-E1 cells 3D-cultured porous ceramic scaffolds in vitro 6 days under static hydrodynamic conditions a flow. We found that, culture,...

Chondrocytes in articular cartilage synthesize collagen type II and large sulfated proteoglycans, whereas the same cells cultured monolayer (2D) dedifferentiate into fibroblastic express I small proteoglycans. On other hand, a pellet culture system was developed as method for preventing phenotypic modulation of chondrocytes promoting redifferentiation dedifferentiated ones. Because forms only one cell aggregate each tube by centrifugator, could not be applied to produce tissue-engineered...

Tissue engineering approaches have been clinically tried to repair damaged articular cartilages. It is an essential step uniformly seed chondrocytes into 3D scaffolds in order reconstruct tissue-engineered cartilages vitro, but the tissue could not provided with efficient cell seeding methods. Type I collagen used and known as a cytocompatible material, having recognition sites for integrins. Collagen gel encapsulating has making regenerated cartilages, it found difficult keep its original...

Hydrostatic pressure is one of the most frequently used mechanical stimuli in chondrocyte experiments. A variety hydrostatic loading devices have been cartilage cell However, no gas-controlled system with other than a low load was up to this time. Hence we polyolefin bag from which gas penetration confirmed. Chondrocytes were extracted bovine normal knee joint cartilage. After 3 passages, dedifferentiated chondrocytes applied form pellet. These pellets cultured chemically defined serum-free...

Due to the high water content of cartilage, hydrostatic pressure is likely one main physical stimuli sensed by chondrocytes. Whereas, in physiological range (0 around 10 MPa), exerts mostly pro-chondrogenic effects chondrocyte models, excessive pressures have been reported induce detrimental on such as increased apoptosis and inflammation, decreased cartilage marker expression. Though some genes modulated identified, global gene expression pattern still not investigated. In this study, using...

A polymer surface with excellent blood compatibility has been produced via spontaneous segregation of a block copolymer. The water-soluble (PME3MA) spontaneously segregates into hydrophobic and hydrophilic environments (see Figure), causing the blocks to expel biological substances including proteins, cells platelets flexible chain motion.

Abstract Perfusion culture systems have proven to be effective bioreactors for constructing tissue engineered bone in vitro , but existing circuit‐based perfusion are complicated and costly conditioned due the large medium volume required. A compact system artificial fabrication using oscillatory flow is described here. Mouse osteoblast‐like MC 3T3‐E1 cells were seeded at 1.5 × 10 6 cells/100 μL cultured days porous ceramic beta‐tricalcium phosphate scaffolds (10 mm diameter, 8 height) by...

Because patient bone defects are usually varied and complicated in geometry, it would be preferred to fabricate custom-made artificial grafts that anatomically specific individual defects. Using a rabbit femoral segment as reconstruction model, we successfully produced customized ceramic scaffolds by stereolithography, which not only had an correct external shape according computed tomography data but also contained interconnecting internal network of channels designed for perfusion culture....

Scaffolds play a key role in the process of regeneration and morphogenesis tissue or organ. We have developed novel sonication decellularization system to prepare decellularized bio-scaffolds short treatment time. The aim study is investigate condition that completely decellularize meniscus can be changed as well maintain biomechanical parameters scaffolds. samples were using treatment. treated evaluated histologically by EVG for cell removal, picrosirius red content collagen type I III,...

ABSTRACT Articular cartilage protects and lubricates joints for smooth motion transmission of loads. Owing to its high water content, chondrocytes within the are exposed levels hydrostatic pressure, which has been shown promote chondrocyte identity through unknown mechanisms. Here, we investigate effects pressure on state behavior, discover that application promotes quiescence prevents maturation towards hypertrophic state. Mechanistically, reduces amount trimethylated H3K9 (K3K9me3)-marked...

We investigated whether articular chondrocytes could form three-dimensional tissue-engineered cartilage in a rotational culture system without scaffold. A suspension of derived from Japanese white rabbits was inoculated into mold. Eight hours later, the cell mold showed aggregation, forming chondrocyte plate. The removed, and plate cultured under static conditions. After 7 days primary culture, dynamic conditions, using culture. 2-3 weeks maintained constant considered stable enough to be...

Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many methods, hydrostatic pressure (HP) method stands out due its low cytotoxicity superior ECM preservation compared traditional detergent methods. Conventionally, 980 MPa was utilized in HP decellularization, including first successful implementation...